Phylogenetic analyses of the isonychiid mayflies (Ephemeroptera: Isonychiidae) in the northeast palearctic region

Rie Saito, Jaeick Jo, Kazuki Sekiné, Yeon Jae Bae, Koji Tojo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We investigated the molecular phylogeny of isonychiid mayflies inhabiting the East Palearctic region, Isonychia (Isonychia) japonica, Isonychia (Isonychia) ignota, Isonychia (Isonychia) ussurica and Isonychia (Prinoides) shima. We discuss their genetic structures, phylogeny and phylogeography. We collected a total of 100 specimens of isonychiid mayfly species from 47 localities of the Northeast Palearctic region (the Japanese archipelago, the Korean peninsula, the Russian Far East and Mongolia). We analyzed the DNA sequences at the mtDNA COI and 16S rRNA regions, and the nuDNA Histone H3 region. As a result of our genetic analyses of the four Northeast Palearctic isonychiid mayflies, their monophyly at the species level was supported by both the mtDNA (COI and 16S rRNA regions) and the nuDNA (Histone H3 region). In addition, it also became clear that significantly large genetic differentiation exists at the inter-species level; thus, the relationship of "shima+(japonica+(ignota+ussurica))" is supported. Among the four isonychiid mayflies of the Northeast Palearctic area, I. (P.) shima was shown to be a basal-most linage within the included species.

Original languageEnglish
JournalEntomological Research
DOIs
Publication statusAccepted/In press - 2016

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Ephemeroptera
phylogeny
histones
mitochondrial DNA
ribosomal RNA
Mongolia
phylogeography
East Asia
monophyly
Korean Peninsula
nucleotide sequences
genetic variation

Keywords

  • DNA barcoding
  • East Asia
  • Genetic structure
  • Isonychia
  • Molecular phylogeny

ASJC Scopus subject areas

  • Insect Science

Cite this

Phylogenetic analyses of the isonychiid mayflies (Ephemeroptera : Isonychiidae) in the northeast palearctic region. / Saito, Rie; Jo, Jaeick; Sekiné, Kazuki; Bae, Yeon Jae; Tojo, Koji.

In: Entomological Research, 2016.

Research output: Contribution to journalArticle

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AU - Bae, Yeon Jae

AU - Tojo, Koji

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AB - We investigated the molecular phylogeny of isonychiid mayflies inhabiting the East Palearctic region, Isonychia (Isonychia) japonica, Isonychia (Isonychia) ignota, Isonychia (Isonychia) ussurica and Isonychia (Prinoides) shima. We discuss their genetic structures, phylogeny and phylogeography. We collected a total of 100 specimens of isonychiid mayfly species from 47 localities of the Northeast Palearctic region (the Japanese archipelago, the Korean peninsula, the Russian Far East and Mongolia). We analyzed the DNA sequences at the mtDNA COI and 16S rRNA regions, and the nuDNA Histone H3 region. As a result of our genetic analyses of the four Northeast Palearctic isonychiid mayflies, their monophyly at the species level was supported by both the mtDNA (COI and 16S rRNA regions) and the nuDNA (Histone H3 region). In addition, it also became clear that significantly large genetic differentiation exists at the inter-species level; thus, the relationship of "shima+(japonica+(ignota+ussurica))" is supported. Among the four isonychiid mayflies of the Northeast Palearctic area, I. (P.) shima was shown to be a basal-most linage within the included species.

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