Polyethyleneglycol diacrylate microspheres: A novel carrier for laccase immobilisation

Xiao Yan Li, Shu Yu Yu, Hyun Jin Park, Min Zhao

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Laccase was immobilised on polyethyleneglycol diacrylate (PEGDA) microspheres. The optimal preparation conditions of PEGDA microspheres were as follows: 3.0% (w/v) 2,2-azobisisobutyro-nitrite (AIBN), 4.0-5.0% (w/v) polyvinylpyrrolidone (PVP), 5.0-8.0% (w/v) glucose and 4.0% (w/v) PEGDA in glucose solution. The volume ratio of PEGDA solution, glucose/PVP solution and AIBN solution was 25: 100: 1. Microspheres obtained exhibited good characteristics with small sizes (1-4m). The immobilised laccase showed a higher stability in a wide pH range. Thermal stability and storage stability of immobilised laccase were enhanced. The activity of immobilised laccase was 45.0% after six cycles uses. Only 62.7% of the activity remained for free laccase while there was a 60.4% increased for immobilised laccase with storage at 4°C for 25d. The Km value of laccase increased from 21.9 to 114.0mol/l after immobilisation.

Original languageEnglish
Pages (from-to)22-28
Number of pages7
JournalJournal of Microencapsulation
Volume32
Issue number1
DOIs
Publication statusPublished - 2015 Feb 1

Keywords

  • Enzyme activity
  • Immobilisation
  • Laccase
  • Polyethyleneglycol diacrylate microspheres

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Bioengineering
  • Organic Chemistry

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