Abstract
Obesity is strongly associated with several metabolic and chronic diseases and has become a major public health problem of worldwide concern. This study aimed to investigate the physiological characteristics and anti-obesity effects of Lactobacillus plantarum Q180. Lactobacillus plantarum Q180 was isolated from the faces of healthy adults and found to have a lipase inhibitory activity of 83.61±2.32% and inhibited adipocyte differentiation of 3T3-L1 cells (14.63±1.37%) at a concentration of 100 μg/mL. The strain was investigated for its physiological characteristics. The optimum growth temperature of L. plantarum Q180 was 37°C. Lactobacillus plantarum Q180 showed higher sensitivity to novobiocin in a comparison of fifteen different antibiotics and showed the highest resistance to rifampicin, polymyxin B and vancomycin. The strain showed higher β-galactosidase and N-acetyl-β-glucosaminidase activities. It also did not produce carcinogenic enzymes such as β-glucuronidase. The survival rate of L. plantarum Q180 in MRS broth containing 0.3% bile was 97.8%. Moreover, the strain showed a 97.2% survival rate after incubation for 3 h in pH 2.0. Lactobacillus plantarum Q180 was displayed resistance to Escherichia coli, Salmonella Typhimurium and Staphylococcus aureus with rates of 55.6%, 38.0% and 47.6%, respectively. These results demonstrate that L. plantarum Q180 has potential as a probiotic with anti-obesity effects.
| Original language | English |
|---|---|
| Pages (from-to) | 647-655 |
| Number of pages | 9 |
| Journal | Korean Journal for Food Science of Animal Resources |
| Volume | 34 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2014 Oct 1 |
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Keywords
- Anti-adipogenic activity
- Anti-lipase activity
- Lactobacillus plantarum
- Physiological characteristics
ASJC Scopus subject areas
- Food Science
- Animal Science and Zoology
Cite this
Physiological characteristics and anti-obesity effect of lactobacillus plantarum Q180 isolated from feces. / Park, Sun Young; Cho, Seong A.; Kim, Sae Hun; Lim, Sang Dong.
In: Korean Journal for Food Science of Animal Resources, Vol. 34, No. 5, 01.10.2014, p. 647-655.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Physiological characteristics and anti-obesity effect of lactobacillus plantarum Q180 isolated from feces
AU - Park, Sun Young
AU - Cho, Seong A.
AU - Kim, Sae Hun
AU - Lim, Sang Dong
PY - 2014/10/1
Y1 - 2014/10/1
N2 - Obesity is strongly associated with several metabolic and chronic diseases and has become a major public health problem of worldwide concern. This study aimed to investigate the physiological characteristics and anti-obesity effects of Lactobacillus plantarum Q180. Lactobacillus plantarum Q180 was isolated from the faces of healthy adults and found to have a lipase inhibitory activity of 83.61±2.32% and inhibited adipocyte differentiation of 3T3-L1 cells (14.63±1.37%) at a concentration of 100 μg/mL. The strain was investigated for its physiological characteristics. The optimum growth temperature of L. plantarum Q180 was 37°C. Lactobacillus plantarum Q180 showed higher sensitivity to novobiocin in a comparison of fifteen different antibiotics and showed the highest resistance to rifampicin, polymyxin B and vancomycin. The strain showed higher β-galactosidase and N-acetyl-β-glucosaminidase activities. It also did not produce carcinogenic enzymes such as β-glucuronidase. The survival rate of L. plantarum Q180 in MRS broth containing 0.3% bile was 97.8%. Moreover, the strain showed a 97.2% survival rate after incubation for 3 h in pH 2.0. Lactobacillus plantarum Q180 was displayed resistance to Escherichia coli, Salmonella Typhimurium and Staphylococcus aureus with rates of 55.6%, 38.0% and 47.6%, respectively. These results demonstrate that L. plantarum Q180 has potential as a probiotic with anti-obesity effects.
AB - Obesity is strongly associated with several metabolic and chronic diseases and has become a major public health problem of worldwide concern. This study aimed to investigate the physiological characteristics and anti-obesity effects of Lactobacillus plantarum Q180. Lactobacillus plantarum Q180 was isolated from the faces of healthy adults and found to have a lipase inhibitory activity of 83.61±2.32% and inhibited adipocyte differentiation of 3T3-L1 cells (14.63±1.37%) at a concentration of 100 μg/mL. The strain was investigated for its physiological characteristics. The optimum growth temperature of L. plantarum Q180 was 37°C. Lactobacillus plantarum Q180 showed higher sensitivity to novobiocin in a comparison of fifteen different antibiotics and showed the highest resistance to rifampicin, polymyxin B and vancomycin. The strain showed higher β-galactosidase and N-acetyl-β-glucosaminidase activities. It also did not produce carcinogenic enzymes such as β-glucuronidase. The survival rate of L. plantarum Q180 in MRS broth containing 0.3% bile was 97.8%. Moreover, the strain showed a 97.2% survival rate after incubation for 3 h in pH 2.0. Lactobacillus plantarum Q180 was displayed resistance to Escherichia coli, Salmonella Typhimurium and Staphylococcus aureus with rates of 55.6%, 38.0% and 47.6%, respectively. These results demonstrate that L. plantarum Q180 has potential as a probiotic with anti-obesity effects.
KW - Anti-adipogenic activity
KW - Anti-lipase activity
KW - Lactobacillus plantarum
KW - Physiological characteristics
UR - http://www.scopus.com/inward/record.url?scp=84941649281&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84941649281&partnerID=8YFLogxK
U2 - 10.5851/kosfa.2014.34.5.647
DO - 10.5851/kosfa.2014.34.5.647
M3 - Article
AN - SCOPUS:84941649281
VL - 34
SP - 647
EP - 655
JO - Korean Journal for Food Science of Animal Resources
JF - Korean Journal for Food Science of Animal Resources
SN - 1225-8563
IS - 5
ER -