Phenolic compounds: Strong inhibitors derived from lignocellulosic hydrolysate for 2,3-butanediol production by Enterobacter aerogenes

Sang Jun Lee, Ju Hun Lee, Xiaoguang Yang, Sung Bong Kim, Ja Hyun Lee, Hah Young Yoo, Chulhwan Park, Seung Wook Kim

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Lignocellulosic biomass are attractive feedstocks for 2,3-butanediol production due to their abundant supply and low price. During the hydrolysis of lignocellulosic biomass, various byproducts are formed and their effects on 2,3-butanediol production were not sufficiently studied compared to ethanol production. Therefore, the effects of compounds derived from lignocellulosic biomass (weak acids, furan derivatives and phenolics) on the cell growth, the 2,3-butanediol production and the enzymes activity involved in 2,3-butanediol production were evaluated using Enterobacter aerogenes ATCC 29007. The phenolic compounds showed the most toxic effects on cell growth, 2,3-butanediol production and enzyme activity, followed by furan derivatives and weak acids. The significant effects were not observed in the presence of acetic acid and formic acid. Also, feasibility of 2,3-butanediol production from lignocellulosic biomass was evaluated using Miscanthus as a feedstock. In the fermentation of Miscanthus hydrolysate, 11.00 g/L of 2,3-butanediol was obtained from 34.62 g/L of reducing sugar. However, 2,3-butanediol was not produced when the concentration of total phenolic compounds in the hydrolysate increased to more than 1.5 g/L. The present study provides useful information to develop strategies for biological production of 2,3-butanediol and to establish biorefinery for biochemicals from lignocellulosic biomass.

Original languageEnglish
Pages (from-to)1920-1928
Number of pages9
JournalBiotechnology Journal
Volume10
Issue number12
DOIs
Publication statusPublished - 2015 Dec 1

Keywords

  • 2,3-butanediol
  • Enterobacter aerogenes
  • Inhibitory effects
  • Lignocellulosic hydrolysate
  • Miscanthus

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Molecular Medicine

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