TY - JOUR
T1 - Poly-γ-glutamic acid suppresses osteoclastogenesis in human osteoclast precursors and prevents joint damage in a collagen-induced murine arthritis model
AU - Lee, Bitnara
AU - Jo, Sungsin
AU - Kim, Sung Min
AU - Cho, Mi La
AU - Park, Sung Hwan
AU - Youn, Jeehee
AU - Ji, Jong Dae
AU - Kim, Tae Hwan
PY - 2018/11/1
Y1 - 2018/11/1
N2 - Poly–γ-glutamic acid (γ-PGA), a natural polymer derived from Bacillus subtilis, shows anti-inflammatory activity. However, the effects of γ-PGA on osteoclasts, which are important cells for joint destruction in inflammatory diseases such as rheumatoid arthritis (RA), have not yet been reported. In this study, we show that γ-PGA markedly inhibits osteoclast differentiation in normal PBMC-derived osteoclast precursors and in synovial fluid macrophages of patients with RA. γ-PGA also reduces RANK expression by down-regulating M-CSF receptors. Additionally, oral administration of γ-PGA attenuated bone destruction in a collagen-induced arthritis (CIA) model, demonstrating decreases in inflammation, cartilage damage, and osteoclast formation in histological analyses. Taken together, these data suggest that γ-PGA could be a good candidate for therapeutic prevention of joint destruction in RA.
AB - Poly–γ-glutamic acid (γ-PGA), a natural polymer derived from Bacillus subtilis, shows anti-inflammatory activity. However, the effects of γ-PGA on osteoclasts, which are important cells for joint destruction in inflammatory diseases such as rheumatoid arthritis (RA), have not yet been reported. In this study, we show that γ-PGA markedly inhibits osteoclast differentiation in normal PBMC-derived osteoclast precursors and in synovial fluid macrophages of patients with RA. γ-PGA also reduces RANK expression by down-regulating M-CSF receptors. Additionally, oral administration of γ-PGA attenuated bone destruction in a collagen-induced arthritis (CIA) model, demonstrating decreases in inflammation, cartilage damage, and osteoclast formation in histological analyses. Taken together, these data suggest that γ-PGA could be a good candidate for therapeutic prevention of joint destruction in RA.
KW - Osteoclast
KW - Poly-γ-glutamic acid (γ -PGA)
KW - Rheumatoid arthritis
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U2 - 10.1016/j.imlet.2018.09.004
DO - 10.1016/j.imlet.2018.09.004
M3 - Article
C2 - 30213687
AN - SCOPUS:85054067329
VL - 203
SP - 80
EP - 86
JO - Immunology Letters
JF - Immunology Letters
SN - 0165-2478
ER -