Phospholipase A1-catalyzed synthesis of phospholipids enriched in n - 3 polyunsaturated fatty acid residues

In Hwan Kim, Hugo S. Garcia, Charles Graham Hill

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Phosphatidylcholine (PC) was successfully modified by phospholipase A1-catalyzed acidolysis with the fatty acids obtained by saponification of fish oil. The resulting phosphatidylcholine contains significant levels of eicosapentaenoic (EPA; C20:5), docosapentaenoic (DPA; C22:5), and docosahexaenoic (DHA; C22:6) acid residues. Modification of the PC was accomplished in a solvent-free system using phospholipase A1 from Thermomyces lanuginosus/Fusarium oxysporum as the biocatalyst. The effects of variations in the reaction parameters, namely, reaction time, enzyme loading, temperature, and vacuum on the time course of the reaction were investigated. After only 6 h of reaction at 55 °C, 21.0 mol% n - 3 polyunsaturated fatty acids (PUFA) (sum of EPA, DPA, and DHA) was incorporated into PC at a loading of the enzyme solution of 10% of the total weight of substrates. As the reaction progressed, incorporation of n - 3 PUFA reached a maximum of 28.0 mol% at 24 h. Reaction times longer than 6 h led to higher incorporation of n - 3 PUFA but were associated with significant decreases in the yield of PC. Even though application of a vacuum produced a higher yield of PC, there was a parallel decrease in the extent of incorporation of n - 3 PUFA into PC.

Original languageEnglish
Pages (from-to)1130-1135
Number of pages6
JournalEnzyme and Microbial Technology
Volume40
Issue number5
DOIs
Publication statusPublished - 2007 Apr 3

Keywords

  • Acidolysis
  • Fish oil
  • Phosphatidylcholine
  • Phospholipase A
  • n - 3 polyunsaturated fatty acid (PUFA)

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

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