TY - JOUR
T1 - Plasma ceruloplasmin as a biomarker for obesity
T2 - A proteomic approach
AU - Kim, Oh Yoen
AU - Shin, Min Jeong
AU - Moon, Jiyoung
AU - Chung, Ji Hyung
N1 - Funding Information:
This study was supported by a grant of the Korea Healthcare Technology R&D Project, Ministry for Health and Welfare, Republic of Korea ( A000385 and A030003 ). The authors wish to acknowledge technical support from Yonsei Proteome Research Center ( http://www.proteomix.org ).
Funding Information:
Financial support: This study was supported by a grant of the Korea Healthcare Technology R&D Project, Ministry for Health & Welfare, Republic of Korea ( A000385 and A030003 ).
Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2011/4
Y1 - 2011/4
N2 - Objectives: This study aimed to investigate new biomarkers of obesity particularly in relation with inflammation-associated proteins using protein differential display techniques. Design and methods: Comparison of protein expression in plasma between non-obese (n=109, body mass index, BMI<25kg/m2) and obese (n=32, BMI<25kg/m2) groups was carried out using two-dimensional gel electrophoresis (2-DE) analysis. ELISA was also performed for validation. Results: Among six differentially expressed protein spots, ceruloplasmin (Cp) and fibrinogen were over-expressed in obese group. Plasma Cp levels were significantly higher in obese group than non-obese group (34.0 ± 8.6 vs. 41.3 ± 12.7. mg/dL, p< 0.001) and positively correlated with age (r = 0.253, p< 0.005), BMI (r = 0.265, p< 0.001) and hsCRP (r = 0.385, p< 0.001). In stepwise multiple linear regression analysis, plasma Cp along with hsCRP were found predictors for obesity (adjusted β-coefficient. = 0.266, p< 0.01). Conclusion: Elevated plasma Cp levels were significantly associated with obesity, which may be suggested to be a marker of obesity.
AB - Objectives: This study aimed to investigate new biomarkers of obesity particularly in relation with inflammation-associated proteins using protein differential display techniques. Design and methods: Comparison of protein expression in plasma between non-obese (n=109, body mass index, BMI<25kg/m2) and obese (n=32, BMI<25kg/m2) groups was carried out using two-dimensional gel electrophoresis (2-DE) analysis. ELISA was also performed for validation. Results: Among six differentially expressed protein spots, ceruloplasmin (Cp) and fibrinogen were over-expressed in obese group. Plasma Cp levels were significantly higher in obese group than non-obese group (34.0 ± 8.6 vs. 41.3 ± 12.7. mg/dL, p< 0.001) and positively correlated with age (r = 0.253, p< 0.005), BMI (r = 0.265, p< 0.001) and hsCRP (r = 0.385, p< 0.001). In stepwise multiple linear regression analysis, plasma Cp along with hsCRP were found predictors for obesity (adjusted β-coefficient. = 0.266, p< 0.01). Conclusion: Elevated plasma Cp levels were significantly associated with obesity, which may be suggested to be a marker of obesity.
KW - Biomarker
KW - Ceruloplasmin
KW - Obesity
KW - Proteomics
KW - Two-dimensional gel electrophoresis
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U2 - 10.1016/j.clinbiochem.2011.01.014
DO - 10.1016/j.clinbiochem.2011.01.014
M3 - Article
C2 - 21291874
AN - SCOPUS:79952765244
VL - 44
SP - 351
EP - 356
JO - Clinical Biochemistry
JF - Clinical Biochemistry
SN - 0009-9120
IS - 5-6
ER -
