Plasmid vectors harboring cellular promoters can induce prolonged gene expression in hematopoietic and mesenchymal progenitor cells

Hyang Min Byun, Dongchul Suh, Youngsin Jeong, Seok Wee Hyung, Mogg Kim Jung, Won Ki Kim, Jae Ko Jung, Jin Seok Kim, Bok Lee Yong, Yu Kyoung Oh

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Although prolonged transgene expression in progenitor cells might be desirable for modified cell therapy, the viral promoter-based expression vector tends to promote transgene expression only for a limited period. Here, we examined the ability of cellular promoters from elongation factor-1α (EF-1α) and ubiquitin C to drive gene expression in hematopoietic TF-1 and mesenchymal progenitor cells. We compared the expression levels and duration of a model gene, interleukin-2, generated by the cellular promoters to those by the cytomegalovirus (CMV) promoter. The EF-1α and ubiquitin C promoters drove prolonged gene expression in hematopoietic TF-1 and mesenchymal progenitor cells, whereas the CMV promoter did not. At day 7 after transfection in TF-1 cells, the mRNA expression levels of interleukin-2 driven by the EF-1α and ubiquitin C promoters were 118- and 56-fold higher, respectively, than those driven by the CMV promoter. Similarly, in mesenchymal progenitor cells, the expression levels of interleukin-2 driven by the EF-1α and ubiquitin C promoters were 98- and 20-fold higher, respectively, than that driven by the CMV promoter-encoding plasmid. Moreover, the ubiquitin C promoter directed higher levels of green fluorescence protein expression in mesenchymal progenitor cells than did the CMV promoter. These results indicate that the use of cellular promoters such as those for EF-1α and ubiquitin C might direct prolonged gene expression in hematopoietic and mesenchymal progenitor cells.

Original languageEnglish
Pages (from-to)518-523
Number of pages6
JournalBiochemical and biophysical research communications
Volume332
Issue number2
DOIs
Publication statusPublished - 2005 Jul 1
Externally publishedYes

Keywords

  • Elongation factor-1α
  • Hematopoietic cells
  • Mesenchymal progenitor cells
  • Promoters
  • Ubiquitin C

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Plasmid vectors harboring cellular promoters can induce prolonged gene expression in hematopoietic and mesenchymal progenitor cells'. Together they form a unique fingerprint.

  • Cite this