@article{5a43240de1a24a2f8bdbef7296d78048,
title = "Phenotypic Discovery of an Antivirulence Agent against Vibrio vulnificus via Modulation of Quorum-Sensing Regulator SmcR",
abstract = "An antivirulence agent against Vibrio vulnificus named quoromycin (QM) was discovered by a phenotype-based elastase inhibitor screening. Using the fluorescence difference in two-dimensional gel electrophoresis (FITGE) approach, SmcR, a quorum-sensing master regulator and homologue of LuxR, was identified as the target protein of QM. We confirmed that the direct binding of QM to SmcR inhibits the quorum-sensing signaling pathway by controlling the DNA-binding affinity of SmcR and thus effectively alleviates the virulence of V. vulnificus in vitro and in vivo. QM can be regarded as a novel antivirulence agent for the treatment of V. vulnificus infection. ",
keywords = "SmcR, Vibrio vulnificus, antivirulence agent, quorum sensing, target identification",
author = "Kim, {Seung Min} and Jongmin Park and Kim, {Myun Soo} and Heebum Song and Ala Jo and Hankum Park and Kim, {Tae Sung} and Choi, {Sang Ho} and Park, {Seung Bum}",
note = "Funding Information: This work was supported by the Creative Research Initiative Grant (2014R1A3A2030423), the Bio & Medical Technology Development Program (2012M3A9C4048780), and the Midcareer Researcher Program (2012R1A2A1A03009679) through the National Research Foundation of Korea (NRF) funded by the Korean Government (Ministry of Science & ICT). This work was also supported by a grant (20162MFDS142) from the Ministry of Food and Drug Safety in 2020 and a 2018 Research Grant from Kangwon National University. We thank the Korea Chemical Bank ( http://www.chembank.org ) of the Korea Research Institute of Chemical Technology for generously providing the small-molecule libraries. Publisher Copyright: {\textcopyright} ",
year = "2020",
month = nov,
day = "13",
doi = "10.1021/acsinfecdis.0c00587",
language = "English",
volume = "6",
pages = "3076--3082",
journal = "ACS Infectious Diseases",
issn = "2373-8227",
publisher = "American Chemical Society",
number = "11",
}
