Plastic microchip flow cytometer based on 2- and 3-dimensional hydrodynamic flow focusing

S. Chung; S. J. Park; J. K. Kim; C. Chung; D. C. Han; J. K. Chang

doi:10.1007/s00542-003-0302-2

Plastic microchip flow cytometer based on 2- and 3-dimensional hydrodynamic flow focusing

S. Chung, S. J. Park, J. K. Kim, C. Chung, D. C. Han, J. K. Chang

School of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

63 Citations (Scopus)

Abstract

This paper deals with design and fabrication of 2- and 3- dimensional microchip type flow cytometers hydrodynamically driven. Flow rate and pressure distrubution have been calculated from Poiseuille flow theory and results were confirmed by computational fluid dynamics. The flow focusing has been evaluated in 2- and 3-dimensional microchip type flow cytometers fabricated with Polydimetylsiloxane molding process. The shape of focused flow was detected with a laser scanning microscope and favourably compared with calculations based on Poiseuille flow assumptions and CFD (Computational Fluid Dynamics) simulation. Simulation and calculation results showed good correlations to each other, and also to the visualized shape of focused flow in microchip. The 3-dimensional focused flow showed the more stable focusing status in laser induced fluorescence measurements than that in 2-dimensional case.

Original language	English
Pages (from-to)	525-533
Number of pages	9
Journal	Microsystem Technologies
Volume	9
Issue number	8
DOIs	https://doi.org/10.1007/s00542-003-0302-2
Publication status	Published - 2003 Oct

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Hardware and Architecture
Electrical and Electronic Engineering

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10.1007/s00542-003-0302-2

Cite this

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abstract = "This paper deals with design and fabrication of 2- and 3- dimensional microchip type flow cytometers hydrodynamically driven. Flow rate and pressure distrubution have been calculated from Poiseuille flow theory and results were confirmed by computational fluid dynamics. The flow focusing has been evaluated in 2- and 3-dimensional microchip type flow cytometers fabricated with Polydimetylsiloxane molding process. The shape of focused flow was detected with a laser scanning microscope and favourably compared with calculations based on Poiseuille flow assumptions and CFD (Computational Fluid Dynamics) simulation. Simulation and calculation results showed good correlations to each other, and also to the visualized shape of focused flow in microchip. The 3-dimensional focused flow showed the more stable focusing status in laser induced fluorescence measurements than that in 2-dimensional case.",

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AU - Chung, S.

AU - Park, S. J.

AU - Kim, J. K.

AU - Chung, C.

AU - Han, D. C.

AU - Chang, J. K.

PY - 2003/10

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AB - This paper deals with design and fabrication of 2- and 3- dimensional microchip type flow cytometers hydrodynamically driven. Flow rate and pressure distrubution have been calculated from Poiseuille flow theory and results were confirmed by computational fluid dynamics. The flow focusing has been evaluated in 2- and 3-dimensional microchip type flow cytometers fabricated with Polydimetylsiloxane molding process. The shape of focused flow was detected with a laser scanning microscope and favourably compared with calculations based on Poiseuille flow assumptions and CFD (Computational Fluid Dynamics) simulation. Simulation and calculation results showed good correlations to each other, and also to the visualized shape of focused flow in microchip. The 3-dimensional focused flow showed the more stable focusing status in laser induced fluorescence measurements than that in 2-dimensional case.

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Plastic microchip flow cytometer based on 2- and 3-dimensional hydrodynamic flow focusing

Abstract

ASJC Scopus subject areas

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