Phorbaketal A stimulates osteoblast differentiation through TAZ mediated Runx2 activation

Mi Ran Byun; A. Rum Kim; Jun Ha Hwang; Mi Kyung Sung; Yeon Kyung Lee; Buyng Su Hwang; Jung Rae Rho; Eun Sook Hwang; Jeong Ho Hong

doi:10.1016/j.febslet.2012.03.008

Phorbaketal A stimulates osteoblast differentiation through TAZ mediated Runx2 activation

Mi Ran Byun, A. Rum Kim, Jun Ha Hwang, Mi Kyung Sung, Yeon Kyung Lee, Buyng Su Hwang, Jung Rae Rho, Eun Sook Hwang, Jeong Ho Hong

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

Osteoporosis arises from an imbalance between osteoblastic bone formation and osteoclastic bone resorption. In this study, we screened molecules from marine natural products that stimulate osteoblast differentiation. We found that phorbaketal A significantly stimulates osteoblast differentiation in mesenchymal cells. Increased interaction of TAZ and Runx2 stimulated phorbaketal A-induced expression of osteoblastic marker genes. The activation of ERK was important for the stimulation of differentiation because an inhibitor of ERK blocked phorbaketal A-induced osteogenic differentiation. Taken together, the results showed that phorbaketal A stimulates TAZ-mediated osteoblast differentiation through the activation of ERK. Structured summary of protein interactions: TAZ physically interacts with RUNX2 by pull down (View interaction).

Original language	English
Pages (from-to)	1086-1092
Number of pages	7
Journal	FEBS Letters
Volume	586
Issue number	8
DOIs	https://doi.org/10.1016/j.febslet.2012.03.008
Publication status	Published - 2012 Apr 24

Keywords

Differentiation
ERK
Osteoblast
Phorbaketal A
TAZ

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.febslet.2012.03.008

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title = "Phorbaketal A stimulates osteoblast differentiation through TAZ mediated Runx2 activation",

abstract = "Osteoporosis arises from an imbalance between osteoblastic bone formation and osteoclastic bone resorption. In this study, we screened molecules from marine natural products that stimulate osteoblast differentiation. We found that phorbaketal A significantly stimulates osteoblast differentiation in mesenchymal cells. Increased interaction of TAZ and Runx2 stimulated phorbaketal A-induced expression of osteoblastic marker genes. The activation of ERK was important for the stimulation of differentiation because an inhibitor of ERK blocked phorbaketal A-induced osteogenic differentiation. Taken together, the results showed that phorbaketal A stimulates TAZ-mediated osteoblast differentiation through the activation of ERK. Structured summary of protein interactions: TAZ physically interacts with RUNX2 by pull down (View interaction).",

keywords = "Differentiation, ERK, Osteoblast, Phorbaketal A, TAZ",

author = "Byun, {Mi Ran} and Kim, {A. Rum} and Hwang, {Jun Ha} and Sung, {Mi Kyung} and Lee, {Yeon Kyung} and Hwang, {Buyng Su} and Rho, {Jung Rae} and Hwang, {Eun Sook} and Hong, {Jeong Ho}",

note = "Funding Information: This work was supported by basic science research programs (2007-0053654 and 2011-0022926) and the BRL program (2009-0001197) through the National Research Foundation of Korea (NRF) and a Grant from the Marine Biotechnology Program funded by the Land, Transport and Maritime Affairs, Republic of Korea. ",

year = "2012",

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doi = "10.1016/j.febslet.2012.03.008",

language = "English",

volume = "586",

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journal = "FEBS Letters",

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T1 - Phorbaketal A stimulates osteoblast differentiation through TAZ mediated Runx2 activation

AU - Byun, Mi Ran

AU - Kim, A. Rum

AU - Hwang, Jun Ha

AU - Sung, Mi Kyung

AU - Lee, Yeon Kyung

AU - Hwang, Buyng Su

AU - Rho, Jung Rae

AU - Hwang, Eun Sook

AU - Hong, Jeong Ho

N1 - Funding Information: This work was supported by basic science research programs (2007-0053654 and 2011-0022926) and the BRL program (2009-0001197) through the National Research Foundation of Korea (NRF) and a Grant from the Marine Biotechnology Program funded by the Land, Transport and Maritime Affairs, Republic of Korea.

PY - 2012/4/24

Y1 - 2012/4/24

N2 - Osteoporosis arises from an imbalance between osteoblastic bone formation and osteoclastic bone resorption. In this study, we screened molecules from marine natural products that stimulate osteoblast differentiation. We found that phorbaketal A significantly stimulates osteoblast differentiation in mesenchymal cells. Increased interaction of TAZ and Runx2 stimulated phorbaketal A-induced expression of osteoblastic marker genes. The activation of ERK was important for the stimulation of differentiation because an inhibitor of ERK blocked phorbaketal A-induced osteogenic differentiation. Taken together, the results showed that phorbaketal A stimulates TAZ-mediated osteoblast differentiation through the activation of ERK. Structured summary of protein interactions: TAZ physically interacts with RUNX2 by pull down (View interaction).

AB - Osteoporosis arises from an imbalance between osteoblastic bone formation and osteoclastic bone resorption. In this study, we screened molecules from marine natural products that stimulate osteoblast differentiation. We found that phorbaketal A significantly stimulates osteoblast differentiation in mesenchymal cells. Increased interaction of TAZ and Runx2 stimulated phorbaketal A-induced expression of osteoblastic marker genes. The activation of ERK was important for the stimulation of differentiation because an inhibitor of ERK blocked phorbaketal A-induced osteogenic differentiation. Taken together, the results showed that phorbaketal A stimulates TAZ-mediated osteoblast differentiation through the activation of ERK. Structured summary of protein interactions: TAZ physically interacts with RUNX2 by pull down (View interaction).

KW - Differentiation

KW - ERK

KW - Osteoblast

KW - Phorbaketal A

KW - TAZ

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DO - 10.1016/j.febslet.2012.03.008

M3 - Article

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AN - SCOPUS:84862811661

SN - 0014-5793

VL - 586

SP - 1086

EP - 1092

JO - FEBS Letters

JF - FEBS Letters

IS - 8

ER -

Phorbaketal A stimulates osteoblast differentiation through TAZ mediated Runx2 activation

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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