Physiological changes and anti-oxidative responses of Arabidopsis plants after acute and chronic γ-irradiation

Eun Jeong Goh, Jin Baek Kim, Wook Jin Kim, Bo Keun Ha, Sang Hoon Kim, Si–Yong –Y Kang, Yong Weon Seo, Dong Sub Kim

Research output: Contribution to journalArticle

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Abstract

To identify the effects of acute and chronic γ-irradiation in Arabidopsis plants, physiological responses and antioxidant-related gene expression were investigated. Seedlings were exposed to 200 Gy of γ-irradiation in acute manner for 1 or 24 h (A1 and A24) or in chronic manner for 1, 2, or 3 weeks (C1 W, C2 W, and C3 W). Plant height, silique number, and silique length in A1 and A24 irradiated plants were significantly reduced when compared to non-irradiated plants. Silique number decreased in response to both acute and chronic irradiation, except with the C3 W treatment, and the number of trichomes dramatically increased in A1 and C1 W. Electron spin resonance signal intensities increased in A1 and in all chronically irradiated plants, but decreased in the A24-treated plant. To investigate the effects of acute and chronic γ-irradiation on antioxidant enzymes, we examined activity of four antioxidant enzymes: catalase (CAT), peroxidase (POD), ascorbate peroxidase, and superoxide dismutase. In general, POD and CAT activities decreased in response to acute and chronic γ-irradiation. Oligonucleotide microarrays were used to investigate transcriptional changes after irradiation. Several genes related to reactive oxygen species signaling were up-regulated after acute and chronic exposure, including genes encoding heat shock factors, zinc finger proteins, NADPH oxidase, WRKY DNA-binding proteins, and calcium binding proteins. Taken together, our data indicate that the responses and activation of antioxidant systems prompted by irradiation exposure are dependent upon the γ-ray dose rate.

Original languageEnglish
Pages (from-to)677-693
Number of pages17
JournalRadiation and Environmental Biophysics
Volume53
Issue number4
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Arabidopsis
irradiation
antioxidants
antioxidant
Antioxidants
catalase
Catalase
Peroxidase
proteins
genes
protein
enzymes
Ascorbate Peroxidases
Trichomes
physiological responses
Calcium-Binding Proteins
enzyme
NADPH Oxidase
Zinc Fingers
Electron Spin Resonance Spectroscopy

ASJC Scopus subject areas

  • Biophysics
  • Environmental Science(all)
  • Radiation

Cite this

Physiological changes and anti-oxidative responses of Arabidopsis plants after acute and chronic γ-irradiation. / Goh, Eun Jeong; Kim, Jin Baek; Kim, Wook Jin; Ha, Bo Keun; Kim, Sang Hoon; Kang, Si–Yong –Y; Seo, Yong Weon; Kim, Dong Sub.

In: Radiation and Environmental Biophysics, Vol. 53, No. 4, 01.01.2014, p. 677-693.

Research output: Contribution to journalArticle

Goh, Eun Jeong ; Kim, Jin Baek ; Kim, Wook Jin ; Ha, Bo Keun ; Kim, Sang Hoon ; Kang, Si–Yong –Y ; Seo, Yong Weon ; Kim, Dong Sub. / Physiological changes and anti-oxidative responses of Arabidopsis plants after acute and chronic γ-irradiation. In: Radiation and Environmental Biophysics. 2014 ; Vol. 53, No. 4. pp. 677-693.
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