Phosphorylation of serine-10 of histone H3 shields modified lysine-9 selectively during mitosis

Young Sun Jeong, Sunwha Cho, Jung Sun Park, Yong Ko, Yong Kook Kang

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Post-translational modifications of histones play important roles in regulating chromatin dynamics and epigenetic inheritance during mitosis. The epigenetic significance and stability of histone H3-lysine 9 (H3K9) modifications have been well studied in interphase cells, whereas not as much in mitotic cells. Here, we inspected mitosis-coupled alterations in the global modifications of H3K9. Signals for H3K9 mono-, di-methylation and acetylation became invisible as cells entered mitosis in contrast to the pattern observed for H3-serine 10 phosphorylation (H3S10ph). Treatment with the aurora-B inhibitor ZM447439 or expression of the dominant negative mutant Aur-BK106R resulted in prometaphase chromosomes that lacked signals for H3S10ph but were positive for H3K9 modifications. Trimethylation was the sole K9 modification that remained consistently detectable throughout the cell cycle. This phenomenon was specific for H3K9-S10, as this pattern was not observed at H3K27-S28. Methylated H3K27 remained detectable throughout the cell cycle, despite phosphorylation of the adjacent H3S28. Contrastingly, our dot-blot experiment using synthetic peptides showed that phosphorylation of serine residue basically kept adjacent lysine from antibody access. Together, these results suggest that phosphorylation of serine residue occurs in a selective manner, being influenced by the types of modifications and the nature of neighboring lysine residues.

Original languageEnglish
Pages (from-to)181-192
Number of pages12
JournalGenes to Cells
Volume15
Issue number3
DOIs
Publication statusPublished - 2010 Mar 1

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Mitosis
Histones
Serine
Lysine
Phosphorylation
Epigenomics
Cell Cycle
S 10
Prometaphase
Interphase
Post Translational Protein Processing
Acetylation
Methylation
Chromatin
Chromosomes
Peptides
Antibodies

ASJC Scopus subject areas

  • Cell Biology
  • Genetics

Cite this

Phosphorylation of serine-10 of histone H3 shields modified lysine-9 selectively during mitosis. / Jeong, Young Sun; Cho, Sunwha; Park, Jung Sun; Ko, Yong; Kang, Yong Kook.

In: Genes to Cells, Vol. 15, No. 3, 01.03.2010, p. 181-192.

Research output: Contribution to journalArticle

Jeong, Young Sun ; Cho, Sunwha ; Park, Jung Sun ; Ko, Yong ; Kang, Yong Kook. / Phosphorylation of serine-10 of histone H3 shields modified lysine-9 selectively during mitosis. In: Genes to Cells. 2010 ; Vol. 15, No. 3. pp. 181-192.
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