Pluronic/Heparin Nanoparticles for Chemo-Photodynamic Combination Cancer Therapy through Photoinduced Caspase-3 Activation

Nisar Ul Khaliq, Dal Yong Park, Hye Jin Lee, Keun Sang Oh, Jae Hong Seo, Sang Yoon Kim, Chang Soon Hwang, Tae Hong Lim, Soon Hong Yuk

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The Pluronic/heparin nanoparticles (NPs) were prepared for chemo-photodynamic combination cancer therapy through photoinduced caspase-3 activation. Doxorubicin (DOX) prodrug and methylene blue (MB) were assembled into a single structure by electrostatic interaction with heparin and further loaded into the NPs. MB is a photosensitizer, which can be used for photodynamic therapy, which can elicit cell death by producing reactive oxygen species (ROS). The DOX prodrug was DOX conjugated to a labile tetrapeptide (DEVD) by caspase-3 upon apoptosis. ROS was measured to verify ROS-mediated apoptosis. Membrane integrity test and flow cytometry analysis were performed to examine induced apoptosis. The tumor targeting and the in vivo antitumor efficacy were measured to evaluate chemo-photodynamic combination cancer therapy in tumor-bearing mice.

Original languageEnglish
Pages (from-to)2943-2952
Number of pages10
JournalACS Applied Nano Materials
Volume1
Issue number6
DOIs
Publication statusPublished - 2018 Jun 22

Keywords

  • apoptosis
  • combination therapy
  • photodynamic therapy
  • Pluronic/heparin nanoparticles
  • prodrug

ASJC Scopus subject areas

  • Materials Science(all)

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