Photodissociation tandem mass spectrometry at 266 nm of an aliphatic peptide derivatized with phenyl isothiocyanate and 4-sulfophenyl isothiocyanate

Yeon Oh Joo, Hee Moon Jeong, Ho Lee Yong, Seok Won Hyung, Sang Won Lee, Soo Kim Myung

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Photodissociation (PD) tandem mass spectra have been obtained at 266 nm for the protonated molecules of a tryptic peptide, ASHLGLAR, and of its phenyl isothiocyanate and 4-sulfophenyl isothiocyanate derivatives, generated by matrix-assisted laser desorption/ionization. Derivatization with the aromatic chromophores greatly reduced the intensity of the laser required for efficient PD. Major fragment ions observed in the three spectra are quite similar. General features of the PD tandem mass spectra and their potential utility for analytical purposes are discussed.

Original languageEnglish
Pages (from-to)1283-1288
Number of pages6
JournalRapid Communications in Mass Spectrometry
Volume19
Issue number10
DOIs
Publication statusPublished - 2005

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

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