Pharmacology of intracisternal or intrathecal glycine, muscimol, and baclofen in strychnine-induced thermal hyperalgesia of mice

Il Ok Lee, Jin Kook Son, Eui Sung Lim, Yeon Soo Kim

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Glycine and γ-aminobutyric acid (GABA) are localized and released by the same interneurons in the spinal cord. Although the effects of glycine and GABA on analgesia are well known, little is known about the effect of GABA in strychnine-induced hyperalgesia. To investigate the effect of GABA and the role of the glycine receptor in thermal hyperalgesia, we designed an experiment involving the injection of muscimol (a GABA A receptor agonist), baclofen (a GABA B receptor agonist) or glycine with strychnine (strychnine sensitive glycine receptor antagonist). Glycine, muscimol, or baclofen with strychnine was injected into the cisterna magna or lumbar subarachnoidal spaces of mice. The effects of treatment on strychnine-induced heat hyperalgesia were observed using the pain threshold index via the hot plate test. The dosages of experimental drugs and strychnine we chose had no effects on motor behavior in conscious mice. Intracisternal or intrathecal administration of strychnine produced thermal hyperalgesia in mice. Glycine antagonize the effects of strychnine, whereas, muscimol or baclofen does not. Our results indicate that glycine has anti-thermal hyperalgesic properties in vivo; and GABA receptor agonists may lack the binding abilities of glycine receptor antagonists with their sites in the central nervous system.

Original languageEnglish
Pages (from-to)1371-1377
Number of pages7
JournalJournal of Korean Medical Science
Volume26
Issue number10
DOIs
Publication statusPublished - 2011 Oct 1

Fingerprint

Strychnine
Muscimol
Baclofen
Hyperalgesia
Glycine
Aminobutyrates
Pharmacology
Glycine Receptors
Glycine Agents
Hot Temperature
Cisterna Magna
GABA-B Receptors
Pain Threshold
Aptitude
Interneurons
Analgesia
Spinal Cord
Central Nervous System

Keywords

  • Gamma-aminobutyric acid
  • Hyperalgesia
  • Intracisternal
  • Intrathecal drug delivery
  • Strychnine

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Pharmacology of intracisternal or intrathecal glycine, muscimol, and baclofen in strychnine-induced thermal hyperalgesia of mice. / Lee, Il Ok; Son, Jin Kook; Lim, Eui Sung; Kim, Yeon Soo.

In: Journal of Korean Medical Science, Vol. 26, No. 10, 01.10.2011, p. 1371-1377.

Research output: Contribution to journalArticle

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