Phospholipase D is not involved in Rho A-mediated activation of stress fiber formation

Sun Hee Leem, Incheol Shin, Soo Mi Kweon, Seung Il Kim, Jae-Hong Kim, Kwon Soo Ha

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In order to investigate the role of a small GTP-binding protein RhoA in lysophosphatidic acid (LPA)-induced stress fiber formation, C3 ADP-ribosyltransferase was prepared by expressing in E. coli and then applied to Rat-2 fibroblasts. C3 transferase isolated from E. coli was as effective as the toxin from Clostridium botulinum in ADP-ribosylation of RhoA. Incubation of the cells with C3 transferase for 2 days induced ADP-ribosylation of RhoA by a dose-dependent manner, with a sub-maximal induction at 25 μg/ml. As expected, LPA-induced stress fiber formation was completely blocked by pre-incubation with C3 transferase for 2 days. However, exogenously added C3 transferase had no significant effect on the formation of phosphatidylethanol by LPA. These results suggested that phospholipase D was not activated by RhoA in the LPA-induced stress fiber formation.

Original languageEnglish
Pages (from-to)337-341
Number of pages5
JournalJournal of Biochemistry and Molecular Biology
Volume30
Issue number5
Publication statusPublished - 1997 Sep 30
Externally publishedYes

Fingerprint

Phospholipase D
Stress Fibers
Transferases
Chemical activation
Fibers
Adenosine Diphosphate
Escherichia coli
rhoA GTP-Binding Protein
ADP Ribose Transferases
Botulinum Toxins
Fibroblasts
Rats
Cells
lysophosphatidic acid

Keywords

  • C3 transferase
  • Lysophosphatidic acid
  • Phospholipase D
  • RhoA
  • Stress fibers

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Phospholipase D is not involved in Rho A-mediated activation of stress fiber formation. / Leem, Sun Hee; Shin, Incheol; Kweon, Soo Mi; Kim, Seung Il; Kim, Jae-Hong; Ha, Kwon Soo.

In: Journal of Biochemistry and Molecular Biology, Vol. 30, No. 5, 30.09.1997, p. 337-341.

Research output: Contribution to journalArticle

Leem, Sun Hee ; Shin, Incheol ; Kweon, Soo Mi ; Kim, Seung Il ; Kim, Jae-Hong ; Ha, Kwon Soo. / Phospholipase D is not involved in Rho A-mediated activation of stress fiber formation. In: Journal of Biochemistry and Molecular Biology. 1997 ; Vol. 30, No. 5. pp. 337-341.
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AU - Ha, Kwon Soo

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