Physiological and metabolic responses for hexadecane degradation in Acinetobacter oleivorans DR1

Jaejoon Jung, Jaemin Noh, Woojun Park

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The hexadecane degradation of Acinetobacter oleivorans DR1 was evaluated with changes in temperature and ionic salt contents. Hexadecane degradation of strain DR1 was reduced markedly by the presence of sodium chloride (but not potassium chloride). High temperature (37°C) was also shown to inhibit the motility, biofilm formation, and hexadecane biodégradation. The biofilm formation of strain DR1 on the oil-water interface might prove to be a critical physiological feature for the degradation of hexadecane. The positive relationship between biofilm formation and hexadecane degradation could be observed at 30° C, but not at low temperatures (25°C). Alterations in cell hydrophobicity and EPS production by temperature and salts were not correlated with biofilm formation and hexadecane degradation. Our proteomic analyses have demonstrated that metabolic changes through the glyoxylate pathway are important for efficient degradation of hexadecane. Proteins involved in fatty acid metabolism, gluconeogenesis, and oxidative stress defense proteins appear to be highly expressed during biodégradation of hexadecane. These results suggested that biofilm formation and oxidative stress defense are important physiological responses for hexadecane degradation along with metabolic switch to glyoxylate pathway in strain DR1.

Original languageEnglish
Pages (from-to)208-215
Number of pages8
JournalJournal of Microbiology
Volume49
Issue number2
DOIs
Publication statusPublished - 2011 Apr 1

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Acinetobacter
Biofilms
Temperature
Oxidative Stress
Salts
n-hexadecane
Gluconeogenesis
Potassium Chloride
Heat-Shock Proteins
Hydrophobic and Hydrophilic Interactions
Sodium Chloride
Proteomics
Oils
Fatty Acids
Water

Keywords

  • Acinetobacter
  • biofilm
  • hydrocarbon degradation
  • motility
  • proteomic analysis

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Physiological and metabolic responses for hexadecane degradation in Acinetobacter oleivorans DR1. / Jung, Jaejoon; Noh, Jaemin; Park, Woojun.

In: Journal of Microbiology, Vol. 49, No. 2, 01.04.2011, p. 208-215.

Research output: Contribution to journalArticle

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