Abstract
Phosphatidylcholine (PC) was successfully modified by phospholipase A1-catalyzed acidolysis with the fatty acids obtained by saponification of fish oil. The resulting phosphatidylcholine contains significant levels of eicosapentaenoic (EPA; C20:5), docosapentaenoic (DPA; C22:5), and docosahexaenoic (DHA; C22:6) acid residues. Modification of the PC was accomplished in a solvent-free system using phospholipase A1 from Thermomyces lanuginosus/Fusarium oxysporum as the biocatalyst. The effects of variations in the reaction parameters, namely, reaction time, enzyme loading, temperature, and vacuum on the time course of the reaction were investigated. After only 6 h of reaction at 55 °C, 21.0 mol% n - 3 polyunsaturated fatty acids (PUFA) (sum of EPA, DPA, and DHA) was incorporated into PC at a loading of the enzyme solution of 10% of the total weight of substrates. As the reaction progressed, incorporation of n - 3 PUFA reached a maximum of 28.0 mol% at 24 h. Reaction times longer than 6 h led to higher incorporation of n - 3 PUFA but were associated with significant decreases in the yield of PC. Even though application of a vacuum produced a higher yield of PC, there was a parallel decrease in the extent of incorporation of n - 3 PUFA into PC.
| Original language | English |
|---|---|
| Pages (from-to) | 1130-1135 |
| Number of pages | 6 |
| Journal | Enzyme and Microbial Technology |
| Volume | 40 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2007 Apr 3 |
Fingerprint
Keywords
- Acidolysis
- Fish oil
- n - 3 polyunsaturated fatty acid (PUFA)
- Phosphatidylcholine
- Phospholipase A
ASJC Scopus subject areas
- Biochemistry
- Biotechnology
- Applied Microbiology and Biotechnology
Cite this
Phospholipase A1-catalyzed synthesis of phospholipids enriched in n - 3 polyunsaturated fatty acid residues. / Kim, In-Hwan; Garcia, Hugo S.; Hill, Charles Graham.
In: Enzyme and Microbial Technology, Vol. 40, No. 5, 03.04.2007, p. 1130-1135.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phospholipase A1-catalyzed synthesis of phospholipids enriched in n - 3 polyunsaturated fatty acid residues
AU - Kim, In-Hwan
AU - Garcia, Hugo S.
AU - Hill, Charles Graham
PY - 2007/4/3
Y1 - 2007/4/3
N2 - Phosphatidylcholine (PC) was successfully modified by phospholipase A1-catalyzed acidolysis with the fatty acids obtained by saponification of fish oil. The resulting phosphatidylcholine contains significant levels of eicosapentaenoic (EPA; C20:5), docosapentaenoic (DPA; C22:5), and docosahexaenoic (DHA; C22:6) acid residues. Modification of the PC was accomplished in a solvent-free system using phospholipase A1 from Thermomyces lanuginosus/Fusarium oxysporum as the biocatalyst. The effects of variations in the reaction parameters, namely, reaction time, enzyme loading, temperature, and vacuum on the time course of the reaction were investigated. After only 6 h of reaction at 55 °C, 21.0 mol% n - 3 polyunsaturated fatty acids (PUFA) (sum of EPA, DPA, and DHA) was incorporated into PC at a loading of the enzyme solution of 10% of the total weight of substrates. As the reaction progressed, incorporation of n - 3 PUFA reached a maximum of 28.0 mol% at 24 h. Reaction times longer than 6 h led to higher incorporation of n - 3 PUFA but were associated with significant decreases in the yield of PC. Even though application of a vacuum produced a higher yield of PC, there was a parallel decrease in the extent of incorporation of n - 3 PUFA into PC.
AB - Phosphatidylcholine (PC) was successfully modified by phospholipase A1-catalyzed acidolysis with the fatty acids obtained by saponification of fish oil. The resulting phosphatidylcholine contains significant levels of eicosapentaenoic (EPA; C20:5), docosapentaenoic (DPA; C22:5), and docosahexaenoic (DHA; C22:6) acid residues. Modification of the PC was accomplished in a solvent-free system using phospholipase A1 from Thermomyces lanuginosus/Fusarium oxysporum as the biocatalyst. The effects of variations in the reaction parameters, namely, reaction time, enzyme loading, temperature, and vacuum on the time course of the reaction were investigated. After only 6 h of reaction at 55 °C, 21.0 mol% n - 3 polyunsaturated fatty acids (PUFA) (sum of EPA, DPA, and DHA) was incorporated into PC at a loading of the enzyme solution of 10% of the total weight of substrates. As the reaction progressed, incorporation of n - 3 PUFA reached a maximum of 28.0 mol% at 24 h. Reaction times longer than 6 h led to higher incorporation of n - 3 PUFA but were associated with significant decreases in the yield of PC. Even though application of a vacuum produced a higher yield of PC, there was a parallel decrease in the extent of incorporation of n - 3 PUFA into PC.
KW - Acidolysis
KW - Fish oil
KW - n - 3 polyunsaturated fatty acid (PUFA)
KW - Phosphatidylcholine
KW - Phospholipase A
UR - http://www.scopus.com/inward/record.url?scp=33847696154&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33847696154&partnerID=8YFLogxK
U2 - 10.1016/j.enzmictec.2006.08.018
DO - 10.1016/j.enzmictec.2006.08.018
M3 - Article
AN - SCOPUS:33847696154
VL - 40
SP - 1130
EP - 1135
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
SN - 0141-0229
IS - 5
ER -