TY - JOUR
T1 - Photoluminescent Response of Poly(3-methylthiophene)-DNA Single Nanowire Correlating to Nucleotide-Mismatch Locus in DNA–DNA Hybridization
AU - Park, Dong Hyuk
AU - Cui, Chunzhi
AU - Ahn, Dong June
N1 - Funding Information:
D.H.P. and C.C. contributed equally to this work. This work was supported by National Research Foundation (NRF 2017R1A2B3006770 and 2017R1A2B1009314), the Education Department of Jilin Province (JJKH20191121KJ), and the Korea University Grant.
Funding Information:
D.H.P. and C.C. contributed equally to this work. This work was supported by National Research Foundation (NRF 2017R1A2B3006770 and 2017R1A2B1009314), the Education Department of Jilin Province (JJKH20191121KJ), and the Korea University Grant.
Publisher Copyright:
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/11/1
Y1 - 2020/11/1
N2 - π-Conjugated polymers have become qualified candidates for biosensing owing to their unique optoelectronic properties and excellent biocompatibility. In this contribution, nucleotide mismatches in DNA hybridization, being variable in position, are reflected in a stark manner by poly(3-methylthiophene) (P3MT) nanowires (NWs), in which probe DNA sequence is properly functionalized. Selected as the systematic investigation are complementary target DNA (tDNA), random sequence DNA, and three kinds of 1-mer mismatched tDNAs with different mismatch loci away from the NW's surface. Nanoscale optical observation of the single P3MT NWs in solid states reveals that the more distant the mismatch position is from the surface, the higher the photoluminescence (PL) occurs, while the complementary sequence yields the highest but the random one remains the lowest. Hence, the PL intensity increases with the relative length of the DNA–DNA hybridization from the surface. These results deliver a new basis that π-conjugated polymers can be potentially applicable to detailed nucleotide analyses as in single nucleotide polymorphism.
AB - π-Conjugated polymers have become qualified candidates for biosensing owing to their unique optoelectronic properties and excellent biocompatibility. In this contribution, nucleotide mismatches in DNA hybridization, being variable in position, are reflected in a stark manner by poly(3-methylthiophene) (P3MT) nanowires (NWs), in which probe DNA sequence is properly functionalized. Selected as the systematic investigation are complementary target DNA (tDNA), random sequence DNA, and three kinds of 1-mer mismatched tDNAs with different mismatch loci away from the NW's surface. Nanoscale optical observation of the single P3MT NWs in solid states reveals that the more distant the mismatch position is from the surface, the higher the photoluminescence (PL) occurs, while the complementary sequence yields the highest but the random one remains the lowest. Hence, the PL intensity increases with the relative length of the DNA–DNA hybridization from the surface. These results deliver a new basis that π-conjugated polymers can be potentially applicable to detailed nucleotide analyses as in single nucleotide polymorphism.
KW - DNA
KW - photoluminescence
KW - poly(3-methylthiophene)
KW - π-conjugated polymers
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U2 - 10.1002/marc.202000164
DO - 10.1002/marc.202000164
M3 - Article
C2 - 32578310
AN - SCOPUS:85087210652
VL - 41
JO - Macromolecular Rapid Communications
JF - Macromolecular Rapid Communications
SN - 1022-1336
IS - 21
M1 - 2000164
ER -