Phylogenetic relationships of the HA and NA genes between vaccine and seasonal influenza A(H3N2) strains in Korea

Jin Il Kim, Ilseob Lee, Sehee Park, Joon Yong Bae, Kirim Yoo, Hee-Jin Cheong, Ji Yun Noh, Kyung Wook Hong, Philippe Lemey, Bram Vrancken, Juwon Kim, Misun Nam, Soo Hyeon Yun, Woo In Cho, Joon-Young Song, Woo Joo Kim, Mee Sook Park, Jin Won Song, Sun-Ho Kee, Ki-Joon SongMan-Seong Park

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Seasonal influenza is caused by two influenza A subtype (H1N1 and H3N2) and two influenza B lineage (Victoria and Yamagata) viruses. Of these antigenically distinct viruses, the H3N2 virus was consistently detected in substantial proportions in Korea during the 2010/ 11-2013/14 seasons when compared to the other viruses and appeared responsible for the influenza-like illness rate peak during the first half of the 2011/12 season. To further scrutinize possible causes for this, we investigated the evolutionary and serological relationships between the vaccine and Korean H3N2 strains during the 2011/12 season for the main antigenic determinants of influenza viruses, the hemagglutinin (HA) and neuraminidase (NA) genes. In the 2011/12 season, when the number of H3N2 cases peaked, the majority of the Korean strains did not belong to the HA clade of A/Perth/16/2009 vaccine, and no Korean strains were of this lineage in the NA segment. In a serological assay, post-vaccinated human sera exhibited much reduced hemagglutination inhibition antibody titers against the non-vaccine clade Korean H3N2 strains. Moreover, Korean strains harbored several amino acid differences in the HA antigenic sites and in the NA with respect to vaccine lineages during this season. Of these, the HA antigenic site C residues 45 and 261 and the NA residue 81 appeared to be the signatures of positive selection. In subsequent seasons, when H3N2 cases were lower, the HA and NA genes of vaccine and Korean strains were more phylogenetically related to each other. Combined, our results provide indirect support for using phylogenetic clustering patterns of the HA and possibly also the NA genes in the selection of vaccine viruses and the assessment of vaccine effectiveness.

Original languageEnglish
Article numbere0172059
JournalPLoS One
Volume12
Issue number3
DOIs
Publication statusPublished - 2017 Mar 1

Fingerprint

sialidase
Influenza Vaccines
Hemagglutinins
Neuraminidase
hemagglutinins
Korea
Viruses
influenza
Korean Peninsula
Vaccines
Genes
vaccines
Human Influenza
phylogeny
viruses
genes
H3N2 Subtype Influenza A Virus
Victoria
Hemagglutination
Orthomyxoviridae

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Phylogenetic relationships of the HA and NA genes between vaccine and seasonal influenza A(H3N2) strains in Korea. / Kim, Jin Il; Lee, Ilseob; Park, Sehee; Bae, Joon Yong; Yoo, Kirim; Cheong, Hee-Jin; Noh, Ji Yun; Hong, Kyung Wook; Lemey, Philippe; Vrancken, Bram; Kim, Juwon; Nam, Misun; Yun, Soo Hyeon; Cho, Woo In; Song, Joon-Young; Kim, Woo Joo; Park, Mee Sook; Song, Jin Won; Kee, Sun-Ho; Song, Ki-Joon; Park, Man-Seong.

In: PLoS One, Vol. 12, No. 3, e0172059, 01.03.2017.

Research output: Contribution to journalArticle

Kim, Jin Il ; Lee, Ilseob ; Park, Sehee ; Bae, Joon Yong ; Yoo, Kirim ; Cheong, Hee-Jin ; Noh, Ji Yun ; Hong, Kyung Wook ; Lemey, Philippe ; Vrancken, Bram ; Kim, Juwon ; Nam, Misun ; Yun, Soo Hyeon ; Cho, Woo In ; Song, Joon-Young ; Kim, Woo Joo ; Park, Mee Sook ; Song, Jin Won ; Kee, Sun-Ho ; Song, Ki-Joon ; Park, Man-Seong. / Phylogenetic relationships of the HA and NA genes between vaccine and seasonal influenza A(H3N2) strains in Korea. In: PLoS One. 2017 ; Vol. 12, No. 3.
@article{b53d0c6de6624399b2d916c2f4982e0f,
title = "Phylogenetic relationships of the HA and NA genes between vaccine and seasonal influenza A(H3N2) strains in Korea",
abstract = "Seasonal influenza is caused by two influenza A subtype (H1N1 and H3N2) and two influenza B lineage (Victoria and Yamagata) viruses. Of these antigenically distinct viruses, the H3N2 virus was consistently detected in substantial proportions in Korea during the 2010/ 11-2013/14 seasons when compared to the other viruses and appeared responsible for the influenza-like illness rate peak during the first half of the 2011/12 season. To further scrutinize possible causes for this, we investigated the evolutionary and serological relationships between the vaccine and Korean H3N2 strains during the 2011/12 season for the main antigenic determinants of influenza viruses, the hemagglutinin (HA) and neuraminidase (NA) genes. In the 2011/12 season, when the number of H3N2 cases peaked, the majority of the Korean strains did not belong to the HA clade of A/Perth/16/2009 vaccine, and no Korean strains were of this lineage in the NA segment. In a serological assay, post-vaccinated human sera exhibited much reduced hemagglutination inhibition antibody titers against the non-vaccine clade Korean H3N2 strains. Moreover, Korean strains harbored several amino acid differences in the HA antigenic sites and in the NA with respect to vaccine lineages during this season. Of these, the HA antigenic site C residues 45 and 261 and the NA residue 81 appeared to be the signatures of positive selection. In subsequent seasons, when H3N2 cases were lower, the HA and NA genes of vaccine and Korean strains were more phylogenetically related to each other. Combined, our results provide indirect support for using phylogenetic clustering patterns of the HA and possibly also the NA genes in the selection of vaccine viruses and the assessment of vaccine effectiveness.",
author = "Kim, {Jin Il} and Ilseob Lee and Sehee Park and Bae, {Joon Yong} and Kirim Yoo and Hee-Jin Cheong and Noh, {Ji Yun} and Hong, {Kyung Wook} and Philippe Lemey and Bram Vrancken and Juwon Kim and Misun Nam and Yun, {Soo Hyeon} and Cho, {Woo In} and Joon-Young Song and Kim, {Woo Joo} and Park, {Mee Sook} and Song, {Jin Won} and Sun-Ho Kee and Ki-Joon Song and Man-Seong Park",
year = "2017",
month = "3",
day = "1",
doi = "10.1371/journal.pone.0172059",
language = "English",
volume = "12",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",

}

TY - JOUR

T1 - Phylogenetic relationships of the HA and NA genes between vaccine and seasonal influenza A(H3N2) strains in Korea

AU - Kim, Jin Il

AU - Lee, Ilseob

AU - Park, Sehee

AU - Bae, Joon Yong

AU - Yoo, Kirim

AU - Cheong, Hee-Jin

AU - Noh, Ji Yun

AU - Hong, Kyung Wook

AU - Lemey, Philippe

AU - Vrancken, Bram

AU - Kim, Juwon

AU - Nam, Misun

AU - Yun, Soo Hyeon

AU - Cho, Woo In

AU - Song, Joon-Young

AU - Kim, Woo Joo

AU - Park, Mee Sook

AU - Song, Jin Won

AU - Kee, Sun-Ho

AU - Song, Ki-Joon

AU - Park, Man-Seong

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Seasonal influenza is caused by two influenza A subtype (H1N1 and H3N2) and two influenza B lineage (Victoria and Yamagata) viruses. Of these antigenically distinct viruses, the H3N2 virus was consistently detected in substantial proportions in Korea during the 2010/ 11-2013/14 seasons when compared to the other viruses and appeared responsible for the influenza-like illness rate peak during the first half of the 2011/12 season. To further scrutinize possible causes for this, we investigated the evolutionary and serological relationships between the vaccine and Korean H3N2 strains during the 2011/12 season for the main antigenic determinants of influenza viruses, the hemagglutinin (HA) and neuraminidase (NA) genes. In the 2011/12 season, when the number of H3N2 cases peaked, the majority of the Korean strains did not belong to the HA clade of A/Perth/16/2009 vaccine, and no Korean strains were of this lineage in the NA segment. In a serological assay, post-vaccinated human sera exhibited much reduced hemagglutination inhibition antibody titers against the non-vaccine clade Korean H3N2 strains. Moreover, Korean strains harbored several amino acid differences in the HA antigenic sites and in the NA with respect to vaccine lineages during this season. Of these, the HA antigenic site C residues 45 and 261 and the NA residue 81 appeared to be the signatures of positive selection. In subsequent seasons, when H3N2 cases were lower, the HA and NA genes of vaccine and Korean strains were more phylogenetically related to each other. Combined, our results provide indirect support for using phylogenetic clustering patterns of the HA and possibly also the NA genes in the selection of vaccine viruses and the assessment of vaccine effectiveness.

AB - Seasonal influenza is caused by two influenza A subtype (H1N1 and H3N2) and two influenza B lineage (Victoria and Yamagata) viruses. Of these antigenically distinct viruses, the H3N2 virus was consistently detected in substantial proportions in Korea during the 2010/ 11-2013/14 seasons when compared to the other viruses and appeared responsible for the influenza-like illness rate peak during the first half of the 2011/12 season. To further scrutinize possible causes for this, we investigated the evolutionary and serological relationships between the vaccine and Korean H3N2 strains during the 2011/12 season for the main antigenic determinants of influenza viruses, the hemagglutinin (HA) and neuraminidase (NA) genes. In the 2011/12 season, when the number of H3N2 cases peaked, the majority of the Korean strains did not belong to the HA clade of A/Perth/16/2009 vaccine, and no Korean strains were of this lineage in the NA segment. In a serological assay, post-vaccinated human sera exhibited much reduced hemagglutination inhibition antibody titers against the non-vaccine clade Korean H3N2 strains. Moreover, Korean strains harbored several amino acid differences in the HA antigenic sites and in the NA with respect to vaccine lineages during this season. Of these, the HA antigenic site C residues 45 and 261 and the NA residue 81 appeared to be the signatures of positive selection. In subsequent seasons, when H3N2 cases were lower, the HA and NA genes of vaccine and Korean strains were more phylogenetically related to each other. Combined, our results provide indirect support for using phylogenetic clustering patterns of the HA and possibly also the NA genes in the selection of vaccine viruses and the assessment of vaccine effectiveness.

UR - http://www.scopus.com/inward/record.url?scp=85014501389&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85014501389&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0172059

DO - 10.1371/journal.pone.0172059

M3 - Article

C2 - 28257427

AN - SCOPUS:85014501389

VL - 12

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 3

M1 - e0172059

ER -