Pharmacological activity of chaga mushroom on extraction conditions and immunostimulating polysaccharide

Gil Hun Baek, Heon Sang Jeong, Hoon Kim, Taek Joon Yoon, Hyung Joo Suh, Kwang Won Yu

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To investigate the pharmacological activity of chaga mushroom (Inonotus obliquus) on extraction conditions, chaga was extracted using water (reflux at 50°C, decoction over 90°C, pressure at 121°C) or ethanol (reflux at 50, 70, or 90°C). When water extract was further fractionated into crude polysaccharide (IO-CP), yields of IO-CP (4.8~16.8%) were higher than those of ethanolic extracts (IO-E, 1.9~2.7%) at increased temperature. For antioxidant activity, crude polysaccharide (IO-CP-121) obtained by pressurized extraction showed the highest polyphenolic and flavonoid contents (35.10 mg TAE/g and 18.48 mg QE/g, respectively) as well as DPPH and ABTS free radical scavenging activities (26.08 and 27.99 mg AEAC/100 mg, respectively). Meanwhile, IO-CP-D (decoction) and IO-CP-50 (reflux) had more potent mitogenic effects (2.10- and 1.95-fold of saline control at 100 μg/mL) as well as intestinal immune system modulating activities (6.30- and 5.74-fold) compared to IO-CP-121, whereas ethanolic extracts showed no activity. Although no IO-CP showed cytotoxicity against RAW 264.7 cells at 0.1 mg/mL, IO-CP-121 significantly inhibited TNF-α and NO production as pro-inflammatory factors in LPS-stimulated RAW 264.7 cells (29.2 and 63.5%, respectively). Ethanolic extracts also showed no cytotoxicity at 0.1 mg/mL, whereas inhibition of TNF-α and NO production was significantly low compared to that of IO-CP-121. In addition, active IO-CP-D was further fractionated into an unadsorbed (IO-CP-I) and seven adsorbed fractions (IO-CP-II~VIII) by DEAE-Sepharose CL-6B column chromatography in order to isolate immunostimulating polysaccharide. IO-CP-II showed the most potent mitogenic effect and macrophage stimulating activity (4.51- and 1.64-fold, respectively). IO-CP-II mainly contained neutral sugars (61.86%) in addition to a small amount of uronic acid (2.96%), and component sugar analysis showed that IO-CP-II consisted mainly of Glc, Gal, and Man (molar ratio of 1.00:0.55:0.31). Therefore, extraction conditions affect the physiological activity of chaga, and immunostimulating polysaccharide fractionated from chaga by decoction is composed mainly of neutral sugars.

Original languageEnglish
Pages (from-to)1378-1387
Number of pages10
JournalJournal of the Korean Society of Food Science and Nutrition
Volume41
Issue number10
DOIs
Publication statusPublished - 2012

Fingerprint

Agaricales
mushrooms
Polysaccharides
polysaccharides
Pharmacology
extracts
sugars
cytotoxicity
Inonotus obliquus
Uronic Acids
uronic acids
Water

Keywords

  • Chaga mushroom
  • Crude polysaccharide
  • Extraction condition
  • Pharmacological activity
  • Solvent extract

ASJC Scopus subject areas

  • Nutrition and Dietetics
  • Food Science

Cite this

Pharmacological activity of chaga mushroom on extraction conditions and immunostimulating polysaccharide. / Baek, Gil Hun; Jeong, Heon Sang; Kim, Hoon; Yoon, Taek Joon; Suh, Hyung Joo; Yu, Kwang Won.

In: Journal of the Korean Society of Food Science and Nutrition, Vol. 41, No. 10, 2012, p. 1378-1387.

Research output: Contribution to journalArticle

Baek, Gil Hun ; Jeong, Heon Sang ; Kim, Hoon ; Yoon, Taek Joon ; Suh, Hyung Joo ; Yu, Kwang Won. / Pharmacological activity of chaga mushroom on extraction conditions and immunostimulating polysaccharide. In: Journal of the Korean Society of Food Science and Nutrition. 2012 ; Vol. 41, No. 10. pp. 1378-1387.
@article{5a2ec02dad834b2792f120e81cc5ab97,
title = "Pharmacological activity of chaga mushroom on extraction conditions and immunostimulating polysaccharide",
abstract = "To investigate the pharmacological activity of chaga mushroom (Inonotus obliquus) on extraction conditions, chaga was extracted using water (reflux at 50°C, decoction over 90°C, pressure at 121°C) or ethanol (reflux at 50, 70, or 90°C). When water extract was further fractionated into crude polysaccharide (IO-CP), yields of IO-CP (4.8~16.8{\%}) were higher than those of ethanolic extracts (IO-E, 1.9~2.7{\%}) at increased temperature. For antioxidant activity, crude polysaccharide (IO-CP-121) obtained by pressurized extraction showed the highest polyphenolic and flavonoid contents (35.10 mg TAE/g and 18.48 mg QE/g, respectively) as well as DPPH and ABTS free radical scavenging activities (26.08 and 27.99 mg AEAC/100 mg, respectively). Meanwhile, IO-CP-D (decoction) and IO-CP-50 (reflux) had more potent mitogenic effects (2.10- and 1.95-fold of saline control at 100 μg/mL) as well as intestinal immune system modulating activities (6.30- and 5.74-fold) compared to IO-CP-121, whereas ethanolic extracts showed no activity. Although no IO-CP showed cytotoxicity against RAW 264.7 cells at 0.1 mg/mL, IO-CP-121 significantly inhibited TNF-α and NO production as pro-inflammatory factors in LPS-stimulated RAW 264.7 cells (29.2 and 63.5{\%}, respectively). Ethanolic extracts also showed no cytotoxicity at 0.1 mg/mL, whereas inhibition of TNF-α and NO production was significantly low compared to that of IO-CP-121. In addition, active IO-CP-D was further fractionated into an unadsorbed (IO-CP-I) and seven adsorbed fractions (IO-CP-II~VIII) by DEAE-Sepharose CL-6B column chromatography in order to isolate immunostimulating polysaccharide. IO-CP-II showed the most potent mitogenic effect and macrophage stimulating activity (4.51- and 1.64-fold, respectively). IO-CP-II mainly contained neutral sugars (61.86{\%}) in addition to a small amount of uronic acid (2.96{\%}), and component sugar analysis showed that IO-CP-II consisted mainly of Glc, Gal, and Man (molar ratio of 1.00:0.55:0.31). Therefore, extraction conditions affect the physiological activity of chaga, and immunostimulating polysaccharide fractionated from chaga by decoction is composed mainly of neutral sugars.",
keywords = "Chaga mushroom, Crude polysaccharide, Extraction condition, Pharmacological activity, Solvent extract",
author = "Baek, {Gil Hun} and Jeong, {Heon Sang} and Hoon Kim and Yoon, {Taek Joon} and Suh, {Hyung Joo} and Yu, {Kwang Won}",
year = "2012",
doi = "10.3746/jkfn.2012.41.10.1378",
language = "English",
volume = "41",
pages = "1378--1387",
journal = "Journal of the Korean Society of Food Science and Nutrition",
issn = "1226-3311",
publisher = "Korean Society of Food Science and Nutrition",
number = "10",

}

TY - JOUR

T1 - Pharmacological activity of chaga mushroom on extraction conditions and immunostimulating polysaccharide

AU - Baek, Gil Hun

AU - Jeong, Heon Sang

AU - Kim, Hoon

AU - Yoon, Taek Joon

AU - Suh, Hyung Joo

AU - Yu, Kwang Won

PY - 2012

Y1 - 2012

N2 - To investigate the pharmacological activity of chaga mushroom (Inonotus obliquus) on extraction conditions, chaga was extracted using water (reflux at 50°C, decoction over 90°C, pressure at 121°C) or ethanol (reflux at 50, 70, or 90°C). When water extract was further fractionated into crude polysaccharide (IO-CP), yields of IO-CP (4.8~16.8%) were higher than those of ethanolic extracts (IO-E, 1.9~2.7%) at increased temperature. For antioxidant activity, crude polysaccharide (IO-CP-121) obtained by pressurized extraction showed the highest polyphenolic and flavonoid contents (35.10 mg TAE/g and 18.48 mg QE/g, respectively) as well as DPPH and ABTS free radical scavenging activities (26.08 and 27.99 mg AEAC/100 mg, respectively). Meanwhile, IO-CP-D (decoction) and IO-CP-50 (reflux) had more potent mitogenic effects (2.10- and 1.95-fold of saline control at 100 μg/mL) as well as intestinal immune system modulating activities (6.30- and 5.74-fold) compared to IO-CP-121, whereas ethanolic extracts showed no activity. Although no IO-CP showed cytotoxicity against RAW 264.7 cells at 0.1 mg/mL, IO-CP-121 significantly inhibited TNF-α and NO production as pro-inflammatory factors in LPS-stimulated RAW 264.7 cells (29.2 and 63.5%, respectively). Ethanolic extracts also showed no cytotoxicity at 0.1 mg/mL, whereas inhibition of TNF-α and NO production was significantly low compared to that of IO-CP-121. In addition, active IO-CP-D was further fractionated into an unadsorbed (IO-CP-I) and seven adsorbed fractions (IO-CP-II~VIII) by DEAE-Sepharose CL-6B column chromatography in order to isolate immunostimulating polysaccharide. IO-CP-II showed the most potent mitogenic effect and macrophage stimulating activity (4.51- and 1.64-fold, respectively). IO-CP-II mainly contained neutral sugars (61.86%) in addition to a small amount of uronic acid (2.96%), and component sugar analysis showed that IO-CP-II consisted mainly of Glc, Gal, and Man (molar ratio of 1.00:0.55:0.31). Therefore, extraction conditions affect the physiological activity of chaga, and immunostimulating polysaccharide fractionated from chaga by decoction is composed mainly of neutral sugars.

AB - To investigate the pharmacological activity of chaga mushroom (Inonotus obliquus) on extraction conditions, chaga was extracted using water (reflux at 50°C, decoction over 90°C, pressure at 121°C) or ethanol (reflux at 50, 70, or 90°C). When water extract was further fractionated into crude polysaccharide (IO-CP), yields of IO-CP (4.8~16.8%) were higher than those of ethanolic extracts (IO-E, 1.9~2.7%) at increased temperature. For antioxidant activity, crude polysaccharide (IO-CP-121) obtained by pressurized extraction showed the highest polyphenolic and flavonoid contents (35.10 mg TAE/g and 18.48 mg QE/g, respectively) as well as DPPH and ABTS free radical scavenging activities (26.08 and 27.99 mg AEAC/100 mg, respectively). Meanwhile, IO-CP-D (decoction) and IO-CP-50 (reflux) had more potent mitogenic effects (2.10- and 1.95-fold of saline control at 100 μg/mL) as well as intestinal immune system modulating activities (6.30- and 5.74-fold) compared to IO-CP-121, whereas ethanolic extracts showed no activity. Although no IO-CP showed cytotoxicity against RAW 264.7 cells at 0.1 mg/mL, IO-CP-121 significantly inhibited TNF-α and NO production as pro-inflammatory factors in LPS-stimulated RAW 264.7 cells (29.2 and 63.5%, respectively). Ethanolic extracts also showed no cytotoxicity at 0.1 mg/mL, whereas inhibition of TNF-α and NO production was significantly low compared to that of IO-CP-121. In addition, active IO-CP-D was further fractionated into an unadsorbed (IO-CP-I) and seven adsorbed fractions (IO-CP-II~VIII) by DEAE-Sepharose CL-6B column chromatography in order to isolate immunostimulating polysaccharide. IO-CP-II showed the most potent mitogenic effect and macrophage stimulating activity (4.51- and 1.64-fold, respectively). IO-CP-II mainly contained neutral sugars (61.86%) in addition to a small amount of uronic acid (2.96%), and component sugar analysis showed that IO-CP-II consisted mainly of Glc, Gal, and Man (molar ratio of 1.00:0.55:0.31). Therefore, extraction conditions affect the physiological activity of chaga, and immunostimulating polysaccharide fractionated from chaga by decoction is composed mainly of neutral sugars.

KW - Chaga mushroom

KW - Crude polysaccharide

KW - Extraction condition

KW - Pharmacological activity

KW - Solvent extract

UR - http://www.scopus.com/inward/record.url?scp=84922686757&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84922686757&partnerID=8YFLogxK

U2 - 10.3746/jkfn.2012.41.10.1378

DO - 10.3746/jkfn.2012.41.10.1378

M3 - Article

VL - 41

SP - 1378

EP - 1387

JO - Journal of the Korean Society of Food Science and Nutrition

JF - Journal of the Korean Society of Food Science and Nutrition

SN - 1226-3311

IS - 10

ER -