Photobiomodulation on human annulus fibrosus cells during the intervertebral disk degeneration

extracellular matrix-modifying enzymes

Min Ho Hwang, Kyoung Soo Kim, Chang Min Yoo, Jae Hee Shin, Hyo Geun Nam, Jin Su Jeong, Joo-Han Kim, Kwang Ho Lee, Hyuk Choi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Destruction of extracellular matrix (ECM) leads to degeneration of the intervertebral disk (IVD), which is a major contributor to many spine disorders. IVD degeneration is induced by pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β), which are secreted by immune cells, including macrophages and neutrophils. The cytokines modulate ECM-modifying enzymes such as matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in human annulus fibrosus (AF) cells. The resulting imbalance in catabolic and anabolic enzymes can cause generalized back, neck, and low back pain (LBP). Photobiomodulation (PBM) is known to regulate inflammatory responses and wound healing. The aim of this study was to mimic the degenerative IVD microenvironment, and to investigate the effect of a variety of PBM conditions (wavelength: 635, 525, and 470 nm; energy density: 16, 32, and 64 J/cm2) on the production of ECM-modifying-enzymes by AF cells under degenerative conditions induced by macrophage-conditioned medium (MCM), which contains pro-inflammatory cytokines such as TNF-α and IL-β secreted by macrophage during the development of intervertebral disk inflammation. We showed that the MCM-stimulated AF cells express imbalanced ratios of TIMPs (TIMP-1 and TIMP-2) and MMPs (MMP-1 and MMP-3). PBM selectively modulated the production of ECM-modifying enzymes in AF cells. These results suggest that PBM can be a therapeutic tool for degenerative IVD disorders.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalLasers in Medical Science
DOIs
Publication statusAccepted/In press - 2016 Mar 17

Fingerprint

Intervertebral Disc Degeneration
Extracellular Matrix
Macrophages
Enzymes
Tissue Inhibitor of Metalloproteinases
Conditioned Culture Medium
Cytokines
Tumor Necrosis Factor-alpha
Tissue Inhibitor of Metalloproteinase-2
Matrix Metalloproteinase 3
Matrix Metalloproteinase 1
Tissue Inhibitor of Metalloproteinase-1
Matrix Metalloproteinase Inhibitors
Intervertebral Disc
Low Back Pain
Matrix Metalloproteinases
Interleukin-1beta
Wound Healing
Spine
Neutrophils

Keywords

  • Extracellular matrix
  • Human annulus fibrosus
  • Inflammation
  • Intervertebral disk degeneration
  • Photobiomodulation

ASJC Scopus subject areas

  • Surgery
  • Dermatology

Cite this

Photobiomodulation on human annulus fibrosus cells during the intervertebral disk degeneration : extracellular matrix-modifying enzymes. / Hwang, Min Ho; Kim, Kyoung Soo; Yoo, Chang Min; Shin, Jae Hee; Nam, Hyo Geun; Jeong, Jin Su; Kim, Joo-Han; Lee, Kwang Ho; Choi, Hyuk.

In: Lasers in Medical Science, 17.03.2016, p. 1-11.

Research output: Contribution to journalArticle

Hwang, Min Ho ; Kim, Kyoung Soo ; Yoo, Chang Min ; Shin, Jae Hee ; Nam, Hyo Geun ; Jeong, Jin Su ; Kim, Joo-Han ; Lee, Kwang Ho ; Choi, Hyuk. / Photobiomodulation on human annulus fibrosus cells during the intervertebral disk degeneration : extracellular matrix-modifying enzymes. In: Lasers in Medical Science. 2016 ; pp. 1-11.
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abstract = "Destruction of extracellular matrix (ECM) leads to degeneration of the intervertebral disk (IVD), which is a major contributor to many spine disorders. IVD degeneration is induced by pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β), which are secreted by immune cells, including macrophages and neutrophils. The cytokines modulate ECM-modifying enzymes such as matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in human annulus fibrosus (AF) cells. The resulting imbalance in catabolic and anabolic enzymes can cause generalized back, neck, and low back pain (LBP). Photobiomodulation (PBM) is known to regulate inflammatory responses and wound healing. The aim of this study was to mimic the degenerative IVD microenvironment, and to investigate the effect of a variety of PBM conditions (wavelength: 635, 525, and 470 nm; energy density: 16, 32, and 64 J/cm2) on the production of ECM-modifying-enzymes by AF cells under degenerative conditions induced by macrophage-conditioned medium (MCM), which contains pro-inflammatory cytokines such as TNF-α and IL-β secreted by macrophage during the development of intervertebral disk inflammation. We showed that the MCM-stimulated AF cells express imbalanced ratios of TIMPs (TIMP-1 and TIMP-2) and MMPs (MMP-1 and MMP-3). PBM selectively modulated the production of ECM-modifying enzymes in AF cells. These results suggest that PBM can be a therapeutic tool for degenerative IVD disorders.",
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AB - Destruction of extracellular matrix (ECM) leads to degeneration of the intervertebral disk (IVD), which is a major contributor to many spine disorders. IVD degeneration is induced by pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β), which are secreted by immune cells, including macrophages and neutrophils. The cytokines modulate ECM-modifying enzymes such as matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in human annulus fibrosus (AF) cells. The resulting imbalance in catabolic and anabolic enzymes can cause generalized back, neck, and low back pain (LBP). Photobiomodulation (PBM) is known to regulate inflammatory responses and wound healing. The aim of this study was to mimic the degenerative IVD microenvironment, and to investigate the effect of a variety of PBM conditions (wavelength: 635, 525, and 470 nm; energy density: 16, 32, and 64 J/cm2) on the production of ECM-modifying-enzymes by AF cells under degenerative conditions induced by macrophage-conditioned medium (MCM), which contains pro-inflammatory cytokines such as TNF-α and IL-β secreted by macrophage during the development of intervertebral disk inflammation. We showed that the MCM-stimulated AF cells express imbalanced ratios of TIMPs (TIMP-1 and TIMP-2) and MMPs (MMP-1 and MMP-3). PBM selectively modulated the production of ECM-modifying enzymes in AF cells. These results suggest that PBM can be a therapeutic tool for degenerative IVD disorders.

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