Phenanthroquinolizidine alkaloids from the roots of Boehmeria pannosa potently inhibit hypoxia-inducible factor-1 in AGS human gastric cancer cells

Xing Fu Cai, Xuejun Jin, Dongho Lee, Young Taek Yang, Kyeong Lee, Young Soo Hong, Jeong Hyung Lee, Jung Joon Lee

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

A bioassay-guided phytochemical investigation on the methanol extract of Boehmeria pannosa, using a HIF-1-mediated reporter gene assay, led to the isolation of two phenanthroquinolizidine alkaloids, (-)-cryptopleurine (1) and (-)-(15R)-hydroxycryptopleurine (2). The structure of the new compound 2 was determined by spectroscopic methods. Compounds 1 and 2 potently inhibited the hypoxia-induced expression of a reporter gene under the control of a hypoxia response element (HRE) with IC50 values of 8.7 and 48.1 nM, respectively. Furthermore, 1 and 2 suppressed the accumulation of HEF-1α protein in a dose-dependent manner, but not the HIF-1β protein and inhibited expression of vascular endothelial growth factor (VEGF) by hypoxia.

Original languageEnglish
Pages (from-to)1095-1097
Number of pages3
JournalJournal of Natural Products
Volume69
Issue number7
DOIs
Publication statusPublished - 2006 Jul
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

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