Pig tissue factor pathway inhibitor α fusion immunoglobulin inhibits pig tissue factor activity in human plasma moderately more efficiently than the human counterpart

Chang Yong Choi, Yeon Hui Kim, Joonbeom Bae, Suk Jun Lee, Hyun Kyung Kim, Chung Gyu Park, Taehoon Chun

Research output: Contribution to journalArticle

Abstract

Objective: To determine the efficacy of soluble pig tissue factor pathway inhibitor fusion immunoglobulin (TFPI-Ig) in blocking pig to human xenogeneic blood coagulation. Results: To generate pig TFPI-Ig or human TFPI-Ig, expression vector containing cDNA encoding pig TFPIα or human TFPIα combined with human constant Ig heavy chain region was cloned and introduced into CHO cells. After purification of pig TFPI-Ig and human TFPI-Ig, the inhibition of each recombinant protein on pig tissue factor (TF)-mediated blood coagulation was examined in human plasma. Compared to human TFPI-Ig, pig TFPI-Ig inhibited pig TF activity and thrombin generation in human plasma more efficiently at certain concentrations. Conclusions: Pig TFPI-Ig will be be useful as a therapeutic protein to treat pig to human xenogeneic blood coagulation.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalBiotechnology Letters
DOIs
Publication statusAccepted/In press - 2017 Jul 26

Keywords

  • Blood coagulation
  • Recombinant protein
  • Tissue factor
  • Tissue factor pathway inhibitor
  • Xenotransplantation

ASJC Scopus subject areas

  • Biotechnology

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