TY - JOUR
T1 - Photon-directed multiplexed enzymatic DNA synthesis for molecular digital data storage
AU - Lee, Howon
AU - Wiegand, Daniel J.
AU - Griswold, Kettner
AU - Punthambaker, Sukanya
AU - Chun, Honggu
AU - Kohman, Richie E.
AU - Church, George M.
N1 - Funding Information:
The authors would like to thank Dr. Henry Lee for useful discussions regarding digital data storage, enzyme kinetics, and next-generation sequencing. The authors like to additionally thank Mr. Benjamin Vieira for the helpful discussion of musical nomenclature. K.G. is supported under Graduate Fellowships from the Fannie and John Hertz Foundation and the Charles Stark Draper Laboratory. This work was funded by the Wyss Institute for Biologically Inspired Engineering, Boston, Massachusetts.
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - New storage technologies are needed to keep up with the global demands of data generation. DNA is an ideal storage medium due to its stability, information density and ease-of-readout with advanced sequencing techniques. However, progress in writing DNA is stifled by the continued reliance on chemical synthesis methods. The enzymatic synthesis of DNA is a promising alternative, but thus far has not been well demonstrated in a parallelized manner. Here, we report a multiplexed enzymatic DNA synthesis method using maskless photolithography. Rapid uncaging of Co2+ ions by patterned UV light activates Terminal deoxynucleotidyl Transferase (TdT) for spatially-selective synthesis on an array surface. Spontaneous quenching of reactions by the diffusion of excess caging molecules confines synthesis to light patterns and controls the extension length. We show that our multiplexed synthesis method can be used to store digital data by encoding 12 unique DNA oligonucleotide sequences with video game music, which is equivalent to 84 trits or 110 bits of data.
AB - New storage technologies are needed to keep up with the global demands of data generation. DNA is an ideal storage medium due to its stability, information density and ease-of-readout with advanced sequencing techniques. However, progress in writing DNA is stifled by the continued reliance on chemical synthesis methods. The enzymatic synthesis of DNA is a promising alternative, but thus far has not been well demonstrated in a parallelized manner. Here, we report a multiplexed enzymatic DNA synthesis method using maskless photolithography. Rapid uncaging of Co2+ ions by patterned UV light activates Terminal deoxynucleotidyl Transferase (TdT) for spatially-selective synthesis on an array surface. Spontaneous quenching of reactions by the diffusion of excess caging molecules confines synthesis to light patterns and controls the extension length. We show that our multiplexed synthesis method can be used to store digital data by encoding 12 unique DNA oligonucleotide sequences with video game music, which is equivalent to 84 trits or 110 bits of data.
UR - http://www.scopus.com/inward/record.url?scp=85092609646&partnerID=8YFLogxK
U2 - 10.1038/s41467-020-18681-5
DO - 10.1038/s41467-020-18681-5
M3 - Article
C2 - 33067441
AN - SCOPUS:85092609646
VL - 11
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 5246
ER -