Phenolic compounds from Pueraria lobata protect PC12 cells against Aβ-induced toxicity

Yun Hyeok Choi, Seong Su Hong, Yu Su Shin, Bang Yeon Hwang, So Young Park, Dongho Lee

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Bioassay-guided fractionation of the EtOAc-soluble extract of Pueraria lobata based on the inhibition of Aβ-induced toxicity in PC12 cells resulted in the isolation of four known active compounds, genistein (8), biochanin A (9), sissotrin (10), and puerol B (11). Of these, genistein (8) and biochanin A (9) exhibited potent neuroprotective effects with ED50 values of 33.7 and 27.8 μM, respectively. In addition, a new coumestan, 2-(α,α-dimethylallyl)coumestrol (1) was isolated and characterized, but proved to be inactive, as were additional seven known compounds. The structure of new compound 1 was determined using spectroscopic techniques.

Original languageEnglish
Pages (from-to)1651-1654
Number of pages4
JournalArchives of Pharmacal Research
Volume33
Issue number10
DOIs
Publication statusPublished - 2010 Oct 1

Fingerprint

Pueraria
Genistein
PC12 Cells
Toxicity
Coumestrol
Bioassay
Neuroprotective Agents
Fractionation
Biological Assay
biochanin A

Keywords

  • 2-(α,α-dimethylallyl)coumestrol
  • Beta-amyloid
  • Fabaceae
  • Isoflavone
  • Pueraria lobata

ASJC Scopus subject areas

  • Drug Discovery
  • Molecular Medicine
  • Organic Chemistry

Cite this

Phenolic compounds from Pueraria lobata protect PC12 cells against Aβ-induced toxicity. / Choi, Yun Hyeok; Hong, Seong Su; Shin, Yu Su; Hwang, Bang Yeon; Park, So Young; Lee, Dongho.

In: Archives of Pharmacal Research, Vol. 33, No. 10, 01.10.2010, p. 1651-1654.

Research output: Contribution to journalArticle

Choi, Yun Hyeok ; Hong, Seong Su ; Shin, Yu Su ; Hwang, Bang Yeon ; Park, So Young ; Lee, Dongho. / Phenolic compounds from Pueraria lobata protect PC12 cells against Aβ-induced toxicity. In: Archives of Pharmacal Research. 2010 ; Vol. 33, No. 10. pp. 1651-1654.
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