Pioneer round of translation occurs during serum starvation

Nara Oh, Kyoung Mi Kim, Hana Cho, Junho Choe, Yoon Ki Kim

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The pioneer round of translation plays a role in translation initiation of newly spliced and exon junction complex (EJC)-bound mRNAs. Nuclear cap-binding protein complex CBP80/20 binds to those mRNAs at the 5′-end, recruiting translation initiation complex. As a consequence of the pioneer round of translation, the bound EJCs are dissociated from mRNAs and CBP80/20 is replaced by the cytoplasmic cap-binding protein eIF4E. Steady-state translation directed by eIF4E allows for an immediate and rapid response to changes in physiological conditions. Here, we show that nonsense-mediated mRNA decay (NMD), which restricts only to the pioneer round of translation but not to steady-state translation, efficiently occurs even during serum starvation, in which steady-state translation is drastically abolished. Accordingly, CBP80 remains in the nucleus and processing bodies are unaffected in their abundance and number in serum-starved conditions. These results suggest that mRNAs enter the pioneer round of translation during serum starvation and are targeted for NMD if they contain premature termination codons.

Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume362
Issue number1
DOIs
Publication statusPublished - 2007 Oct 12

Fingerprint

Starvation
Nonsense Mediated mRNA Decay
Messenger RNA
Nuclear Cap-Binding Protein Complex
Serum
RNA Cap-Binding Proteins
Nonsense Codon
Exons
Processing

Keywords

  • CBP80
  • eIF4E
  • Nonsense-mediated mRNA decay
  • Pioneer round of translation
  • Processing body
  • Serum starvation
  • Steady-state translation
  • Stress granule

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Pioneer round of translation occurs during serum starvation. / Oh, Nara; Kim, Kyoung Mi; Cho, Hana; Choe, Junho; Kim, Yoon Ki.

In: Biochemical and Biophysical Research Communications, Vol. 362, No. 1, 12.10.2007, p. 145-151.

Research output: Contribution to journalArticle

Oh, Nara ; Kim, Kyoung Mi ; Cho, Hana ; Choe, Junho ; Kim, Yoon Ki. / Pioneer round of translation occurs during serum starvation. In: Biochemical and Biophysical Research Communications. 2007 ; Vol. 362, No. 1. pp. 145-151.
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