Polypyrimidine tract-binding protein interacts with HnRNP L

Bumsuk Hahm, Ook H. Cho, Jung E. Kim, Yoon K. Kim, Jong H. Kim, Young L. Oh, Sung K. Jang

Research output: Contribution to journalArticlepeer-review

67 Citations (Scopus)

Abstract

Polypyrimidine tract-binding protein (PTB) is involved in pre-mRNA splicing and internal ribosomal entry site (IRES)-dependent translation. In order to identify cellular protein(s) interacting with PTB, we performed a yeast two-hybrid screening. Heterogeneous nuclear ribonucleoprotein L (hnRNP L) was identified as a PTB-binding protein. The interaction between PTB and hnRNP L was confirmed in an in vitro binding assay. Both PTB and hnRNP L were found to localize in the nucleoplasm, excepting the nucleoli, in HeLa cells by the green fluorescent protein (GFP)-fused protein detection method. The N-terminal half of PTB (aa 1-329) and most of hnRNP L (aa 141-558) is required for the interaction between PTB and hnRNP L.

Original languageEnglish
Pages (from-to)401-406
Number of pages6
JournalFEBS Letters
Volume425
Issue number3
DOIs
Publication statusPublished - 1998 Apr 3
Externally publishedYes

Keywords

  • Heterogenous nuclear ribonucleoprotein L
  • Polypyrimidine tract-binding protein
  • Protein-protein interaction

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Polypyrimidine tract-binding protein interacts with HnRNP L'. Together they form a unique fingerprint.

Cite this