Photo-affinity labeling (PAL) in chemical proteomics: A handy tool to investigate protein-protein interactions (PPIs)

Dhiraj P. Murale, Seong Cheol Hong, Md Mamunul Haque, Jun Seok Lee

Research output: Contribution to journalReview articlepeer-review

81 Citations (Scopus)

Abstract

Protein-protein interactions (PPIs) trigger a wide range of biological signaling pathways that are crucial for biomedical research and drug discovery. Various techniques have been used to study specific proteins, including affinity chromatography, activity-based probes, affinity-based probes and photo-affinity labeling (PAL). PAL has become one of the most powerful strategies to study PPIs. Traditional photocrosslinkers are used in PAL, including benzophenone, aryl azide, and diazirine. Upon photoirradiation, these photocrosslinkers (Pls) generate highly reactive species that react with adjacent molecules, resulting in a direct covalent modification. This review introduces recent examples of chemical proteomics study using PAL for PPIs.

Original languageEnglish
Article number14
JournalProteome Science
Volume15
Issue number1
DOIs
Publication statusPublished - 2017 Jun 24
Externally publishedYes

Keywords

  • Aryl azide
  • Benzophenone
  • Diazirine
  • Photo-affinity probe
  • Protein-protein interaction
  • Quantitative proteomics

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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