Polyethyleneglycol diacrylate microspheres: A novel carrier for laccase immobilisation

Xiao Yan Li, Shu Yu Yu, Hyun Jin Park, Min Zhao

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Laccase was immobilised on polyethyleneglycol diacrylate (PEGDA) microspheres. The optimal preparation conditions of PEGDA microspheres were as follows: 3.0% (w/v) 2,2-azobisisobutyro-nitrite (AIBN), 4.0-5.0% (w/v) polyvinylpyrrolidone (PVP), 5.0-8.0% (w/v) glucose and 4.0% (w/v) PEGDA in glucose solution. The volume ratio of PEGDA solution, glucose/PVP solution and AIBN solution was 25: 100: 1. Microspheres obtained exhibited good characteristics with small sizes (1-4m). The immobilised laccase showed a higher stability in a wide pH range. Thermal stability and storage stability of immobilised laccase were enhanced. The activity of immobilised laccase was 45.0% after six cycles uses. Only 62.7% of the activity remained for free laccase while there was a 60.4% increased for immobilised laccase with storage at 4°C for 25d. The Km value of laccase increased from 21.9 to 114.0mol/l after immobilisation.

Original languageEnglish
Pages (from-to)22-28
Number of pages7
JournalJournal of Microencapsulation
Volume32
Issue number1
DOIs
Publication statusPublished - 2015 Feb 1

Fingerprint

Laccase
Microspheres
immobilization
Immobilization
glucose
Glucose
nitrites
Povidone
storage stability
Nitrites
Thermodynamic stability
thermal stability
preparation
cycles
poly(ethylene glycol)diacrylate
Hot Temperature

Keywords

  • Enzyme activity
  • Immobilisation
  • Laccase
  • Polyethyleneglycol diacrylate microspheres

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Bioengineering
  • Organic Chemistry

Cite this

Polyethyleneglycol diacrylate microspheres : A novel carrier for laccase immobilisation. / Li, Xiao Yan; Yu, Shu Yu; Park, Hyun Jin; Zhao, Min.

In: Journal of Microencapsulation, Vol. 32, No. 1, 01.02.2015, p. 22-28.

Research output: Contribution to journalArticle

@article{1ed8e4a66db0424eb0a1aeefe0d6a2d9,
title = "Polyethyleneglycol diacrylate microspheres: A novel carrier for laccase immobilisation",
abstract = "Laccase was immobilised on polyethyleneglycol diacrylate (PEGDA) microspheres. The optimal preparation conditions of PEGDA microspheres were as follows: 3.0{\%} (w/v) 2,2-azobisisobutyro-nitrite (AIBN), 4.0-5.0{\%} (w/v) polyvinylpyrrolidone (PVP), 5.0-8.0{\%} (w/v) glucose and 4.0{\%} (w/v) PEGDA in glucose solution. The volume ratio of PEGDA solution, glucose/PVP solution and AIBN solution was 25: 100: 1. Microspheres obtained exhibited good characteristics with small sizes (1-4m). The immobilised laccase showed a higher stability in a wide pH range. Thermal stability and storage stability of immobilised laccase were enhanced. The activity of immobilised laccase was 45.0{\%} after six cycles uses. Only 62.7{\%} of the activity remained for free laccase while there was a 60.4{\%} increased for immobilised laccase with storage at 4°C for 25d. The Km value of laccase increased from 21.9 to 114.0mol/l after immobilisation.",
keywords = "Enzyme activity, Immobilisation, Laccase, Polyethyleneglycol diacrylate microspheres",
author = "Li, {Xiao Yan} and Yu, {Shu Yu} and Park, {Hyun Jin} and Min Zhao",
year = "2015",
month = "2",
day = "1",
doi = "10.3109/02652048.2014.940014",
language = "English",
volume = "32",
pages = "22--28",
journal = "Journal of Microencapsulation",
issn = "0265-2048",
publisher = "Informa Healthcare",
number = "1",

}

TY - JOUR

T1 - Polyethyleneglycol diacrylate microspheres

T2 - A novel carrier for laccase immobilisation

AU - Li, Xiao Yan

AU - Yu, Shu Yu

AU - Park, Hyun Jin

AU - Zhao, Min

PY - 2015/2/1

Y1 - 2015/2/1

N2 - Laccase was immobilised on polyethyleneglycol diacrylate (PEGDA) microspheres. The optimal preparation conditions of PEGDA microspheres were as follows: 3.0% (w/v) 2,2-azobisisobutyro-nitrite (AIBN), 4.0-5.0% (w/v) polyvinylpyrrolidone (PVP), 5.0-8.0% (w/v) glucose and 4.0% (w/v) PEGDA in glucose solution. The volume ratio of PEGDA solution, glucose/PVP solution and AIBN solution was 25: 100: 1. Microspheres obtained exhibited good characteristics with small sizes (1-4m). The immobilised laccase showed a higher stability in a wide pH range. Thermal stability and storage stability of immobilised laccase were enhanced. The activity of immobilised laccase was 45.0% after six cycles uses. Only 62.7% of the activity remained for free laccase while there was a 60.4% increased for immobilised laccase with storage at 4°C for 25d. The Km value of laccase increased from 21.9 to 114.0mol/l after immobilisation.

AB - Laccase was immobilised on polyethyleneglycol diacrylate (PEGDA) microspheres. The optimal preparation conditions of PEGDA microspheres were as follows: 3.0% (w/v) 2,2-azobisisobutyro-nitrite (AIBN), 4.0-5.0% (w/v) polyvinylpyrrolidone (PVP), 5.0-8.0% (w/v) glucose and 4.0% (w/v) PEGDA in glucose solution. The volume ratio of PEGDA solution, glucose/PVP solution and AIBN solution was 25: 100: 1. Microspheres obtained exhibited good characteristics with small sizes (1-4m). The immobilised laccase showed a higher stability in a wide pH range. Thermal stability and storage stability of immobilised laccase were enhanced. The activity of immobilised laccase was 45.0% after six cycles uses. Only 62.7% of the activity remained for free laccase while there was a 60.4% increased for immobilised laccase with storage at 4°C for 25d. The Km value of laccase increased from 21.9 to 114.0mol/l after immobilisation.

KW - Enzyme activity

KW - Immobilisation

KW - Laccase

KW - Polyethyleneglycol diacrylate microspheres

UR - http://www.scopus.com/inward/record.url?scp=84936764009&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84936764009&partnerID=8YFLogxK

U2 - 10.3109/02652048.2014.940014

DO - 10.3109/02652048.2014.940014

M3 - Article

C2 - 25090598

AN - SCOPUS:84936764009

VL - 32

SP - 22

EP - 28

JO - Journal of Microencapsulation

JF - Journal of Microencapsulation

SN - 0265-2048

IS - 1

ER -