Poly(dimethylsiloxane)-based micro chamber with air permeable cover sheet for the protoplast of Nicotiana tabacum culture

Jongil Ju, Jung Moon Ko, Hyeon Cheol Cha, Sang Hoon Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

In plant cell culture, the delivery of nutrition and gas (mainly oxygen) to the cells is the most important factor for viability. In this paper, we propose a poly(dimethylsiloxane) (PDMS)-based micro culture system that is designed to have good aeration. PDMS is known to have excellent air permeability, and through the experimental method, we investigated the relation between the degree of air delivery and the thickness of the PDMS sheet covering the culture chamber. We determined the proper thickness of the cover sheet, and cultured protoplasts of Nicotiana tabacum in a culture chamber covered with a PDMS sheet whose thickness 400 μm. The cells were successfully divided, and lived well inside the culture chamber for 10 days. In addition, protoplasts were cultured inside the culture chambers covered with the coverglass and the PDMS sheet respectively, and the microcolonies were formed well inside the PDMS covered chamber after 10 days.

Original languageEnglish
Title of host publicationIFMBE Proceedings
PublisherSpringer Verlag
Pages298-301
Number of pages4
Volume14
Edition1
Publication statusPublished - 2007
Event10th World Congress on Medical Physics and Biomedical Engineering, WC 2006 - Seoul, Korea, Republic of
Duration: 2006 Aug 272006 Sep 1

Other

Other10th World Congress on Medical Physics and Biomedical Engineering, WC 2006
CountryKorea, Republic of
CitySeoul
Period06/8/2706/9/1

Fingerprint

Polydimethylsiloxane
Cell culture
Air
Plant cell culture
Air permeability
Nutrition
baysilon
Gases
Oxygen

Keywords

  • Air-permeability
  • Cell-culture
  • CPW
  • Nicotiana tabacum
  • Poly(dimethylsiloxane)(PDMS)

ASJC Scopus subject areas

  • Biomedical Engineering
  • Bioengineering

Cite this

Ju, J., Ko, J. M., Cha, H. C., & Lee, S. H. (2007). Poly(dimethylsiloxane)-based micro chamber with air permeable cover sheet for the protoplast of Nicotiana tabacum culture. In IFMBE Proceedings (1 ed., Vol. 14, pp. 298-301). Springer Verlag.

Poly(dimethylsiloxane)-based micro chamber with air permeable cover sheet for the protoplast of Nicotiana tabacum culture. / Ju, Jongil; Ko, Jung Moon; Cha, Hyeon Cheol; Lee, Sang Hoon.

IFMBE Proceedings. Vol. 14 1. ed. Springer Verlag, 2007. p. 298-301.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ju, J, Ko, JM, Cha, HC & Lee, SH 2007, Poly(dimethylsiloxane)-based micro chamber with air permeable cover sheet for the protoplast of Nicotiana tabacum culture. in IFMBE Proceedings. 1 edn, vol. 14, Springer Verlag, pp. 298-301, 10th World Congress on Medical Physics and Biomedical Engineering, WC 2006, Seoul, Korea, Republic of, 06/8/27.
Ju J, Ko JM, Cha HC, Lee SH. Poly(dimethylsiloxane)-based micro chamber with air permeable cover sheet for the protoplast of Nicotiana tabacum culture. In IFMBE Proceedings. 1 ed. Vol. 14. Springer Verlag. 2007. p. 298-301
Ju, Jongil ; Ko, Jung Moon ; Cha, Hyeon Cheol ; Lee, Sang Hoon. / Poly(dimethylsiloxane)-based micro chamber with air permeable cover sheet for the protoplast of Nicotiana tabacum culture. IFMBE Proceedings. Vol. 14 1. ed. Springer Verlag, 2007. pp. 298-301
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