PKR-dependent mechanisms of interferon-a for inhibiting hepatitis B virus replication

Il Hyun Park, Kyung Won Baek, Eun Young Cho, Byung-Yoon Ahn

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Interferon-a (IFN-a) inhibits the replication of hepatitis B virus (HBV) in vivo and in vitro, but the molecular mechanism of this inhibition has been elusive. We found that while HBV replication in transfected human hepatoma Huh-7 cell was severely inhibited by IFN-a treatment as reported previously, this inhibition was markedly impaired in the cell in which the expression of IFN-inducible, double- stranded RNA-dependent protein kinase (PKR) was stably and specifically suppressed through RNA-interference. Intracellular level of viral capsids was down-regulated likewise in a PKR-dependent manner, whereas that of HBV transcripts including the viral RNA pregenome was not affected by IFN-a treatment. Ectopic expression of PKR also resulted in the reduction of viral capsids with concomitant increase of phosphorylated eIF2a. These results suggested that PKR functions as a key mediator of IFN-a in opposing HBV replication, most likely through the inhibition of protein synthesis.

Original languageEnglish
Pages (from-to)167-172
Number of pages6
JournalMolecules and Cells
Volume32
Issue number2
DOIs
Publication statusPublished - 2011 Aug 1

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Virus Replication
Hepatitis B virus
Interferons
eIF-2 Kinase
Capsid
Double-Stranded RNA
Viral RNA
RNA Interference
Hepatocellular Carcinoma
Proteins

Keywords

  • Antiviral mechanism
  • Hepatitis B virus
  • IFN-a
  • PKR

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

PKR-dependent mechanisms of interferon-a for inhibiting hepatitis B virus replication. / Park, Il Hyun; Baek, Kyung Won; Cho, Eun Young; Ahn, Byung-Yoon.

In: Molecules and Cells, Vol. 32, No. 2, 01.08.2011, p. 167-172.

Research output: Contribution to journalArticle

Park, Il Hyun ; Baek, Kyung Won ; Cho, Eun Young ; Ahn, Byung-Yoon. / PKR-dependent mechanisms of interferon-a for inhibiting hepatitis B virus replication. In: Molecules and Cells. 2011 ; Vol. 32, No. 2. pp. 167-172.
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