Abstract
The etiology of intervertebral disc (IVD) degeneration accompanied by low back pain (LBP) is largely unknown, and there are no curative therapies. Painful IVD degeneration is associated with infiltrated macrophage-mediated inflammatory response of human nucleus pulposus (NP) cells. The present study aimed to address the hypothesis that pro-inflammatory cytokines derived from macrophages lead to the altered molecular phenotype of human NP cells and to investigate the effects of phototherapy (630, 525, 465 nm with 16, 32, 64 J/cm2) on pain-related cytokine interleukin (IL)-6 and chemokine IL-8 under inflammatory conditions in human NP cells. Human NP cells were treated with soluble factors derived from macrophages in an inflammatory microenvironment, similar to that found in degenerative IVD. Human NP cells were also treated with phototherapy (630, 525, 465 nm with 16, 32, 64 J/cm2), and their cytokine and chemokine levels were detected. The soluble factors caused modulated expression of IL-6, IL-8, and matrix metalloproteinases (MMPs) at the gene and protein levels, causing a shift toward matrix catabolism through the expression of MMPs and increased pain-related factors via preferential activation of the nuclear factor-kappa B (NF-κB) p50 protein. Importantly, phototherapy attenuated the protein and gene expression of pain-related factor IL-6 at all doses and wavelengths. Interestingly, phototherapy also modulated the protein and gene expression of IL-8, which is responsible for the anabolic response, at a wavelength of 465 nm at all doses, in human NP cells. These findings suggested that phototherapy, at an optimal dose and wavelength, might be a useful therapeutic tool to treat IVD degeneration.
| Original language | English |
|---|---|
| Pages (from-to) | 1-10 |
| Number of pages | 10 |
| Journal | Lasers in Medical Science |
| DOIs | |
| Publication status | Accepted/In press - 2018 Mar 3 |
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Keywords
- Inflammation
- Intervertebral disc degeneration
- Macrophage
- Nucleus pulposus cell
- Phototherapy
ASJC Scopus subject areas
- Surgery
- Dermatology
Cite this
Phototherapy suppresses inflammation in human nucleus pulposus cells for intervertebral disc degeneration. / Hwang, Min Ho; Son, Hyeong Guk; Lee, Jae Won; Yoo, Chang Min; Shin, Jae Hee; Nam, Hyo Geun; Lim, Hyun Jung; Baek, Seung Min; Park, Jeong Hun; Kim, Joo-Han; Choi, Hyuk.
In: Lasers in Medical Science, 03.03.2018, p. 1-10.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Phototherapy suppresses inflammation in human nucleus pulposus cells for intervertebral disc degeneration
AU - Hwang, Min Ho
AU - Son, Hyeong Guk
AU - Lee, Jae Won
AU - Yoo, Chang Min
AU - Shin, Jae Hee
AU - Nam, Hyo Geun
AU - Lim, Hyun Jung
AU - Baek, Seung Min
AU - Park, Jeong Hun
AU - Kim, Joo-Han
AU - Choi, Hyuk
PY - 2018/3/3
Y1 - 2018/3/3
N2 - The etiology of intervertebral disc (IVD) degeneration accompanied by low back pain (LBP) is largely unknown, and there are no curative therapies. Painful IVD degeneration is associated with infiltrated macrophage-mediated inflammatory response of human nucleus pulposus (NP) cells. The present study aimed to address the hypothesis that pro-inflammatory cytokines derived from macrophages lead to the altered molecular phenotype of human NP cells and to investigate the effects of phototherapy (630, 525, 465 nm with 16, 32, 64 J/cm2) on pain-related cytokine interleukin (IL)-6 and chemokine IL-8 under inflammatory conditions in human NP cells. Human NP cells were treated with soluble factors derived from macrophages in an inflammatory microenvironment, similar to that found in degenerative IVD. Human NP cells were also treated with phototherapy (630, 525, 465 nm with 16, 32, 64 J/cm2), and their cytokine and chemokine levels were detected. The soluble factors caused modulated expression of IL-6, IL-8, and matrix metalloproteinases (MMPs) at the gene and protein levels, causing a shift toward matrix catabolism through the expression of MMPs and increased pain-related factors via preferential activation of the nuclear factor-kappa B (NF-κB) p50 protein. Importantly, phototherapy attenuated the protein and gene expression of pain-related factor IL-6 at all doses and wavelengths. Interestingly, phototherapy also modulated the protein and gene expression of IL-8, which is responsible for the anabolic response, at a wavelength of 465 nm at all doses, in human NP cells. These findings suggested that phototherapy, at an optimal dose and wavelength, might be a useful therapeutic tool to treat IVD degeneration.
AB - The etiology of intervertebral disc (IVD) degeneration accompanied by low back pain (LBP) is largely unknown, and there are no curative therapies. Painful IVD degeneration is associated with infiltrated macrophage-mediated inflammatory response of human nucleus pulposus (NP) cells. The present study aimed to address the hypothesis that pro-inflammatory cytokines derived from macrophages lead to the altered molecular phenotype of human NP cells and to investigate the effects of phototherapy (630, 525, 465 nm with 16, 32, 64 J/cm2) on pain-related cytokine interleukin (IL)-6 and chemokine IL-8 under inflammatory conditions in human NP cells. Human NP cells were treated with soluble factors derived from macrophages in an inflammatory microenvironment, similar to that found in degenerative IVD. Human NP cells were also treated with phototherapy (630, 525, 465 nm with 16, 32, 64 J/cm2), and their cytokine and chemokine levels were detected. The soluble factors caused modulated expression of IL-6, IL-8, and matrix metalloproteinases (MMPs) at the gene and protein levels, causing a shift toward matrix catabolism through the expression of MMPs and increased pain-related factors via preferential activation of the nuclear factor-kappa B (NF-κB) p50 protein. Importantly, phototherapy attenuated the protein and gene expression of pain-related factor IL-6 at all doses and wavelengths. Interestingly, phototherapy also modulated the protein and gene expression of IL-8, which is responsible for the anabolic response, at a wavelength of 465 nm at all doses, in human NP cells. These findings suggested that phototherapy, at an optimal dose and wavelength, might be a useful therapeutic tool to treat IVD degeneration.
KW - Inflammation
KW - Intervertebral disc degeneration
KW - Macrophage
KW - Nucleus pulposus cell
KW - Phototherapy
UR - http://www.scopus.com/inward/record.url?scp=85045041123&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85045041123&partnerID=8YFLogxK
U2 - 10.1007/s10103-018-2470-4
DO - 10.1007/s10103-018-2470-4
M3 - Article
C2 - 29502159
AN - SCOPUS:85045041123
SP - 1
EP - 10
JO - Lasers in Medical Science
JF - Lasers in Medical Science
SN - 0268-8921
ER -