Phenotypic Discovery of an Antivirulence Agent against Vibrio vulnificus via Modulation of Quorum-Sensing Regulator SmcR

Seung Min Kim, Jongmin Park, Myun Soo Kim, Heebum Song, Ala Jo, Hankum Park, Tae Sung Kim, Sang Ho Choi, Seung Bum Park

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

An antivirulence agent against Vibrio vulnificus named quoromycin (QM) was discovered by a phenotype-based elastase inhibitor screening. Using the fluorescence difference in two-dimensional gel electrophoresis (FITGE) approach, SmcR, a quorum-sensing master regulator and homologue of LuxR, was identified as the target protein of QM. We confirmed that the direct binding of QM to SmcR inhibits the quorum-sensing signaling pathway by controlling the DNA-binding affinity of SmcR and thus effectively alleviates the virulence of V. vulnificus in vitro and in vivo. QM can be regarded as a novel antivirulence agent for the treatment of V. vulnificus infection.

Original languageEnglish
Pages (from-to)3076-3082
Number of pages7
JournalACS Infectious Diseases
Volume6
Issue number11
DOIs
Publication statusPublished - 2020 Nov 13

Keywords

  • SmcR
  • Vibrio vulnificus
  • antivirulence agent
  • quorum sensing
  • target identification

ASJC Scopus subject areas

  • Infectious Diseases

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