PIF4 promotes expression of LNG1 and LNG2 to induce thermomorphogenic growth in arabidopsis

Geonhee Hwang, Jia Ying Zhu, Young K. Lee, Sara Kim, Thom T. Nguyen, Jungmook Kim, Eunkyoo Oh

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Arabidopsis plants adapt to high ambient temperature by a suite of morphological changes including elongation of hypocotyls and petioles and leaf hyponastic growth. These morphological changes are collectively called thermomorphogenesis and are believed to increase leaf cooling capacity by enhancing transpiration efficiency, thereby increasing tolerance to heat stress. The bHLH transcription factor PHYTOCHROME INTERACTING FACTOR4 (PIF4) has been identified as a major regulator of thermomorphogenic growth. Here, we show that PIF4 promotes the expression of two homologous genes LONGIFOLIA1 (LNG1) and LONGIFOLIA2 (LNG2) that have been reported to regulate leaf morphology. ChIP-Seq analyses and ChIP assays showed that PIF4 directly binds to the promoters of both LNG1 and LNG2. The expression of LNG1 and LNG2 is induced by high temperature in wild type plants. However, the high temperature activation of LNG1 and LNG2 is compromised in the pif4 mutant, indicating that PIF4 directly regulates LNG1 and LNG2 expression in response to high ambient temperatures. We further show that the activities of LNGs support thermomorphogenic growth. The expression of auxin biosynthetic and responsive genes is decreased in the lng quadruple mutant, implying that LNGs promote thermomorphogenic growth by activating the auxin pathway. Together, our results demonstrate that LNG1 and LNG2 are directly regulated by PIF4 and are new components for the regulation of thermomorphogenesis.

Original languageEnglish
Article number1320
JournalFrontiers in Plant Science
Volume8
DOIs
Publication statusPublished - 2017 Jul 25
Externally publishedYes

Fingerprint

phytochrome
Arabidopsis
auxins
ambient temperature
leaves
mutants
petioles
growth regulators
hypocotyls
heat stress
transpiration
temperature
genes
cooling
transcription factors
promoter regions
assays

Keywords

  • Arabidopsis
  • ChIP
  • Gene expression
  • High temperature stress
  • LNG
  • PIF4
  • Thermomorphogenesis

ASJC Scopus subject areas

  • Plant Science

Cite this

PIF4 promotes expression of LNG1 and LNG2 to induce thermomorphogenic growth in arabidopsis. / Hwang, Geonhee; Zhu, Jia Ying; Lee, Young K.; Kim, Sara; Nguyen, Thom T.; Kim, Jungmook; Oh, Eunkyoo.

In: Frontiers in Plant Science, Vol. 8, 1320, 25.07.2017.

Research output: Contribution to journalArticle

Hwang, Geonhee ; Zhu, Jia Ying ; Lee, Young K. ; Kim, Sara ; Nguyen, Thom T. ; Kim, Jungmook ; Oh, Eunkyoo. / PIF4 promotes expression of LNG1 and LNG2 to induce thermomorphogenic growth in arabidopsis. In: Frontiers in Plant Science. 2017 ; Vol. 8.
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AB - Arabidopsis plants adapt to high ambient temperature by a suite of morphological changes including elongation of hypocotyls and petioles and leaf hyponastic growth. These morphological changes are collectively called thermomorphogenesis and are believed to increase leaf cooling capacity by enhancing transpiration efficiency, thereby increasing tolerance to heat stress. The bHLH transcription factor PHYTOCHROME INTERACTING FACTOR4 (PIF4) has been identified as a major regulator of thermomorphogenic growth. Here, we show that PIF4 promotes the expression of two homologous genes LONGIFOLIA1 (LNG1) and LONGIFOLIA2 (LNG2) that have been reported to regulate leaf morphology. ChIP-Seq analyses and ChIP assays showed that PIF4 directly binds to the promoters of both LNG1 and LNG2. The expression of LNG1 and LNG2 is induced by high temperature in wild type plants. However, the high temperature activation of LNG1 and LNG2 is compromised in the pif4 mutant, indicating that PIF4 directly regulates LNG1 and LNG2 expression in response to high ambient temperatures. We further show that the activities of LNGs support thermomorphogenic growth. The expression of auxin biosynthetic and responsive genes is decreased in the lng quadruple mutant, implying that LNGs promote thermomorphogenic growth by activating the auxin pathway. Together, our results demonstrate that LNG1 and LNG2 are directly regulated by PIF4 and are new components for the regulation of thermomorphogenesis.

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