Poly(dimethylsiloxane)-based protein preconcentration using a nanogap generated by junction gap breakdown

Hoon Lee Jeong, Seok Chung, Jae Kim Sung, Jongyoon Han

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Simple and efficient sample concentration tools are the key to the application of proteomics in a biological system. In this paper, we developed a method to realize a nanofluidic preconcentrator on a poly(dimethylsiloxane) (PDMS)-based microfluidic channel. The originality of our preconcentration device is the simple nanogap formation using the junction gap breakdown phenomenon between two PDMS microchannels, without using any photolithography and etching techniques. From the dc current measurement, we confirm that the nanogap formed between two microchannel junctions with approximately 80 nm depth. Using this device, we achieve the concentration volume of β-phycoerythrin protein as high as 70 pL, which is 120-fold larger than that from our previous reports, with a concentration factor as high as 10 4 within 1 h. Also we show the availability of protein preconcentration under several different buffers (phosphate, acetate) at several different pH values (pH 5 to pH 9).

Original languageEnglish
Pages (from-to)6868-6873
Number of pages6
JournalAnalytical Chemistry
Volume79
Issue number17
DOIs
Publication statusPublished - 2007 Sep 1
Externally publishedYes

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Microchannels
Phycoerythrin
Nanofluidics
Photolithography
Electric current measurement
Biological systems
Microfluidics
Etching
Buffers
Proteins
Acetates
Phosphates
Availability
baysilon
Proteomics

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Poly(dimethylsiloxane)-based protein preconcentration using a nanogap generated by junction gap breakdown. / Jeong, Hoon Lee; Chung, Seok; Sung, Jae Kim; Han, Jongyoon.

In: Analytical Chemistry, Vol. 79, No. 17, 01.09.2007, p. 6868-6873.

Research output: Contribution to journalArticle

Jeong, Hoon Lee ; Chung, Seok ; Sung, Jae Kim ; Han, Jongyoon. / Poly(dimethylsiloxane)-based protein preconcentration using a nanogap generated by junction gap breakdown. In: Analytical Chemistry. 2007 ; Vol. 79, No. 17. pp. 6868-6873.
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