Pheophorbide a from Capsosiphon fulvescens Inhibits Advanced Glycation End Products Mediated Endothelial Dysfunction

Chung Oui Hong, Mi Hyun Nam, Ji Sun Oh, Jin Won Lee, Cheong Tae Kim, Kwon Woo Park, Dongho Lee, Kwang Won Lee

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

During hyperglycemia, the first step toward the formation of advanced glycation end products is the nonenzymatic glycation between the carbonyl group of a sugar and the primary amino group of a protein. Advanced glycation end products are then produced through more complex reactions. Reactive oxygen species derived from advanced glycation end products may play a key role in inflammation of the endothelium, leading to the complications seen in diabetes. Glycolaldehyde-induced advanced glycation end products have been reported to express proinflammatory cytokines, such as tumor necrosis factor-α and interleukin-1β. This study focused on Capsosiphon fulvescens, a Capsosiphonaceae type of green algae that has shown potential as a functional food material. Pheophorbide a, an anti-glycation compound, was isolated from C. fulvescens by extraction using a mixture of ethanol and water, followed by column fractionation of the resulting extract. The compound separated from C. fulvescens was identified by means of high-performance liquid chromatography combined with mass spectrometry. Pheophorbide a showed scavenging activity of the intracellular reactive oxygen species as well as monocyte adhesiveness inhibitory activity on the human myelomonocytic cell line (THP-1) and human umbilical vein endothelial cells cocultivation system. The mRNA levels of inflammation-related genes such as monocyte chemoattractant protein-1 and interleukin-6 were significantly decreased by pheophorbide a, and advanced glycation end products-stimulated tumor necrosis factor-α and interleukin-1β were downregulated as well. These results indicate that pheophorbide a has significant reactive oxygen species-scavenging activity, monocyte adhesive inhibitory activity, and downregulatory activity of cytokines related to inflammation affecting the endothelium. Pheophorbide a could therefore be a promising candidate for modulating endothelial cell dysfunction.

Original languageEnglish
Pages (from-to)46-57
Number of pages12
JournalPlanta Medica
Volume82
Issue number1-2
DOIs
Publication statusPublished - 2015 Aug 26

Fingerprint

Advanced Glycosylation End Products
Reactive Oxygen Species
Scavenging
Endothelial cells
Inflammation
Interleukin-1
Endothelium
Monocytes
Tumor Necrosis Factor-alpha
Cytokines
Amino Sugars
Adhesiveness
Functional Food
Chlorophyta
Chemokine CCL2
Distillation columns
Human Umbilical Vein Endothelial Cells
High performance liquid chromatography
Algae
Fractionation

Keywords

  • advanced glycation end product
  • Capsosiphon fulvescens
  • Capsosiphonaceae
  • endothelial cell dysfunction
  • pheophorbide a
  • proinflammatory cytokine

ASJC Scopus subject areas

  • Drug Discovery
  • Pharmacology
  • Pharmaceutical Science
  • Analytical Chemistry
  • Organic Chemistry
  • Molecular Medicine
  • Complementary and alternative medicine

Cite this

Pheophorbide a from Capsosiphon fulvescens Inhibits Advanced Glycation End Products Mediated Endothelial Dysfunction. / Hong, Chung Oui; Nam, Mi Hyun; Oh, Ji Sun; Lee, Jin Won; Kim, Cheong Tae; Park, Kwon Woo; Lee, Dongho; Lee, Kwang Won.

In: Planta Medica, Vol. 82, No. 1-2, 26.08.2015, p. 46-57.

Research output: Contribution to journalArticle

Hong, Chung Oui ; Nam, Mi Hyun ; Oh, Ji Sun ; Lee, Jin Won ; Kim, Cheong Tae ; Park, Kwon Woo ; Lee, Dongho ; Lee, Kwang Won. / Pheophorbide a from Capsosiphon fulvescens Inhibits Advanced Glycation End Products Mediated Endothelial Dysfunction. In: Planta Medica. 2015 ; Vol. 82, No. 1-2. pp. 46-57.
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AU - Lee, Kwang Won

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