Pioneer round of translation mediated by nuclear cap-binding proteins CBP80/20 occurs during prolonged hypoxia

Nara Oh, Kyoung Mi Kim, Junho Choe, Yoon Ki Kim

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Nonsense-mediated mRNA decay (NMD) is one of the mRNA surveillance mechanisms, which eliminates aberrant mRNAs harboring premature termination codons. NMD targets only mRNAs bound by the nuclear cap-binding protein complex CBP80/20 which directs the pioneer round of translation. Here we demonstrate that NMD occurs efficiently during prolonged hypoxia in which steady-state translation is drastically inhibited. Accordingly, CBP80 remains in the nucleus, and processing bodies are unaffected with regard to their abundance and number under prolonged hypoxic conditions. These results indicate that mRNAs enter the pioneer round of translation during prolonged hypoxia.

Original languageEnglish
Pages (from-to)5158-5164
Number of pages7
JournalFEBS Letters
Volume581
Issue number26
DOIs
Publication statusPublished - 2007 Oct 30

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Keywords

  • Eukaryotic translation initiation factor
  • Hypoxia
  • Nonsense-mediated mRNA decay
  • Nuclear cap-binding protein CBP80/20
  • Pioneer round of translation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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