Plasma proteomic analysis of patients infected with H1N1 influenza virus

Seonyoung Choi, Ji youn Lim, Yoonjin Kim, Moon Jung Song, Woon Won Jung, Jung Bok Seo, Jacob Lee, Dong Geun Sul

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This study profiled the plasma proteins of patients infected by the 2011 H1N1 influenza virus. Differential protein expression was identified in plasma obtained from noninfected control subjects (n = 15) and H1N1-infected subjects (n = 15). Plasma proteins were separated by a 2DE large gel system and identified by nano-ultra performance LC-MS. Western blot assays were performed to validate proteins. Eight plasma proteins were upregulated and six proteins were downregulated among 3316 plasma proteins in the H1N1-infected group as compared with the control group. Of 14 up- and downregulated proteins, nine plasma proteins were validated by Western blot analysis. Putative protein FAM 157A, leucine-rich alpha 2 glycoprotein, serum amyloid A protein, and dual oxidase 1 showed significant differential expression. The identified plasma proteins could be potential candidates for biomarkers of H1N1 influenza viral infection. Further studies are needed to develop these proteins as diagnostic biomarkers.

Original languageEnglish
Pages (from-to)1933-1942
Number of pages10
JournalProteomics
Volume14
Issue number16
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

H1N1 Subtype Influenza A Virus
Orthomyxoviridae
Viruses
Proteomics
Blood Proteins
Plasmas
Proteins
Biomarkers
Down-Regulation
Western Blotting
Serum Amyloid A Protein
Virus Diseases
Leucine
Human Influenza
Assays
Glycoproteins
Oxidoreductases
Gels
Control Groups

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Plasma proteomic analysis of patients infected with H1N1 influenza virus. / Choi, Seonyoung; Lim, Ji youn; Kim, Yoonjin; Song, Moon Jung; Jung, Woon Won; Seo, Jung Bok; Lee, Jacob; Sul, Dong Geun.

In: Proteomics, Vol. 14, No. 16, 01.01.2014, p. 1933-1942.

Research output: Contribution to journalArticle

Choi, S, Lim, JY, Kim, Y, Song, MJ, Jung, WW, Seo, JB, Lee, J & Sul, DG 2014, 'Plasma proteomic analysis of patients infected with H1N1 influenza virus', Proteomics, vol. 14, no. 16, pp. 1933-1942. https://doi.org/10.1002/pmic.201400030
Choi, Seonyoung ; Lim, Ji youn ; Kim, Yoonjin ; Song, Moon Jung ; Jung, Woon Won ; Seo, Jung Bok ; Lee, Jacob ; Sul, Dong Geun. / Plasma proteomic analysis of patients infected with H1N1 influenza virus. In: Proteomics. 2014 ; Vol. 14, No. 16. pp. 1933-1942.
@article{91ecfbbd9fa34fcb85cf19ecbc8086f7,
title = "Plasma proteomic analysis of patients infected with H1N1 influenza virus",
abstract = "This study profiled the plasma proteins of patients infected by the 2011 H1N1 influenza virus. Differential protein expression was identified in plasma obtained from noninfected control subjects (n = 15) and H1N1-infected subjects (n = 15). Plasma proteins were separated by a 2DE large gel system and identified by nano-ultra performance LC-MS. Western blot assays were performed to validate proteins. Eight plasma proteins were upregulated and six proteins were downregulated among 3316 plasma proteins in the H1N1-infected group as compared with the control group. Of 14 up- and downregulated proteins, nine plasma proteins were validated by Western blot analysis. Putative protein FAM 157A, leucine-rich alpha 2 glycoprotein, serum amyloid A protein, and dual oxidase 1 showed significant differential expression. The identified plasma proteins could be potential candidates for biomarkers of H1N1 influenza viral infection. Further studies are needed to develop these proteins as diagnostic biomarkers.",
keywords = "Biomarkers, Biomedicine, H1N1, Influenza virus, Plasma proteins",
author = "Seonyoung Choi and Lim, {Ji youn} and Yoonjin Kim and Song, {Moon Jung} and Jung, {Woon Won} and Seo, {Jung Bok} and Jacob Lee and Sul, {Dong Geun}",
year = "2014",
month = "1",
day = "1",
doi = "10.1002/pmic.201400030",
language = "English",
volume = "14",
pages = "1933--1942",
journal = "Proteomics",
issn = "1615-9853",
publisher = "Wiley-VCH Verlag",
number = "16",

}

TY - JOUR

T1 - Plasma proteomic analysis of patients infected with H1N1 influenza virus

AU - Choi, Seonyoung

AU - Lim, Ji youn

AU - Kim, Yoonjin

AU - Song, Moon Jung

AU - Jung, Woon Won

AU - Seo, Jung Bok

AU - Lee, Jacob

AU - Sul, Dong Geun

PY - 2014/1/1

Y1 - 2014/1/1

N2 - This study profiled the plasma proteins of patients infected by the 2011 H1N1 influenza virus. Differential protein expression was identified in plasma obtained from noninfected control subjects (n = 15) and H1N1-infected subjects (n = 15). Plasma proteins were separated by a 2DE large gel system and identified by nano-ultra performance LC-MS. Western blot assays were performed to validate proteins. Eight plasma proteins were upregulated and six proteins were downregulated among 3316 plasma proteins in the H1N1-infected group as compared with the control group. Of 14 up- and downregulated proteins, nine plasma proteins were validated by Western blot analysis. Putative protein FAM 157A, leucine-rich alpha 2 glycoprotein, serum amyloid A protein, and dual oxidase 1 showed significant differential expression. The identified plasma proteins could be potential candidates for biomarkers of H1N1 influenza viral infection. Further studies are needed to develop these proteins as diagnostic biomarkers.

AB - This study profiled the plasma proteins of patients infected by the 2011 H1N1 influenza virus. Differential protein expression was identified in plasma obtained from noninfected control subjects (n = 15) and H1N1-infected subjects (n = 15). Plasma proteins were separated by a 2DE large gel system and identified by nano-ultra performance LC-MS. Western blot assays were performed to validate proteins. Eight plasma proteins were upregulated and six proteins were downregulated among 3316 plasma proteins in the H1N1-infected group as compared with the control group. Of 14 up- and downregulated proteins, nine plasma proteins were validated by Western blot analysis. Putative protein FAM 157A, leucine-rich alpha 2 glycoprotein, serum amyloid A protein, and dual oxidase 1 showed significant differential expression. The identified plasma proteins could be potential candidates for biomarkers of H1N1 influenza viral infection. Further studies are needed to develop these proteins as diagnostic biomarkers.

KW - Biomarkers

KW - Biomedicine

KW - H1N1

KW - Influenza virus

KW - Plasma proteins

UR - http://www.scopus.com/inward/record.url?scp=84905691997&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84905691997&partnerID=8YFLogxK

U2 - 10.1002/pmic.201400030

DO - 10.1002/pmic.201400030

M3 - Article

C2 - 24888898

AN - SCOPUS:84905691997

VL - 14

SP - 1933

EP - 1942

JO - Proteomics

JF - Proteomics

SN - 1615-9853

IS - 16

ER -