Poly-γ-glutamic acid enhances the growth and viability of Chinese hamster ovary cells in serum-free medium.

Bok Hwan Chun, Yong Kwon Lee, Namhyun Chung

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The protective effects of polymer additives, including a group of viscosity-enhancing polymer poly-γ-glutamic acid (γPGA; 10, 50, and 500 kDa) and surface-active polymer Pluronic F68, on Chinese hamster ovary cells against damage due to shear stress were investigated in shake-flask cultures. The level of protection was dependent upon the molecular weight of γPGA and its concentration. When 0.05 or 0.075 % of 500 kDa γPGA was added, the cell growth and viability were almost equal to those of Pluronic F68 supplementation and were much higher than those of the control without additives. For the first time, we show that γPGA is another environmentally-friendly medium additive that can be used in place of Pluronic F68.

Original languageEnglish
Pages (from-to)1807-1810
Number of pages4
JournalBiotechnology Letters
Volume34
Issue number10
Publication statusPublished - 2012

Fingerprint

Prostaglandins A
Serum-Free Culture Media
Cricetulus
Poloxamer
Glutamic Acid
Ovary
Polymers
Growth
Batch Cell Culture Techniques
Viscosity
Cell Survival
Molecular Weight

ASJC Scopus subject areas

  • Biotechnology

Cite this

Poly-γ-glutamic acid enhances the growth and viability of Chinese hamster ovary cells in serum-free medium. / Chun, Bok Hwan; Lee, Yong Kwon; Chung, Namhyun.

In: Biotechnology Letters, Vol. 34, No. 10, 2012, p. 1807-1810.

Research output: Contribution to journalArticle

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