Poly(dimethylsiloxane)-based protein preconcentration using a nanogap generated by junction gap breakdown

Hoon Lee Jeong; Seok Chung; Jae Kim Sung; Jongyoon Han

doi:10.1021/ac071162h

Poly(dimethylsiloxane)-based protein preconcentration using a nanogap generated by junction gap breakdown

Hoon Lee Jeong, Seok Chung, Jae Kim Sung, Jongyoon Han

Research output: Contribution to journal › Article › peer-review

128 Citations (Scopus)

Abstract

Simple and efficient sample concentration tools are the key to the application of proteomics in a biological system. In this paper, we developed a method to realize a nanofluidic preconcentrator on a poly(dimethylsiloxane) (PDMS)-based microfluidic channel. The originality of our preconcentration device is the simple nanogap formation using the junction gap breakdown phenomenon between two PDMS microchannels, without using any photolithography and etching techniques. From the dc current measurement, we confirm that the nanogap formed between two microchannel junctions with approximately 80 nm depth. Using this device, we achieve the concentration volume of β-phycoerythrin protein as high as 70 pL, which is 120-fold larger than that from our previous reports, with a concentration factor as high as 10 ⁴ within 1 h. Also we show the availability of protein preconcentration under several different buffers (phosphate, acetate) at several different pH values (pH 5 to pH 9).

Original language	English
Pages (from-to)	6868-6873
Number of pages	6
Journal	Analytical chemistry
Volume	79
Issue number	17
DOIs	https://doi.org/10.1021/ac071162h
Publication status	Published - 2007 Sep 1
Externally published	Yes

ASJC Scopus subject areas

Analytical Chemistry

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title = "Poly(dimethylsiloxane)-based protein preconcentration using a nanogap generated by junction gap breakdown",

abstract = "Simple and efficient sample concentration tools are the key to the application of proteomics in a biological system. In this paper, we developed a method to realize a nanofluidic preconcentrator on a poly(dimethylsiloxane) (PDMS)-based microfluidic channel. The originality of our preconcentration device is the simple nanogap formation using the junction gap breakdown phenomenon between two PDMS microchannels, without using any photolithography and etching techniques. From the dc current measurement, we confirm that the nanogap formed between two microchannel junctions with approximately 80 nm depth. Using this device, we achieve the concentration volume of β-phycoerythrin protein as high as 70 pL, which is 120-fold larger than that from our previous reports, with a concentration factor as high as 10 4 within 1 h. Also we show the availability of protein preconcentration under several different buffers (phosphate, acetate) at several different pH values (pH 5 to pH 9).",

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T1 - Poly(dimethylsiloxane)-based protein preconcentration using a nanogap generated by junction gap breakdown

AU - Jeong, Hoon Lee

AU - Chung, Seok

AU - Sung, Jae Kim

AU - Han, Jongyoon

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AB - Simple and efficient sample concentration tools are the key to the application of proteomics in a biological system. In this paper, we developed a method to realize a nanofluidic preconcentrator on a poly(dimethylsiloxane) (PDMS)-based microfluidic channel. The originality of our preconcentration device is the simple nanogap formation using the junction gap breakdown phenomenon between two PDMS microchannels, without using any photolithography and etching techniques. From the dc current measurement, we confirm that the nanogap formed between two microchannel junctions with approximately 80 nm depth. Using this device, we achieve the concentration volume of β-phycoerythrin protein as high as 70 pL, which is 120-fold larger than that from our previous reports, with a concentration factor as high as 10 4 within 1 h. Also we show the availability of protein preconcentration under several different buffers (phosphate, acetate) at several different pH values (pH 5 to pH 9).

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Poly(dimethylsiloxane)-based protein preconcentration using a nanogap generated by junction gap breakdown

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