Abstract
Yeast with multiple tolerance onto harsh conditions has a number of advantages for bioethanol production. In this study, an alcohol yeast of Issatchenkia orientalis MTY1 is isolated in a Korean winery and its multiple tolerance against high temperature and acidic conditions is characterized in microaerobic batch cultures and by metabolomic analysis. In a series of batch cultures using 100gL-1 glucose, I. orientalis MTY1 possesses wider growth ranges at pH 2-8 and 30-45°C than a conventional yeast of Saccharomyces cerevisiae D452-2. Moreover, I. orientalis MTY1 showes higher cell growth and ethanol productivity in the presence of acetic acid or furfural than S. cerevisiae D452-2. I. orientalis MTY1 produces 41.4gL-1 ethanol with 1.5gL-1h-1 productivity at 42°C and pH 4.2 in the presence of 4gL-1 acetic acid, whereas a thermo-tolerant yeast of Kluyvermyces marxianus ATCC36907 does not grow. By metabolomics by GC-TOF MS and statistical analysis of 125 metabolite peaks, it is revealed that the thermo-tolerance of I. orientalis MTY1 might be ascribed to higher contents of unsaturated fatty acids, purines and pyrimidines than S. cerevisiae D452-2. Conclusively, I. orientalis MTY1 could be a potent workhorse with multiple tolerance against harsh conditions considered in cellulosic bioethanol production.
| Original language | English |
|---|---|
| Journal | Biotechnology Journal |
| DOIs | |
| Publication status | Accepted/In press - 2017 |
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Keywords
- Bioethanol
- Fermentation
- Issatchenkia orientalis
- Metabolomics
- Multiple tolerance
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Molecular Medicine
Cite this
Physiological and Metabolomic Analysis of Issatchenkia orientalis MTY1 With Multiple Tolerance for Cellulosic Bioethanol Production. / Seong, Yeong Je; Lee, Hye Jin; Lee, Jung Eun; Kim, Sooah; Lee, Do Yup; Kim, Kyoung Heon; Park, Yong Cheol.
In: Biotechnology Journal, 2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Physiological and Metabolomic Analysis of Issatchenkia orientalis MTY1 With Multiple Tolerance for Cellulosic Bioethanol Production
AU - Seong, Yeong Je
AU - Lee, Hye Jin
AU - Lee, Jung Eun
AU - Kim, Sooah
AU - Lee, Do Yup
AU - Kim, Kyoung Heon
AU - Park, Yong Cheol
PY - 2017
Y1 - 2017
N2 - Yeast with multiple tolerance onto harsh conditions has a number of advantages for bioethanol production. In this study, an alcohol yeast of Issatchenkia orientalis MTY1 is isolated in a Korean winery and its multiple tolerance against high temperature and acidic conditions is characterized in microaerobic batch cultures and by metabolomic analysis. In a series of batch cultures using 100gL-1 glucose, I. orientalis MTY1 possesses wider growth ranges at pH 2-8 and 30-45°C than a conventional yeast of Saccharomyces cerevisiae D452-2. Moreover, I. orientalis MTY1 showes higher cell growth and ethanol productivity in the presence of acetic acid or furfural than S. cerevisiae D452-2. I. orientalis MTY1 produces 41.4gL-1 ethanol with 1.5gL-1h-1 productivity at 42°C and pH 4.2 in the presence of 4gL-1 acetic acid, whereas a thermo-tolerant yeast of Kluyvermyces marxianus ATCC36907 does not grow. By metabolomics by GC-TOF MS and statistical analysis of 125 metabolite peaks, it is revealed that the thermo-tolerance of I. orientalis MTY1 might be ascribed to higher contents of unsaturated fatty acids, purines and pyrimidines than S. cerevisiae D452-2. Conclusively, I. orientalis MTY1 could be a potent workhorse with multiple tolerance against harsh conditions considered in cellulosic bioethanol production.
AB - Yeast with multiple tolerance onto harsh conditions has a number of advantages for bioethanol production. In this study, an alcohol yeast of Issatchenkia orientalis MTY1 is isolated in a Korean winery and its multiple tolerance against high temperature and acidic conditions is characterized in microaerobic batch cultures and by metabolomic analysis. In a series of batch cultures using 100gL-1 glucose, I. orientalis MTY1 possesses wider growth ranges at pH 2-8 and 30-45°C than a conventional yeast of Saccharomyces cerevisiae D452-2. Moreover, I. orientalis MTY1 showes higher cell growth and ethanol productivity in the presence of acetic acid or furfural than S. cerevisiae D452-2. I. orientalis MTY1 produces 41.4gL-1 ethanol with 1.5gL-1h-1 productivity at 42°C and pH 4.2 in the presence of 4gL-1 acetic acid, whereas a thermo-tolerant yeast of Kluyvermyces marxianus ATCC36907 does not grow. By metabolomics by GC-TOF MS and statistical analysis of 125 metabolite peaks, it is revealed that the thermo-tolerance of I. orientalis MTY1 might be ascribed to higher contents of unsaturated fatty acids, purines and pyrimidines than S. cerevisiae D452-2. Conclusively, I. orientalis MTY1 could be a potent workhorse with multiple tolerance against harsh conditions considered in cellulosic bioethanol production.
KW - Bioethanol
KW - Fermentation
KW - Issatchenkia orientalis
KW - Metabolomics
KW - Multiple tolerance
UR - http://www.scopus.com/inward/record.url?scp=85029430288&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85029430288&partnerID=8YFLogxK
U2 - 10.1002/biot.201700110
DO - 10.1002/biot.201700110
M3 - Article
C2 - 28843023
AN - SCOPUS:85029430288
JO - Biotechnology Journal
JF - Biotechnology Journal
SN - 1860-6768
ER -