Phosphatase and actin regulator 4 is associated with intermediate filaments in adult neural stem cells and their progenitor astrocytes

Hyo M in Cho, Joo Y eon Kim, Hyun Kim, Woong Sun

Research output: Contribution to journalArticle

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Abstract

Phosphatase and actin regulator 4 (Phactr4) is a newly discovered protein that inhibits protein phosphatase 1 and shows actin-binding activity. We previously found that Phactr4 is expressed in the neurogenic niche in adult mice, although its precise subcellular localization and possible function in neural stem cells (NSCs) is not yet understood. Here, we show that Phactr4 formed punctiform clusters in the cytosol of subventricular zone-derived adult NSCs and their progeny in vitro. These Phactr4 signals were not associated with F-actin fibers but were closely associated with intermediate filaments such as nestin and glial fibrillary acidic protein (GFAP) fibers. Direct binding of Phactr4 with nestin and GFAP filaments was demonstrated using Duolink protein interaction analyses and immunoprecipitation assays. Interestingly, when nestin fibers were de-polymerized during the mitosis or by the phosphatase inhibitor, Phactr4 appeared to be dissociated from nestin, suggesting that their protein interaction is regulated by the protein phosphorylation. These results suggest that Phactr4 forms functional associations with intermediate filament networks in adult NSCs.

Original languageEnglish
Pages (from-to)411-419
Number of pages9
JournalHistochemistry and Cell Biology
Volume142
Issue number4
DOIs
Publication statusPublished - 2014 Oct 1

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Adult Stem Cells
Neural Stem Cells
Intermediate Filaments
Phosphoric Monoester Hydrolases
Astrocytes
Actins
Nestin
Glial Fibrillary Acidic Protein
Proteins
Protein Phosphatase 1
Lateral Ventricles
Immunoprecipitation
Mitosis
Cytosol
Phosphorylation

ASJC Scopus subject areas

  • Medicine(all)

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Phosphatase and actin regulator 4 is associated with intermediate filaments in adult neural stem cells and their progenitor astrocytes. / Cho, Hyo M in; Kim, Joo Y eon; Kim, Hyun; Sun, Woong.

In: Histochemistry and Cell Biology, Vol. 142, No. 4, 01.10.2014, p. 411-419.

Research output: Contribution to journalArticle

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