Polarized and stage-dependent distribution of immunoreactivity for novel PDZ-binding protein preso1 in adult neurogenic regions

Eun Soo Lee, Woon Ryoung Kim, Younghwa Kim, Hyun Woo Lee, Hyun Kim, Woong Sun

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: Adult neural stem cells have the potential for self-renewal and differentiation into multiple cell lineages via symmetric or asymmetric cell division. Preso1 is a recently identified protein involved in the formation of dendritic spines and the promotion of axonal growth in developing neurons. Preso1 can also bind to cell polarity proteins, suggesting a potential role for Preso1 in asymmetric cell division. Methods: To investigate the distribution of Preso1, we performed immunohistochemistry with adult mouse brain slice. Also, polarized distribution of Preso1 was assessed by immunocytochemistry in cultured neural stem cells. Results: Immunoreactivity for Preso1 (Preso1-IR) was strong in the rostral migratory stream and subventricular zone, where proliferating transit-amplifying cells and neuroblasts are prevalent. In cultured neural stem cells, Preso1-IR was unequally distributed in the cell cytosol. We also observed the distribution of Preso1 in the subgranular zone of the hippocampal dentate gyrus, another neurogenic region in the adult brain. Interestingly, Preso1-IR was transiently observed in the nuclei of doublecortin-expressing neuroblasts immediately after asymmetric cell division. Conclusion: Our study demonstrated that Preso1 is asymmetrically distributed in the cytosol and nuclei of neural stem/progenitor cells in the adult brain, and may play a significant role in cell differentiation via association with cell polarity machinery.

Original languageEnglish
Pages (from-to)349-355
Number of pages7
JournalEndocrinology and Metabolism
Volume29
Issue number3
DOIs
Publication statusPublished - 2014

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Neural Stem Cells
Asymmetric Cell Division
Carrier Proteins
Cell Polarity
Cytosol
Brain
Immunohistochemistry
Parahippocampal Gyrus
Dendritic Spines
Adult Stem Cells
Lateral Ventricles
Dentate Gyrus
Cell Lineage
Cell Differentiation
Proteins
Stem Cells
Neurons
Growth

Keywords

  • Adult neural stem cells
  • Cell polarity
  • Dentate gyrus
  • Frmpd4
  • Preso1
  • Subventricular zone

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Polarized and stage-dependent distribution of immunoreactivity for novel PDZ-binding protein preso1 in adult neurogenic regions. / Lee, Eun Soo; Kim, Woon Ryoung; Kim, Younghwa; Lee, Hyun Woo; Kim, Hyun; Sun, Woong.

In: Endocrinology and Metabolism, Vol. 29, No. 3, 2014, p. 349-355.

Research output: Contribution to journalArticle

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AB - Background: Adult neural stem cells have the potential for self-renewal and differentiation into multiple cell lineages via symmetric or asymmetric cell division. Preso1 is a recently identified protein involved in the formation of dendritic spines and the promotion of axonal growth in developing neurons. Preso1 can also bind to cell polarity proteins, suggesting a potential role for Preso1 in asymmetric cell division. Methods: To investigate the distribution of Preso1, we performed immunohistochemistry with adult mouse brain slice. Also, polarized distribution of Preso1 was assessed by immunocytochemistry in cultured neural stem cells. Results: Immunoreactivity for Preso1 (Preso1-IR) was strong in the rostral migratory stream and subventricular zone, where proliferating transit-amplifying cells and neuroblasts are prevalent. In cultured neural stem cells, Preso1-IR was unequally distributed in the cell cytosol. We also observed the distribution of Preso1 in the subgranular zone of the hippocampal dentate gyrus, another neurogenic region in the adult brain. Interestingly, Preso1-IR was transiently observed in the nuclei of doublecortin-expressing neuroblasts immediately after asymmetric cell division. Conclusion: Our study demonstrated that Preso1 is asymmetrically distributed in the cytosol and nuclei of neural stem/progenitor cells in the adult brain, and may play a significant role in cell differentiation via association with cell polarity machinery.

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