Abstract
Yeast with multiple tolerance onto harsh conditions has a number of advantages for bioethanol production. In this study, an alcohol yeast of Issatchenkia orientalis MTY1 is isolated in a Korean winery and its multiple tolerance against high temperature and acidic conditions is characterized in microaerobic batch cultures and by metabolomic analysis. In a series of batch cultures using 100 g L−1 glucose, I. orientalis MTY1 possesses wider growth ranges at pH 2–8 and 30–45 °C than a conventional yeast of Saccharomyces cerevisiae D452-2. Moreover, I. orientalis MTY1 showes higher cell growth and ethanol productivity in the presence of acetic acid or furfural than S. cerevisiae D452-2. I. orientalis MTY1 produces 41.4 g L−1 ethanol with 1.5 g L−1 h−1 productivity at 42 °C and pH 4.2 in the presence of 4 g L−1 acetic acid, whereas a thermo-tolerant yeast of Kluyvermyces marxianus ATCC36907 does not grow. By metabolomics by GC-TOF MS and statistical analysis of 125 metabolite peaks, it is revealed that the thermo-tolerance of I. orientalis MTY1 might be ascribed to higher contents of unsaturated fatty acids, purines and pyrimidines than S. cerevisiae D452-2. Conclusively, I. orientalis MTY1 could be a potent workhorse with multiple tolerance against harsh conditions considered in cellulosic bioethanol production.
Original language | English |
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Article number | 1700110 |
Journal | Biotechnology Journal |
Volume | 12 |
Issue number | 11 |
DOIs | |
Publication status | Published - 2017 Nov |
Keywords
- Issatchenkia orientalis
- bioethanol
- fermentation
- metabolomics
- multiple tolerance
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Molecular Medicine