Polypyrimidine tract-binding protein interacts with HnRNP L

Bumsuk Hahm, Ook H. Cho, Jung E. Kim, Yoon Ki Kim, Jong H. Kim, Young L. Oh, Sung K. Jang

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Polypyrimidine tract-binding protein (PTB) is involved in pre-mRNA splicing and internal ribosomal entry site (IRES)-dependent translation. In order to identify cellular protein(s) interacting with PTB, we performed a yeast two-hybrid screening. Heterogeneous nuclear ribonucleoprotein L (hnRNP L) was identified as a PTB-binding protein. The interaction between PTB and hnRNP L was confirmed in an in vitro binding assay. Both PTB and hnRNP L were found to localize in the nucleoplasm, excepting the nucleoli, in HeLa cells by the green fluorescent protein (GFP)-fused protein detection method. The N-terminal half of PTB (aa 1-329) and most of hnRNP L (aa 141-558) is required for the interaction between PTB and hnRNP L.

Original languageEnglish
Pages (from-to)401-406
Number of pages6
JournalFEBS Letters
Volume425
Issue number3
DOIs
Publication statusPublished - 1998 Apr 3
Externally publishedYes

Fingerprint

Polypyrimidine Tract-Binding Protein
Heterogeneous-Nuclear Ribonucleoprotein L
RNA Precursors
Green Fluorescent Proteins
HeLa Cells
Protein Binding
Yeast
Assays
Carrier Proteins
Screening
Proteins
Yeasts

Keywords

  • Heterogenous nuclear ribonucleoprotein L
  • Polypyrimidine tract-binding protein
  • Protein-protein interaction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Hahm, B., Cho, O. H., Kim, J. E., Kim, Y. K., Kim, J. H., Oh, Y. L., & Jang, S. K. (1998). Polypyrimidine tract-binding protein interacts with HnRNP L. FEBS Letters, 425(3), 401-406. https://doi.org/10.1016/S0014-5793(98)00269-5

Polypyrimidine tract-binding protein interacts with HnRNP L. / Hahm, Bumsuk; Cho, Ook H.; Kim, Jung E.; Kim, Yoon Ki; Kim, Jong H.; Oh, Young L.; Jang, Sung K.

In: FEBS Letters, Vol. 425, No. 3, 03.04.1998, p. 401-406.

Research output: Contribution to journalArticle

Hahm, B, Cho, OH, Kim, JE, Kim, YK, Kim, JH, Oh, YL & Jang, SK 1998, 'Polypyrimidine tract-binding protein interacts with HnRNP L', FEBS Letters, vol. 425, no. 3, pp. 401-406. https://doi.org/10.1016/S0014-5793(98)00269-5
Hahm, Bumsuk ; Cho, Ook H. ; Kim, Jung E. ; Kim, Yoon Ki ; Kim, Jong H. ; Oh, Young L. ; Jang, Sung K. / Polypyrimidine tract-binding protein interacts with HnRNP L. In: FEBS Letters. 1998 ; Vol. 425, No. 3. pp. 401-406.
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