PH sensitivity of carbon dioxide conversion catalyzed by isocitrate dehydrogenase

Shunxiang Xia, Ping Wang, Jungbae Kim

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Biological conversion of carbon dioxide has been examined with growing interest in recent years for its potential in reduction of greenhouse gas emission. While many conversion routes have been suggested and demonstrated, understanding the constraints and limits of such reaction routes, either in vivo or in vitro, is becoming increasingly important. The current work examines the pH sensitivity of the reaction catalyzed by isocitrate dehydrogenase with respect to its potentials in carbon capture and conversion via both experimental measurements and theoretical analysis. As a result, the reaction equilibrium constant determined experimentally shifted by a factor of ∼500-fold when pH changed between 5 and 9. Thermodynamic analysis agreed well with such a pH sensitivity, indicating the impact of pH on Gibbs free energy of the species involved in the reaction helped to shift the reaction equilibrium.

Original languageEnglish
Pages (from-to)102-107
Number of pages6
JournalGreenhouse Gases: Science and Technology
Volume5
Issue number1
DOIs
Publication statusPublished - 2015 Feb 1

Fingerprint

Isocitrate Dehydrogenase
Carbon capture
Equilibrium constants
Gibbs free energy
Gas emissions
Greenhouse gases
Carbon Dioxide
Carbon dioxide
carbon dioxide
Thermodynamics
greenhouse gas
thermodynamics
fold
carbon
Oxidoreductases
analysis

Keywords

  • biocatalysis
  • biosynthesis
  • carbon conversion
  • carbon dioxide
  • isocitrate dehydrogenase

ASJC Scopus subject areas

  • Environmental Chemistry
  • Environmental Engineering

Cite this

PH sensitivity of carbon dioxide conversion catalyzed by isocitrate dehydrogenase. / Xia, Shunxiang; Wang, Ping; Kim, Jungbae.

In: Greenhouse Gases: Science and Technology, Vol. 5, No. 1, 01.02.2015, p. 102-107.

Research output: Contribution to journalArticle

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