Poly(dimethylsiloxane)-based micro chamber with air permeable cover sheet for the protoplast of Nicotiana tabacum culture

Jongil Ju, Jung Moon Ko, Hyeon Cheol Cha, Sang Hoon Lee

Research output: Contribution to journalConference article

Abstract

In plant cell culture, the delivery of nutrition and gas (mainly oxygen) to the cells is the most important factor for viability. In this paper, we propose a poly(dimethylsiloxane) (PDMS)-based micro culture system that is designed to have good aeration. PDMS is known to have excellent air permeability, and through the experimental method, we investigated the relation between the degree of air delivery and the thickness of the PDMS sheet covering the culture chamber. We determined the proper thickness of the cover sheet, and cultured protoplasts of Nicotiana tabacum in a culture chamber covered with a PDMS sheet whose thickness 400 μm. The cells were successfully divided, and lived well inside the culture chamber for 10 days. In addition, protoplasts were cultured inside the culture chambers covered with the coverglass and the PDMS sheet respectively, and the microcolonies were formed well inside the PDMS covered chamber after 10 days.

Original languageEnglish
Pages (from-to)298-301
Number of pages4
JournalIFMBE Proceedings
Volume14
Issue number1
Publication statusPublished - 2007 Jan 1
Event10th World Congress on Medical Physics and Biomedical Engineering, WC 2006 - Seoul, Korea, Republic of
Duration: 2006 Aug 272006 Sep 1

Keywords

  • Air-permeability
  • Cell-culture
  • CPW
  • Nicotiana tabacum
  • Poly(dimethylsiloxane)(PDMS)

ASJC Scopus subject areas

  • Bioengineering
  • Biomedical Engineering

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