Abstract
Simple and efficient sample concentration tools are the key to the application of proteomics in a biological system. In this paper, we developed a method to realize a nanofluidic preconcentrator on a poly(dimethylsiloxane) (PDMS)-based microfluidic channel. The originality of our preconcentration device is the simple nanogap formation using the junction gap breakdown phenomenon between two PDMS microchannels, without using any photolithography and etching techniques. From the dc current measurement, we confirm that the nanogap formed between two microchannel junctions with approximately 80 nm depth. Using this device, we achieve the concentration volume of β-phycoerythrin protein as high as 70 pL, which is 120-fold larger than that from our previous reports, with a concentration factor as high as 10 4 within 1 h. Also we show the availability of protein preconcentration under several different buffers (phosphate, acetate) at several different pH values (pH 5 to pH 9).
| Original language | English |
|---|---|
| Pages (from-to) | 6868-6873 |
| Number of pages | 6 |
| Journal | Analytical Chemistry |
| Volume | 79 |
| Issue number | 17 |
| DOIs | |
| Publication status | Published - 2007 Sep 1 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Analytical Chemistry
Cite this
Poly(dimethylsiloxane)-based protein preconcentration using a nanogap generated by junction gap breakdown. / Jeong, Hoon Lee; Chung, Seok; Sung, Jae Kim; Han, Jongyoon.
In: Analytical Chemistry, Vol. 79, No. 17, 01.09.2007, p. 6868-6873.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Poly(dimethylsiloxane)-based protein preconcentration using a nanogap generated by junction gap breakdown
AU - Jeong, Hoon Lee
AU - Chung, Seok
AU - Sung, Jae Kim
AU - Han, Jongyoon
PY - 2007/9/1
Y1 - 2007/9/1
N2 - Simple and efficient sample concentration tools are the key to the application of proteomics in a biological system. In this paper, we developed a method to realize a nanofluidic preconcentrator on a poly(dimethylsiloxane) (PDMS)-based microfluidic channel. The originality of our preconcentration device is the simple nanogap formation using the junction gap breakdown phenomenon between two PDMS microchannels, without using any photolithography and etching techniques. From the dc current measurement, we confirm that the nanogap formed between two microchannel junctions with approximately 80 nm depth. Using this device, we achieve the concentration volume of β-phycoerythrin protein as high as 70 pL, which is 120-fold larger than that from our previous reports, with a concentration factor as high as 10 4 within 1 h. Also we show the availability of protein preconcentration under several different buffers (phosphate, acetate) at several different pH values (pH 5 to pH 9).
AB - Simple and efficient sample concentration tools are the key to the application of proteomics in a biological system. In this paper, we developed a method to realize a nanofluidic preconcentrator on a poly(dimethylsiloxane) (PDMS)-based microfluidic channel. The originality of our preconcentration device is the simple nanogap formation using the junction gap breakdown phenomenon between two PDMS microchannels, without using any photolithography and etching techniques. From the dc current measurement, we confirm that the nanogap formed between two microchannel junctions with approximately 80 nm depth. Using this device, we achieve the concentration volume of β-phycoerythrin protein as high as 70 pL, which is 120-fold larger than that from our previous reports, with a concentration factor as high as 10 4 within 1 h. Also we show the availability of protein preconcentration under several different buffers (phosphate, acetate) at several different pH values (pH 5 to pH 9).
UR - http://www.scopus.com/inward/record.url?scp=34548588442&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34548588442&partnerID=8YFLogxK
U2 - 10.1021/ac071162h
DO - 10.1021/ac071162h
M3 - Article
C2 - 17628080
AN - SCOPUS:34548588442
VL - 79
SP - 6868
EP - 6873
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 17
ER -