Polymorphism and expression of isoflavone synthase genes from soybean cultivars

Hyo Kyoung Kim, Yun Hee Jang, Il Sun Baek, Jeong Hwan Lee, Min Joo Park, Young Soo Chung, Jong Il Chung, Jeong Kook Kim

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Isoflavones are synthesized by isoflavone synthases via the phenylpropanoid pathway in legumes. We have cloned two isoflavone synthase genes, IFS1 and IFS2, from a total of 18 soybean cultivars. The amino acid residues of the proteins that differed between cultivars were dispersed over the entire coding region. However, amino acid sequence variation did not occur in conserved domains such as the ERR triad region, except that one conserved amino acid was changed in the IFS2 protein of the GS12 cultivar (R 374G) and the IFS1 proteins of the 99M06 and Soja99s65 cultivars (A 109T, F 105I). In three cultivars (99M06, 99M116, and Simheukpi), most of amino acid changes were such that the difference between the amino acid sequences of IFS1 and IFS2 was reduced. The expression profiles of three enzymes that convert naringenin to the isoflavone, genistein, chalcone isomerase (CHI), isoflavone synthase (IFS) and flavanone 3-hydroxylase (F3H) were examined. In general, IFS mRNA was more abundant in etiolated seedlings than mature plants whereas the levels of CHI and F3H mRNAs were similar in the two stages. During seed development, IFS was expressed a little later than CHI and F3H but expression of these three genes was barely detectable, if at all, during later seed hardening. In addition, we found that the levels of CHI, F3H, and IFS mRNAs were under circadian control. We also showed that IFS was induced by wounding and by application of methyl jasmonate to etiolated soybean seedlings.

Original languageEnglish
Pages (from-to)67-73
Number of pages7
JournalMolecules and Cells
Volume19
Issue number1
Publication statusPublished - 2005 Dec 1

Fingerprint

chalcone isomerase
Soybeans
Genes
Isoflavones
Seedlings
Amino Acids
Messenger RNA
Amino Acid Sequence
Seeds
Proteins
Genistein
isoflavone synthase
Fabaceae
Gene Expression
flavanone 3-dioxygenase
Enzymes

Keywords

  • Abiotic Stress
  • Circadian Regulation
  • Isoflavone Synthase
  • Phenylpropanoid
  • Polymorphism
  • Soybean Cultivars

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Kim, H. K., Jang, Y. H., Baek, I. S., Lee, J. H., Park, M. J., Chung, Y. S., ... Kim, J. K. (2005). Polymorphism and expression of isoflavone synthase genes from soybean cultivars. Molecules and Cells, 19(1), 67-73.

Polymorphism and expression of isoflavone synthase genes from soybean cultivars. / Kim, Hyo Kyoung; Jang, Yun Hee; Baek, Il Sun; Lee, Jeong Hwan; Park, Min Joo; Chung, Young Soo; Chung, Jong Il; Kim, Jeong Kook.

In: Molecules and Cells, Vol. 19, No. 1, 01.12.2005, p. 67-73.

Research output: Contribution to journalArticle

Kim, HK, Jang, YH, Baek, IS, Lee, JH, Park, MJ, Chung, YS, Chung, JI & Kim, JK 2005, 'Polymorphism and expression of isoflavone synthase genes from soybean cultivars', Molecules and Cells, vol. 19, no. 1, pp. 67-73.
Kim HK, Jang YH, Baek IS, Lee JH, Park MJ, Chung YS et al. Polymorphism and expression of isoflavone synthase genes from soybean cultivars. Molecules and Cells. 2005 Dec 1;19(1):67-73.
Kim, Hyo Kyoung ; Jang, Yun Hee ; Baek, Il Sun ; Lee, Jeong Hwan ; Park, Min Joo ; Chung, Young Soo ; Chung, Jong Il ; Kim, Jeong Kook. / Polymorphism and expression of isoflavone synthase genes from soybean cultivars. In: Molecules and Cells. 2005 ; Vol. 19, No. 1. pp. 67-73.
@article{754e9b753a284c949e1e151b7eceb34c,
title = "Polymorphism and expression of isoflavone synthase genes from soybean cultivars",
abstract = "Isoflavones are synthesized by isoflavone synthases via the phenylpropanoid pathway in legumes. We have cloned two isoflavone synthase genes, IFS1 and IFS2, from a total of 18 soybean cultivars. The amino acid residues of the proteins that differed between cultivars were dispersed over the entire coding region. However, amino acid sequence variation did not occur in conserved domains such as the ERR triad region, except that one conserved amino acid was changed in the IFS2 protein of the GS12 cultivar (R 374G) and the IFS1 proteins of the 99M06 and Soja99s65 cultivars (A 109T, F 105I). In three cultivars (99M06, 99M116, and Simheukpi), most of amino acid changes were such that the difference between the amino acid sequences of IFS1 and IFS2 was reduced. The expression profiles of three enzymes that convert naringenin to the isoflavone, genistein, chalcone isomerase (CHI), isoflavone synthase (IFS) and flavanone 3-hydroxylase (F3H) were examined. In general, IFS mRNA was more abundant in etiolated seedlings than mature plants whereas the levels of CHI and F3H mRNAs were similar in the two stages. During seed development, IFS was expressed a little later than CHI and F3H but expression of these three genes was barely detectable, if at all, during later seed hardening. In addition, we found that the levels of CHI, F3H, and IFS mRNAs were under circadian control. We also showed that IFS was induced by wounding and by application of methyl jasmonate to etiolated soybean seedlings.",
keywords = "Abiotic Stress, Circadian Regulation, Isoflavone Synthase, Phenylpropanoid, Polymorphism, Soybean Cultivars",
author = "Kim, {Hyo Kyoung} and Jang, {Yun Hee} and Baek, {Il Sun} and Lee, {Jeong Hwan} and Park, {Min Joo} and Chung, {Young Soo} and Chung, {Jong Il} and Kim, {Jeong Kook}",
year = "2005",
month = "12",
day = "1",
language = "English",
volume = "19",
pages = "67--73",
journal = "Molecules and Cells",
issn = "1016-8478",
publisher = "Korean Society for Molecular and Cellular Biology",
number = "1",

}

TY - JOUR

T1 - Polymorphism and expression of isoflavone synthase genes from soybean cultivars

AU - Kim, Hyo Kyoung

AU - Jang, Yun Hee

AU - Baek, Il Sun

AU - Lee, Jeong Hwan

AU - Park, Min Joo

AU - Chung, Young Soo

AU - Chung, Jong Il

AU - Kim, Jeong Kook

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Isoflavones are synthesized by isoflavone synthases via the phenylpropanoid pathway in legumes. We have cloned two isoflavone synthase genes, IFS1 and IFS2, from a total of 18 soybean cultivars. The amino acid residues of the proteins that differed between cultivars were dispersed over the entire coding region. However, amino acid sequence variation did not occur in conserved domains such as the ERR triad region, except that one conserved amino acid was changed in the IFS2 protein of the GS12 cultivar (R 374G) and the IFS1 proteins of the 99M06 and Soja99s65 cultivars (A 109T, F 105I). In three cultivars (99M06, 99M116, and Simheukpi), most of amino acid changes were such that the difference between the amino acid sequences of IFS1 and IFS2 was reduced. The expression profiles of three enzymes that convert naringenin to the isoflavone, genistein, chalcone isomerase (CHI), isoflavone synthase (IFS) and flavanone 3-hydroxylase (F3H) were examined. In general, IFS mRNA was more abundant in etiolated seedlings than mature plants whereas the levels of CHI and F3H mRNAs were similar in the two stages. During seed development, IFS was expressed a little later than CHI and F3H but expression of these three genes was barely detectable, if at all, during later seed hardening. In addition, we found that the levels of CHI, F3H, and IFS mRNAs were under circadian control. We also showed that IFS was induced by wounding and by application of methyl jasmonate to etiolated soybean seedlings.

AB - Isoflavones are synthesized by isoflavone synthases via the phenylpropanoid pathway in legumes. We have cloned two isoflavone synthase genes, IFS1 and IFS2, from a total of 18 soybean cultivars. The amino acid residues of the proteins that differed between cultivars were dispersed over the entire coding region. However, amino acid sequence variation did not occur in conserved domains such as the ERR triad region, except that one conserved amino acid was changed in the IFS2 protein of the GS12 cultivar (R 374G) and the IFS1 proteins of the 99M06 and Soja99s65 cultivars (A 109T, F 105I). In three cultivars (99M06, 99M116, and Simheukpi), most of amino acid changes were such that the difference between the amino acid sequences of IFS1 and IFS2 was reduced. The expression profiles of three enzymes that convert naringenin to the isoflavone, genistein, chalcone isomerase (CHI), isoflavone synthase (IFS) and flavanone 3-hydroxylase (F3H) were examined. In general, IFS mRNA was more abundant in etiolated seedlings than mature plants whereas the levels of CHI and F3H mRNAs were similar in the two stages. During seed development, IFS was expressed a little later than CHI and F3H but expression of these three genes was barely detectable, if at all, during later seed hardening. In addition, we found that the levels of CHI, F3H, and IFS mRNAs were under circadian control. We also showed that IFS was induced by wounding and by application of methyl jasmonate to etiolated soybean seedlings.

KW - Abiotic Stress

KW - Circadian Regulation

KW - Isoflavone Synthase

KW - Phenylpropanoid

KW - Polymorphism

KW - Soybean Cultivars

UR - http://www.scopus.com/inward/record.url?scp=22844444073&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=22844444073&partnerID=8YFLogxK

M3 - Article

VL - 19

SP - 67

EP - 73

JO - Molecules and Cells

JF - Molecules and Cells

SN - 1016-8478

IS - 1

ER -