Phase contrast radiography of Lewy bodies in Parkinson disease

Seong Beom Koh, Sang-Il Suh, D. H. Lee, Aeree Kim, Chil Hwan Oh, Joon Shik Yoon, B. M. Weon, S. K. Seol, A. R. Pyun, J. H. Je, Y. Hwu, G. Margaritondo

Research output: Contribution to journalArticle

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Abstract

Parkinson's disease (PD), defined as a neurodegenerative disorder, is characterized by the loss of dopaminergic neurons and the presence of Lewy bodies in neurons. Morphological study of Lewy bodies is important to identify the causes and the processes of PD. Here, we investigate a possibility of phase contrast radiography using coherent synchrotron X-rays to explore the microscopic details of Lewy bodies in thick (∼3 mm) midbrain tissues. Autopsied midbrain tissues of a PD patient were sliced in 3 mm thickness and then examined using synchrotron X-rays from the 7B2 beamline of the Pohang Light Source. Refraction-enhanced phase contrast radiography and microtomography were adopted to identify dark core and dim edge of Lewy bodies in neurons. The morphology of Lewy bodies was clearly revealed by the phase contrast radiography in very thick (3 mm) midbrain tissues without any staining treatment. Three-dimensional volume rendered microtomography of the autopsied midbrain tissues demonstrates striking evidence that several Lewy bodies are agglomerated by dim edges in a neuron. We suggest that the phase contrast radiography could be a useful tool to morphologically investigate the causes or the processes in PD.

Original languageEnglish
Pages (from-to)566-569
Number of pages4
JournalNeuroImage
Volume32
Issue number2
DOIs
Publication statusPublished - 2006 Aug 15

Fingerprint

Lewy Bodies
Radiography
Parkinson Disease
Mesencephalon
Synchrotrons
Neurons
X-Rays
Dopaminergic Neurons
Neurodegenerative Diseases
Staining and Labeling
Light

ASJC Scopus subject areas

  • Cognitive Neuroscience
  • Neurology

Cite this

Phase contrast radiography of Lewy bodies in Parkinson disease. / Koh, Seong Beom; Suh, Sang-Il; Lee, D. H.; Kim, Aeree; Oh, Chil Hwan; Yoon, Joon Shik; Weon, B. M.; Seol, S. K.; Pyun, A. R.; Je, J. H.; Hwu, Y.; Margaritondo, G.

In: NeuroImage, Vol. 32, No. 2, 15.08.2006, p. 566-569.

Research output: Contribution to journalArticle

Koh, SB, Suh, S-I, Lee, DH, Kim, A, Oh, CH, Yoon, JS, Weon, BM, Seol, SK, Pyun, AR, Je, JH, Hwu, Y & Margaritondo, G 2006, 'Phase contrast radiography of Lewy bodies in Parkinson disease', NeuroImage, vol. 32, no. 2, pp. 566-569. https://doi.org/10.1016/j.neuroimage.2006.04.217
Koh, Seong Beom ; Suh, Sang-Il ; Lee, D. H. ; Kim, Aeree ; Oh, Chil Hwan ; Yoon, Joon Shik ; Weon, B. M. ; Seol, S. K. ; Pyun, A. R. ; Je, J. H. ; Hwu, Y. ; Margaritondo, G. / Phase contrast radiography of Lewy bodies in Parkinson disease. In: NeuroImage. 2006 ; Vol. 32, No. 2. pp. 566-569.
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