Phosphorylation of d-allose by hexokinase involved in regulation of OsABF1 expression for growth inhibition in Oryza sativa L.

Takeshi Fukumoto, Akihito Kano, Kouhei Ohtani, Megumi Inoue, Akihide Yoshihara, Ken Izumori, Shigeyuki Tajima, Yoshio Shigematsu, Keiji Tanaka, Takeo Ohkouchi, Yutaka Ishida, Yoko Nishizawa, Yasuomi Tada, Kazuya Ichimura, Kenji Gomi, Sang-Dong Yoo, Jen Sheen, Kazuya Akimitsu

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We previously reported that a rare sugar d-allose, which is the d-glucose epimer at C3, inhibits the gibberellin-dependent responses such as elongation of the second leaf sheath and induction of α-amylase in embryo-less half seeds in rice (Fukumoto et al. 2011). d-Allose suppresses expressions of gibberellin-responsive genes downstream of SLR1 protein in the gibberellin-signaling through hexokinase (HXK)-dependent pathway. In this study, we discovered that d-allose induced expression of ABA-related genes including OsNCED1-3 and OsABA8ox1-3 in rice. Interestingly, d-allose also up-regulated expression of OsABF1, encoding a conserved bZIP transcription factor in ABA signaling, in rice. The d-allose-induced expression of OsABF1 was diminished by a hexokinase inhibitor, d-mannoheptulose (MNH). Consistently, d-allose also inhibited Arabidopsis growth, but failed to trigger growth retardation in the glucose-insensitive2 (gin2) mutant, which is a loss-of-function mutant of the glucose sensor AtHXK1. d-Allose activated AtABI5 expression in transgenic gin2 over-expressing wild-type AtHXK1 but not in gin2 over-expressing the catalytic mutant AtHXK1S177A, indicating that the d-allose phosphorylation by HXK to d-allose 6-phosphate (A6P) is the first step for the up-regulation of AtABI5 gene expression as well as d-allose-induced growth inhibition. Moreover, overexpression of OsABF1 showed increased sensitivity to d-allose in rice. These findings indicated that the phosphorylation of d-allose at C6 by hexokinase is essential and OsABF1 is involved in the signal transduction for d-allose-induced growth inhibition.

Original languageEnglish
Pages (from-to)1379-1391
Number of pages13
JournalPlanta
Volume237
Issue number5
DOIs
Publication statusPublished - 2013 Feb 11
Externally publishedYes

Fingerprint

allose
Hexokinase
hexokinase
growth retardation
phosphorylation
Oryza sativa
Phosphorylation
Growth
Gibberellins
Glucose
glucose
gibberellins
rice
Oryza
mutants
Mannoheptulose
Basic-Leucine Zipper Transcription Factors
gene expression regulation
Gene Expression Regulation

Keywords

  • Abscisic acid
  • AtABI5
  • d-Allose
  • Hexokinase
  • OsABF1

ASJC Scopus subject areas

  • Plant Science
  • Genetics

Cite this

Fukumoto, T., Kano, A., Ohtani, K., Inoue, M., Yoshihara, A., Izumori, K., ... Akimitsu, K. (2013). Phosphorylation of d-allose by hexokinase involved in regulation of OsABF1 expression for growth inhibition in Oryza sativa L. Planta, 237(5), 1379-1391. https://doi.org/10.1007/s00425-013-1853-9

Phosphorylation of d-allose by hexokinase involved in regulation of OsABF1 expression for growth inhibition in Oryza sativa L. / Fukumoto, Takeshi; Kano, Akihito; Ohtani, Kouhei; Inoue, Megumi; Yoshihara, Akihide; Izumori, Ken; Tajima, Shigeyuki; Shigematsu, Yoshio; Tanaka, Keiji; Ohkouchi, Takeo; Ishida, Yutaka; Nishizawa, Yoko; Tada, Yasuomi; Ichimura, Kazuya; Gomi, Kenji; Yoo, Sang-Dong; Sheen, Jen; Akimitsu, Kazuya.

In: Planta, Vol. 237, No. 5, 11.02.2013, p. 1379-1391.

Research output: Contribution to journalArticle

Fukumoto, T, Kano, A, Ohtani, K, Inoue, M, Yoshihara, A, Izumori, K, Tajima, S, Shigematsu, Y, Tanaka, K, Ohkouchi, T, Ishida, Y, Nishizawa, Y, Tada, Y, Ichimura, K, Gomi, K, Yoo, S-D, Sheen, J & Akimitsu, K 2013, 'Phosphorylation of d-allose by hexokinase involved in regulation of OsABF1 expression for growth inhibition in Oryza sativa L.', Planta, vol. 237, no. 5, pp. 1379-1391. https://doi.org/10.1007/s00425-013-1853-9
Fukumoto, Takeshi ; Kano, Akihito ; Ohtani, Kouhei ; Inoue, Megumi ; Yoshihara, Akihide ; Izumori, Ken ; Tajima, Shigeyuki ; Shigematsu, Yoshio ; Tanaka, Keiji ; Ohkouchi, Takeo ; Ishida, Yutaka ; Nishizawa, Yoko ; Tada, Yasuomi ; Ichimura, Kazuya ; Gomi, Kenji ; Yoo, Sang-Dong ; Sheen, Jen ; Akimitsu, Kazuya. / Phosphorylation of d-allose by hexokinase involved in regulation of OsABF1 expression for growth inhibition in Oryza sativa L. In: Planta. 2013 ; Vol. 237, No. 5. pp. 1379-1391.
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AU - Inoue, Megumi

AU - Yoshihara, Akihide

AU - Izumori, Ken

AU - Tajima, Shigeyuki

AU - Shigematsu, Yoshio

AU - Tanaka, Keiji

AU - Ohkouchi, Takeo

AU - Ishida, Yutaka

AU - Nishizawa, Yoko

AU - Tada, Yasuomi

AU - Ichimura, Kazuya

AU - Gomi, Kenji

AU - Yoo, Sang-Dong

AU - Sheen, Jen

AU - Akimitsu, Kazuya

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