TY - JOUR
T1 - Phospholipase A1-catalyzed synthesis of phospholipids enriched in n - 3 polyunsaturated fatty acid residues
AU - Kim, In Hwan
AU - Garcia, Hugo S.
AU - Hill, Charles Graham
N1 - Funding Information:
This work was supported by the University of Wisconsin Sea Grant Institute under grants from the National Sea Grant College Program, National Oceanic and Atmospheric Administration, US Department of Commerce, and from the State of Wisconsin (Federal grant number NA 46 RG 0481, project number R/AQ-34). Additional support was provided by National Science Foundation Grant BES-077524. We also thank Mr. Iswandi Jarto for his contributions to the experimental work.
PY - 2007/4/3
Y1 - 2007/4/3
N2 - Phosphatidylcholine (PC) was successfully modified by phospholipase A1-catalyzed acidolysis with the fatty acids obtained by saponification of fish oil. The resulting phosphatidylcholine contains significant levels of eicosapentaenoic (EPA; C20:5), docosapentaenoic (DPA; C22:5), and docosahexaenoic (DHA; C22:6) acid residues. Modification of the PC was accomplished in a solvent-free system using phospholipase A1 from Thermomyces lanuginosus/Fusarium oxysporum as the biocatalyst. The effects of variations in the reaction parameters, namely, reaction time, enzyme loading, temperature, and vacuum on the time course of the reaction were investigated. After only 6 h of reaction at 55 °C, 21.0 mol% n - 3 polyunsaturated fatty acids (PUFA) (sum of EPA, DPA, and DHA) was incorporated into PC at a loading of the enzyme solution of 10% of the total weight of substrates. As the reaction progressed, incorporation of n - 3 PUFA reached a maximum of 28.0 mol% at 24 h. Reaction times longer than 6 h led to higher incorporation of n - 3 PUFA but were associated with significant decreases in the yield of PC. Even though application of a vacuum produced a higher yield of PC, there was a parallel decrease in the extent of incorporation of n - 3 PUFA into PC.
AB - Phosphatidylcholine (PC) was successfully modified by phospholipase A1-catalyzed acidolysis with the fatty acids obtained by saponification of fish oil. The resulting phosphatidylcholine contains significant levels of eicosapentaenoic (EPA; C20:5), docosapentaenoic (DPA; C22:5), and docosahexaenoic (DHA; C22:6) acid residues. Modification of the PC was accomplished in a solvent-free system using phospholipase A1 from Thermomyces lanuginosus/Fusarium oxysporum as the biocatalyst. The effects of variations in the reaction parameters, namely, reaction time, enzyme loading, temperature, and vacuum on the time course of the reaction were investigated. After only 6 h of reaction at 55 °C, 21.0 mol% n - 3 polyunsaturated fatty acids (PUFA) (sum of EPA, DPA, and DHA) was incorporated into PC at a loading of the enzyme solution of 10% of the total weight of substrates. As the reaction progressed, incorporation of n - 3 PUFA reached a maximum of 28.0 mol% at 24 h. Reaction times longer than 6 h led to higher incorporation of n - 3 PUFA but were associated with significant decreases in the yield of PC. Even though application of a vacuum produced a higher yield of PC, there was a parallel decrease in the extent of incorporation of n - 3 PUFA into PC.
KW - Acidolysis
KW - Fish oil
KW - Phosphatidylcholine
KW - Phospholipase A
KW - n - 3 polyunsaturated fatty acid (PUFA)
UR - http://www.scopus.com/inward/record.url?scp=33847696154&partnerID=8YFLogxK
U2 - 10.1016/j.enzmictec.2006.08.018
DO - 10.1016/j.enzmictec.2006.08.018
M3 - Article
AN - SCOPUS:33847696154
VL - 40
SP - 1130
EP - 1135
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
SN - 0141-0229
IS - 5
ER -