Plasma proteomic analysis of patients infected with H1N1 influenza virus

Seonyoung Choi, Ji youn Lim, Yoonjin Kim, Moon Jung Song, Woon Won Jung, Jung Bok Seo, Jacob Lee, Dong Geun Sul

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

This study profiled the plasma proteins of patients infected by the 2011 H1N1 influenza virus. Differential protein expression was identified in plasma obtained from noninfected control subjects (n = 15) and H1N1-infected subjects (n = 15). Plasma proteins were separated by a 2DE large gel system and identified by nano-ultra performance LC-MS. Western blot assays were performed to validate proteins. Eight plasma proteins were upregulated and six proteins were downregulated among 3316 plasma proteins in the H1N1-infected group as compared with the control group. Of 14 up- and downregulated proteins, nine plasma proteins were validated by Western blot analysis. Putative protein FAM 157A, leucine-rich alpha 2 glycoprotein, serum amyloid A protein, and dual oxidase 1 showed significant differential expression. The identified plasma proteins could be potential candidates for biomarkers of H1N1 influenza viral infection. Further studies are needed to develop these proteins as diagnostic biomarkers.

Original languageEnglish
Pages (from-to)1933-1942
Number of pages10
JournalProteomics
Volume14
Issue number16
DOIs
Publication statusPublished - 2014 Jan 1

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ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

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