PKCδ-dependent cleavage and nuclear translocation of annexin A1 by phorbol 12-myristate 13-acetate

Yoon S. Kim, Je Sang Ko, In S. Kim, Sung Wuk Jang, Ho J. Sung, Hye J. Lee, Si Y. Lee, Youngho Kim, Doe S. Na

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Annexin A1 (ANX-1), a calcium-dependent, phospholipid binding protein, is known to be involved in diverse cellular processes, including regulation of cell growth and differentiation, apoptosis, and inflammation. The mitogen phorbol 12-myristate 13-acetate (PMA) induces expression and phosphorylation of ANX-1. However, the roles of ANX-1 in PMA-induced signal transduction is unknown. Here, we study the cellular localization of ANX-1 in the PMA-induced signal transduction process. We have found that PMA induces the cleavage of ANX-1 in human embryonic kidney (HEK) 293 cells, and that the cleaved form of ANX-1 translocates to the nucleus. The PMA-induced nuclear translocation of ANX-1 was inhibited by the protein kinase C (PKC)δ-specific inhibitor rottlerin, indicating that PKCδ plays a role in nuclear translocation of the cleaved ANX-1. We propose a novel mechanism of PMA-induced translocation of ANX-1 to the nucleus that may participate in the regulation of cell proliferation and differentiation.

Original languageEnglish
Pages (from-to)4089-4094
Number of pages6
JournalEuropean Journal of Biochemistry
Volume270
Issue number20
DOIs
Publication statusPublished - 2003 Oct 1
Externally publishedYes

Fingerprint

Annexin A1
Annexins
Protein Kinase C
Acetates
Signal transduction
Cell Differentiation
Signal Transduction
Phosphorylation
phorbol-12-myristate
Cell proliferation
Cell growth
Mitogens
Phospholipids
Carrier Proteins
Cell Proliferation
Apoptosis
Inflammation
Calcium
Kidney

Keywords

  • Annexin A1
  • Cleavage
  • Nuclear translocation
  • PKCδ
  • PMA

ASJC Scopus subject areas

  • Biochemistry

Cite this

PKCδ-dependent cleavage and nuclear translocation of annexin A1 by phorbol 12-myristate 13-acetate. / Kim, Yoon S.; Ko, Je Sang; Kim, In S.; Jang, Sung Wuk; Sung, Ho J.; Lee, Hye J.; Lee, Si Y.; Kim, Youngho; Na, Doe S.

In: European Journal of Biochemistry, Vol. 270, No. 20, 01.10.2003, p. 4089-4094.

Research output: Contribution to journalArticle

Kim, Yoon S. ; Ko, Je Sang ; Kim, In S. ; Jang, Sung Wuk ; Sung, Ho J. ; Lee, Hye J. ; Lee, Si Y. ; Kim, Youngho ; Na, Doe S. / PKCδ-dependent cleavage and nuclear translocation of annexin A1 by phorbol 12-myristate 13-acetate. In: European Journal of Biochemistry. 2003 ; Vol. 270, No. 20. pp. 4089-4094.
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