Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment

Che O. Jeon, Woojun Park, William C. Ghiorse, Eugene L. Madsen

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Strain CJ2T, capable of growth on naphthalene as a sole carbon and energy source, was isolated from coal-tar-contaminated freshwater sediment. The Gram reaction of strain CJ2T was negative. The cells were non-spore-forming, non-motile cocci (without flagella). The isolate was found to be an aerobic heterotroph capable of utilizing glucose and other simple sugars. Growth was observed between 4 and 25 °C (optimum, 20 °C) and between pH 6.0 and 9.0 (optimum, pH 7.0-7.5). The G+C content of the genomic DNA was 61.5 mol% and the major quinone was ubiquinone-8. The peptidoglycan of strain CJ2T was determined as belonging to type A1-γ, meso-diaminopimelic acid. The major fatty acids of strain CJ2T were 16:1ω7c (67-0%), 16:0 (19.6%),18:1ω7c (∼7.9%) and 10:03-OH (∼2.5%). The polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Mycolic acid and glycolipids could not be detected. Comparative 16S rDNA analysis indicated that strain CJ2T is related to the family Comamonadaceae and that the nearest phylogenetic relative was Polaromonas vacuolata 34-PT (97.1% similarity). On the basis of the physiological and molecular properties, the naphthalene-degrading isolate was designated Polaromonas naphthalenivorans sp. nov. The type strain is CJ2T (=ATCC BAA-779T= DSM 15660T).

Original languageEnglish
Pages (from-to)93-97
Number of pages5
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume54
Issue number1
DOIs
Publication statusPublished - 2004 Jan 1
Externally publishedYes

Fingerprint

Polaromonas
naphthalene
Bacteria
Sediments
Comamonadaceae
Coal Tar
Mycolic Acids
Diaminopimelic Acid
coal tar
sediments
Phosphatidylglycerols
freshwater sediment
bacterium
Cardiolipins
Flagella
Peptidoglycan
new species
acid
Glycolipids
bacteria

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology
  • Immunology
  • Microbiology

Cite this

Polaromonas naphthalenivorans sp. nov., a naphthalene-degrading bacterium from naphthalene-contaminated sediment. / Jeon, Che O.; Park, Woojun; Ghiorse, William C.; Madsen, Eugene L.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 54, No. 1, 01.01.2004, p. 93-97.

Research output: Contribution to journalArticle

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