TY - JOUR
T1 - Poly-γ-glutamic acid enhances the growth and viability of Chinese hamster ovary cells in serum-free medium.
AU - Chun, Bok Hwan
AU - Lee, Yong Kwon
AU - Chung, Namhyun
N1 - Funding Information:
This study was supported by Korea Sanhak Foundation, Republic of Korea.
PY - 2012/10
Y1 - 2012/10
N2 - The protective effects of polymer additives, including a group of viscosity-enhancing polymer poly-γ-glutamic acid (γPGA; 10, 50, and 500 kDa) and surface-active polymer Pluronic F68, on Chinese hamster ovary cells against damage due to shear stress were investigated in shake-flask cultures. The level of protection was dependent upon the molecular weight of γPGA and its concentration. When 0.05 or 0.075 % of 500 kDa γPGA was added, the cell growth and viability were almost equal to those of Pluronic F68 supplementation and were much higher than those of the control without additives. For the first time, we show that γPGA is another environmentally-friendly medium additive that can be used in place of Pluronic F68.
AB - The protective effects of polymer additives, including a group of viscosity-enhancing polymer poly-γ-glutamic acid (γPGA; 10, 50, and 500 kDa) and surface-active polymer Pluronic F68, on Chinese hamster ovary cells against damage due to shear stress were investigated in shake-flask cultures. The level of protection was dependent upon the molecular weight of γPGA and its concentration. When 0.05 or 0.075 % of 500 kDa γPGA was added, the cell growth and viability were almost equal to those of Pluronic F68 supplementation and were much higher than those of the control without additives. For the first time, we show that γPGA is another environmentally-friendly medium additive that can be used in place of Pluronic F68.
UR - http://www.scopus.com/inward/record.url?scp=85027936259&partnerID=8YFLogxK
U2 - 10.1007/s10529-012-0982-8
DO - 10.1007/s10529-012-0982-8
M3 - Article
C2 - 22714280
AN - SCOPUS:85027936259
SN - 0141-5492
VL - 34
SP - 1807
EP - 1810
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 10
ER -