Phosphorylation of peptides derived from isolated soybean protein: Effects on calcium binding, solubility and influx into Caco-2 cells

Shin Hee Lee, Jung Ik Yang, Suck Min Hong, Dae Hyun Hahm, Shin Young Lee, Il Hwan Kim, Sang-Yun Choi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Calcium homeostasis in the human body is maintained primarily via the absorption of calcium through the intestine. In order to maintain an efficient absorption of calcium with minimal calcium loss due to the formation of calcium phosphate precipitates in the small intestinal lumen, we developed a calcium-binding mediator using peptides derived from isolated soybean protein (ISP). ISP was modified via tryptic digestion and chemical phosphorylation using sodium trimetaphosphate, thereby generating soybean phosphopeptides (SPP), and this was followed by conducting a binding reaction with calcium chloride. We have established an optimized procedure and reaction conditions for maximal phosphorylation and calcium binding. Consequently, the phosphorylation of soybean peptides resulted in considerable improvement in their calcium binding activities. Next, we demonstrated that SPP was able to render calcium ions resistant to precipitate formation with inorganic phosphates, which suggested the enhancement of calcium bioavailability. Finally, we noted that the addition of calcium-bound SPP induced an increase in cytosolic calcium concentration in the intestinal Caco-2 cells, due to an influx of calcium. These findings provide a new basis by which we may assess the possibility that SPP, as a potent calcium carrier, can be utilized in the prevention of poor absorption of dietary calcium in animals.

Original languageEnglish
Pages (from-to)121-128
Number of pages8
JournalBioFactors
Volume23
Issue number3
DOIs
Publication statusPublished - 2005 Dec 1

Fingerprint

Phosphorylation
Soybean Proteins
Caco-2 Cells
Solubility
Calcium
Peptides
Phosphopeptides
Soybeans
Precipitates
Dietary Calcium
Calcium Chloride
Human Body
Biological Availability
Intestines
Digestion
Animals
Homeostasis

Keywords

  • Calcium
  • Peptides
  • Phosphorylation
  • Soybean protein

ASJC Scopus subject areas

  • Biochemistry

Cite this

Phosphorylation of peptides derived from isolated soybean protein : Effects on calcium binding, solubility and influx into Caco-2 cells. / Lee, Shin Hee; Yang, Jung Ik; Hong, Suck Min; Hahm, Dae Hyun; Lee, Shin Young; Kim, Il Hwan; Choi, Sang-Yun.

In: BioFactors, Vol. 23, No. 3, 01.12.2005, p. 121-128.

Research output: Contribution to journalArticle

Lee, Shin Hee ; Yang, Jung Ik ; Hong, Suck Min ; Hahm, Dae Hyun ; Lee, Shin Young ; Kim, Il Hwan ; Choi, Sang-Yun. / Phosphorylation of peptides derived from isolated soybean protein : Effects on calcium binding, solubility and influx into Caco-2 cells. In: BioFactors. 2005 ; Vol. 23, No. 3. pp. 121-128.
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