TY - JOUR
T1 - Picolinafen exerts developmental toxicity via the suppression of oxidative stress and angiogenesis in zebrafish embryos
AU - Lee, Jin Young
AU - Park, Sunwoo
AU - Lim, Whasun
AU - Song, Gwonhwa
N1 - Funding Information:
This research was supported by a grant of the National Research Foundation of Korea (NRF) grant funded by the Ministry of Science and ICT (MSIT) in Republic of Korea. [grant number 2018R1C1B6009048 ].
PY - 2021/1
Y1 - 2021/1
N2 - Picolinafen, a phytoene desaturase-inhibiting herbicide, has been used since 2001 to control the growth of broadleaf weeds. Picolinafen has lower solubility and volatility, and shows lower toxicity to non-target insect species than other types of herbicide. Although picolinafen has been detected in lakes near urban environments and induces chronic toxicity in the mammals, birds, and some aquatic organisms, no study has investigated the toxicity or mode of action of picolinafen in zebrafish. In this study, we demonstrated the lethality and acute LC50 value of picolinafen towards zebrafish embryos. Picolinafen hampered the development of embryos by the induction of morphological abnormalities via apoptosis. Additionally, picolinafen suppressed the generation of reactive oxygen species and angiogenesis. Also, the angiogenesis related genes, flt1 and flt4 mRNA expression was decreased in zebrafish embryos. This study provides a mechanistic understanding of the developmental toxicity of picolinafen in vertebrates.
AB - Picolinafen, a phytoene desaturase-inhibiting herbicide, has been used since 2001 to control the growth of broadleaf weeds. Picolinafen has lower solubility and volatility, and shows lower toxicity to non-target insect species than other types of herbicide. Although picolinafen has been detected in lakes near urban environments and induces chronic toxicity in the mammals, birds, and some aquatic organisms, no study has investigated the toxicity or mode of action of picolinafen in zebrafish. In this study, we demonstrated the lethality and acute LC50 value of picolinafen towards zebrafish embryos. Picolinafen hampered the development of embryos by the induction of morphological abnormalities via apoptosis. Additionally, picolinafen suppressed the generation of reactive oxygen species and angiogenesis. Also, the angiogenesis related genes, flt1 and flt4 mRNA expression was decreased in zebrafish embryos. This study provides a mechanistic understanding of the developmental toxicity of picolinafen in vertebrates.
KW - Angiogenesis
KW - Developmental toxicity
KW - Oxidative stress
KW - Picolinafen
KW - Zebrafish
UR - http://www.scopus.com/inward/record.url?scp=85093692633&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85093692633&partnerID=8YFLogxK
U2 - 10.1016/j.pestbp.2020.104734
DO - 10.1016/j.pestbp.2020.104734
M3 - Article
C2 - 33357556
AN - SCOPUS:85093692633
VL - 171
JO - Pesticide Biochemistry and Physiology
JF - Pesticide Biochemistry and Physiology
SN - 0048-3575
M1 - 104734
ER -