Plasma gel as a new augmentation material for injection laryngoplasty in rabbit model

Histologic analysis

Seok Jin Hong, Sang Hyuk Lee, Sung Min Jin, Seon Uk Lee, Se Hyung Huh, Dong Hoon Kim, Soon Young Kwon, Seung Hoon Woo

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objectives. Currently, a variety of materials are available for the treatment of glottal insufficiency. Ideal injection materials should be inexpensive, easily obtainable, nontoxic, and biocompatible. Plasma gel has recently been developed as an injectable, autologous material used in plastic surgery. The aim of this study was to evaluate the histological changes in rabbit vocal folds (VFs) after an injection of plasma gel, compared with collagen (Artecoll) or hyaluronic acid (Reviderm Intra). Study Design. Experimental prospective animal study. Setting. Animal laboratory. Subjects and Methods. Blood samples were collected from 12 New Zealand rabbits. Plasma collected from each rabbit was centrifuged and processed using a gel heating system. All rabbits received a 0.05 ml injection of plasma gel into the right VF, while an equivalent volume of Artecoll or Reviderm Intra was injected into the left VFs of each 6 rabbits randomly. The larynges were collected 2, 4, and 8 weeks after injection, and the tissues were stained for histological analysis. Results. In comparison with left VFs injected with Artecoll or Reviderm Intra, there was significantly less inflammatory response and foreign body reaction in the plasma gel injected right VFs (P ≤ .05). Multinucleated foreign body type giant cells were also more prevalent in the left VFs. There were no quantitative differences in the rates of neovascularization or collagen deposition between groups. Conclusion. Plasma gel is a biologically compatible material that may serve as a suitable augmentation material in injection laryngoplasty. Further studies that examine the longterm effects in a larger number of subjects are needed.

Original languageEnglish
Pages (from-to)596-602
Number of pages7
JournalOtolaryngology - Head and Neck Surgery (United States)
Volume149
Issue number4
DOIs
Publication statusPublished - 2013 Oct 1

Fingerprint

Laryngoplasty
Vocal Cords
Gels
Rabbits
Injections
Collagen
Foreign Body Giant Cells
Foreign-Body Reaction
Laboratory Animals
Hyaluronic Acid
Plastic Surgery
Larynx
Heating
Prospective Studies

Keywords

  • histology
  • laryngoplasty
  • plasma
  • rabbit
  • vocal fold

ASJC Scopus subject areas

  • Surgery
  • Otorhinolaryngology

Cite this

Plasma gel as a new augmentation material for injection laryngoplasty in rabbit model : Histologic analysis. / Hong, Seok Jin; Lee, Sang Hyuk; Jin, Sung Min; Lee, Seon Uk; Huh, Se Hyung; Kim, Dong Hoon; Kwon, Soon Young; Woo, Seung Hoon.

In: Otolaryngology - Head and Neck Surgery (United States), Vol. 149, No. 4, 01.10.2013, p. 596-602.

Research output: Contribution to journalArticle

Hong, Seok Jin ; Lee, Sang Hyuk ; Jin, Sung Min ; Lee, Seon Uk ; Huh, Se Hyung ; Kim, Dong Hoon ; Kwon, Soon Young ; Woo, Seung Hoon. / Plasma gel as a new augmentation material for injection laryngoplasty in rabbit model : Histologic analysis. In: Otolaryngology - Head and Neck Surgery (United States). 2013 ; Vol. 149, No. 4. pp. 596-602.
@article{4471019b3a7143fe91a31f41427fe516,
title = "Plasma gel as a new augmentation material for injection laryngoplasty in rabbit model: Histologic analysis",
abstract = "Objectives. Currently, a variety of materials are available for the treatment of glottal insufficiency. Ideal injection materials should be inexpensive, easily obtainable, nontoxic, and biocompatible. Plasma gel has recently been developed as an injectable, autologous material used in plastic surgery. The aim of this study was to evaluate the histological changes in rabbit vocal folds (VFs) after an injection of plasma gel, compared with collagen (Artecoll) or hyaluronic acid (Reviderm Intra). Study Design. Experimental prospective animal study. Setting. Animal laboratory. Subjects and Methods. Blood samples were collected from 12 New Zealand rabbits. Plasma collected from each rabbit was centrifuged and processed using a gel heating system. All rabbits received a 0.05 ml injection of plasma gel into the right VF, while an equivalent volume of Artecoll or Reviderm Intra was injected into the left VFs of each 6 rabbits randomly. The larynges were collected 2, 4, and 8 weeks after injection, and the tissues were stained for histological analysis. Results. In comparison with left VFs injected with Artecoll or Reviderm Intra, there was significantly less inflammatory response and foreign body reaction in the plasma gel injected right VFs (P ≤ .05). Multinucleated foreign body type giant cells were also more prevalent in the left VFs. There were no quantitative differences in the rates of neovascularization or collagen deposition between groups. Conclusion. Plasma gel is a biologically compatible material that may serve as a suitable augmentation material in injection laryngoplasty. Further studies that examine the longterm effects in a larger number of subjects are needed.",
keywords = "histology, laryngoplasty, plasma, rabbit, vocal fold",
author = "Hong, {Seok Jin} and Lee, {Sang Hyuk} and Jin, {Sung Min} and Lee, {Seon Uk} and Huh, {Se Hyung} and Kim, {Dong Hoon} and Kwon, {Soon Young} and Woo, {Seung Hoon}",
year = "2013",
month = "10",
day = "1",
doi = "10.1177/0194599813495664",
language = "English",
volume = "149",
pages = "596--602",
journal = "Otolaryngology - Head and Neck Surgery (United States)",
issn = "0194-5998",
publisher = "Mosby Inc.",
number = "4",

}

TY - JOUR

T1 - Plasma gel as a new augmentation material for injection laryngoplasty in rabbit model

T2 - Histologic analysis

AU - Hong, Seok Jin

AU - Lee, Sang Hyuk

AU - Jin, Sung Min

AU - Lee, Seon Uk

AU - Huh, Se Hyung

AU - Kim, Dong Hoon

AU - Kwon, Soon Young

AU - Woo, Seung Hoon

PY - 2013/10/1

Y1 - 2013/10/1

N2 - Objectives. Currently, a variety of materials are available for the treatment of glottal insufficiency. Ideal injection materials should be inexpensive, easily obtainable, nontoxic, and biocompatible. Plasma gel has recently been developed as an injectable, autologous material used in plastic surgery. The aim of this study was to evaluate the histological changes in rabbit vocal folds (VFs) after an injection of plasma gel, compared with collagen (Artecoll) or hyaluronic acid (Reviderm Intra). Study Design. Experimental prospective animal study. Setting. Animal laboratory. Subjects and Methods. Blood samples were collected from 12 New Zealand rabbits. Plasma collected from each rabbit was centrifuged and processed using a gel heating system. All rabbits received a 0.05 ml injection of plasma gel into the right VF, while an equivalent volume of Artecoll or Reviderm Intra was injected into the left VFs of each 6 rabbits randomly. The larynges were collected 2, 4, and 8 weeks after injection, and the tissues were stained for histological analysis. Results. In comparison with left VFs injected with Artecoll or Reviderm Intra, there was significantly less inflammatory response and foreign body reaction in the plasma gel injected right VFs (P ≤ .05). Multinucleated foreign body type giant cells were also more prevalent in the left VFs. There were no quantitative differences in the rates of neovascularization or collagen deposition between groups. Conclusion. Plasma gel is a biologically compatible material that may serve as a suitable augmentation material in injection laryngoplasty. Further studies that examine the longterm effects in a larger number of subjects are needed.

AB - Objectives. Currently, a variety of materials are available for the treatment of glottal insufficiency. Ideal injection materials should be inexpensive, easily obtainable, nontoxic, and biocompatible. Plasma gel has recently been developed as an injectable, autologous material used in plastic surgery. The aim of this study was to evaluate the histological changes in rabbit vocal folds (VFs) after an injection of plasma gel, compared with collagen (Artecoll) or hyaluronic acid (Reviderm Intra). Study Design. Experimental prospective animal study. Setting. Animal laboratory. Subjects and Methods. Blood samples were collected from 12 New Zealand rabbits. Plasma collected from each rabbit was centrifuged and processed using a gel heating system. All rabbits received a 0.05 ml injection of plasma gel into the right VF, while an equivalent volume of Artecoll or Reviderm Intra was injected into the left VFs of each 6 rabbits randomly. The larynges were collected 2, 4, and 8 weeks after injection, and the tissues were stained for histological analysis. Results. In comparison with left VFs injected with Artecoll or Reviderm Intra, there was significantly less inflammatory response and foreign body reaction in the plasma gel injected right VFs (P ≤ .05). Multinucleated foreign body type giant cells were also more prevalent in the left VFs. There were no quantitative differences in the rates of neovascularization or collagen deposition between groups. Conclusion. Plasma gel is a biologically compatible material that may serve as a suitable augmentation material in injection laryngoplasty. Further studies that examine the longterm effects in a larger number of subjects are needed.

KW - histology

KW - laryngoplasty

KW - plasma

KW - rabbit

KW - vocal fold

UR - http://www.scopus.com/inward/record.url?scp=84884302060&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884302060&partnerID=8YFLogxK

U2 - 10.1177/0194599813495664

DO - 10.1177/0194599813495664

M3 - Article

VL - 149

SP - 596

EP - 602

JO - Otolaryngology - Head and Neck Surgery (United States)

JF - Otolaryngology - Head and Neck Surgery (United States)

SN - 0194-5998

IS - 4

ER -